Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture

Ying Ju Lin, Chia Yen Chen, Kuan Teh Jeang, Xiang Liu, Jen Hsien Wang, Chien Hui Hung, Hsinyi Tsang, Ting Hsu Lin, Chiu Chu Liao, Shao Mei Huang, Cheng Wen Lin, Mao Wang Ho, Wen Kuei Chien, Jin Hua Chen, Tsung Jung Ho, Fuu Jen Tsai

Research output: Contribution to journalArticle

Abstract

Background: The human immunodeficiency virus (HIV-1) exploits host proteins to complete its life cycle. Genome-wide siRNA approaches suggested that host proteins affect HIV-1 replication. However, the results barely overlapped. RING finger protein 39 (RNF39) has been identified from genome-wide association studies. However, its function during HIV-1 replication remains unclear. Methods and results: We investigated the relationship between common RNF39 genetic variants and HIV-1 viral loads. The effect of RNF39 protein knockdown or overexpression on HIV-1 replication was then investigated in different cell lines. Two genetic variants were associated with HIV-1 viral loads. Patients with the ht1-GG/GG haplotype presented lower RNF39 expression levels and lower HIV-1 viral load. RNF39 knockdown inhibited HIV-1 expression. Conclusions: RNF39 protein may be involved in HIV-1 replication as observed in genetic studies on patients with HIV-1 and in in vitro cell cultures.

Original languageEnglish
Article number40
JournalCell and Bioscience
Volume4
Issue number1
DOIs
Publication statusPublished - Aug 5 2014

Fingerprint

Viral Load
Cell culture
Fingers
HIV-1
Cell Culture Techniques
Plasmas
Proteins
Genes
Human Immunodeficiency Virus Proteins
Viruses
Genome-Wide Association Study
Small Interfering RNA
Life cycle
Life Cycle Stages
Haplotypes
Cells
Genome
Cell Line

Keywords

  • HIV-1 viral load
  • RNF39
  • Single nucleotide polymorphism
  • Viral replication

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture. / Lin, Ying Ju; Chen, Chia Yen; Jeang, Kuan Teh; Liu, Xiang; Wang, Jen Hsien; Hung, Chien Hui; Tsang, Hsinyi; Lin, Ting Hsu; Liao, Chiu Chu; Huang, Shao Mei; Lin, Cheng Wen; Ho, Mao Wang; Chien, Wen Kuei; Chen, Jin Hua; Ho, Tsung Jung; Tsai, Fuu Jen.

In: Cell and Bioscience, Vol. 4, No. 1, 40, 05.08.2014.

Research output: Contribution to journalArticle

Lin, YJ, Chen, CY, Jeang, KT, Liu, X, Wang, JH, Hung, CH, Tsang, H, Lin, TH, Liao, CC, Huang, SM, Lin, CW, Ho, MW, Chien, WK, Chen, JH, Ho, TJ & Tsai, FJ 2014, 'Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture', Cell and Bioscience, vol. 4, no. 1, 40. https://doi.org/10.1186/2045-3701-4-40
Lin, Ying Ju ; Chen, Chia Yen ; Jeang, Kuan Teh ; Liu, Xiang ; Wang, Jen Hsien ; Hung, Chien Hui ; Tsang, Hsinyi ; Lin, Ting Hsu ; Liao, Chiu Chu ; Huang, Shao Mei ; Lin, Cheng Wen ; Ho, Mao Wang ; Chien, Wen Kuei ; Chen, Jin Hua ; Ho, Tsung Jung ; Tsai, Fuu Jen. / Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture. In: Cell and Bioscience. 2014 ; Vol. 4, No. 1.
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abstract = "Background: The human immunodeficiency virus (HIV-1) exploits host proteins to complete its life cycle. Genome-wide siRNA approaches suggested that host proteins affect HIV-1 replication. However, the results barely overlapped. RING finger protein 39 (RNF39) has been identified from genome-wide association studies. However, its function during HIV-1 replication remains unclear. Methods and results: We investigated the relationship between common RNF39 genetic variants and HIV-1 viral loads. The effect of RNF39 protein knockdown or overexpression on HIV-1 replication was then investigated in different cell lines. Two genetic variants were associated with HIV-1 viral loads. Patients with the ht1-GG/GG haplotype presented lower RNF39 expression levels and lower HIV-1 viral load. RNF39 knockdown inhibited HIV-1 expression. Conclusions: RNF39 protein may be involved in HIV-1 replication as observed in genetic studies on patients with HIV-1 and in in vitro cell cultures.",
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T1 - Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture

AU - Lin, Ying Ju

AU - Chen, Chia Yen

AU - Jeang, Kuan Teh

AU - Liu, Xiang

AU - Wang, Jen Hsien

AU - Hung, Chien Hui

AU - Tsang, Hsinyi

AU - Lin, Ting Hsu

AU - Liao, Chiu Chu

AU - Huang, Shao Mei

AU - Lin, Cheng Wen

AU - Ho, Mao Wang

AU - Chien, Wen Kuei

AU - Chen, Jin Hua

AU - Ho, Tsung Jung

AU - Tsai, Fuu Jen

PY - 2014/8/5

Y1 - 2014/8/5

N2 - Background: The human immunodeficiency virus (HIV-1) exploits host proteins to complete its life cycle. Genome-wide siRNA approaches suggested that host proteins affect HIV-1 replication. However, the results barely overlapped. RING finger protein 39 (RNF39) has been identified from genome-wide association studies. However, its function during HIV-1 replication remains unclear. Methods and results: We investigated the relationship between common RNF39 genetic variants and HIV-1 viral loads. The effect of RNF39 protein knockdown or overexpression on HIV-1 replication was then investigated in different cell lines. Two genetic variants were associated with HIV-1 viral loads. Patients with the ht1-GG/GG haplotype presented lower RNF39 expression levels and lower HIV-1 viral load. RNF39 knockdown inhibited HIV-1 expression. Conclusions: RNF39 protein may be involved in HIV-1 replication as observed in genetic studies on patients with HIV-1 and in in vitro cell cultures.

AB - Background: The human immunodeficiency virus (HIV-1) exploits host proteins to complete its life cycle. Genome-wide siRNA approaches suggested that host proteins affect HIV-1 replication. However, the results barely overlapped. RING finger protein 39 (RNF39) has been identified from genome-wide association studies. However, its function during HIV-1 replication remains unclear. Methods and results: We investigated the relationship between common RNF39 genetic variants and HIV-1 viral loads. The effect of RNF39 protein knockdown or overexpression on HIV-1 replication was then investigated in different cell lines. Two genetic variants were associated with HIV-1 viral loads. Patients with the ht1-GG/GG haplotype presented lower RNF39 expression levels and lower HIV-1 viral load. RNF39 knockdown inhibited HIV-1 expression. Conclusions: RNF39 protein may be involved in HIV-1 replication as observed in genetic studies on patients with HIV-1 and in in vitro cell cultures.

KW - HIV-1 viral load

KW - RNF39

KW - Single nucleotide polymorphism

KW - Viral replication

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