Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture

Ying Ju Lin, Chia Yen Chen, Kuan Teh Jeang, Xiang Liu, Jen Hsien Wang, Chien Hui Hung, Hsinyi Tsang, Ting Hsu Lin, Chiu Chu Liao, Shao Mei Huang, Cheng Wen Lin, Mao Wang Ho, Wen Kuei Chien, Jin Hua Chen, Tsung Jung Ho, Fuu Jen Tsai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: The human immunodeficiency virus (HIV-1) exploits host proteins to complete its life cycle. Genome-wide siRNA approaches suggested that host proteins affect HIV-1 replication. However, the results barely overlapped. RING finger protein 39 (RNF39) has been identified from genome-wide association studies. However, its function during HIV-1 replication remains unclear. Methods and results: We investigated the relationship between common RNF39 genetic variants and HIV-1 viral loads. The effect of RNF39 protein knockdown or overexpression on HIV-1 replication was then investigated in different cell lines. Two genetic variants were associated with HIV-1 viral loads. Patients with the ht1-GG/GG haplotype presented lower RNF39 expression levels and lower HIV-1 viral load. RNF39 knockdown inhibited HIV-1 expression. Conclusions: RNF39 protein may be involved in HIV-1 replication as observed in genetic studies on patients with HIV-1 and in in vitro cell cultures.

Original languageEnglish
Article number40
JournalCell and Bioscience
Volume4
Issue number1
DOIs
Publication statusPublished - Aug 5 2014

Keywords

  • HIV-1 viral load
  • RNF39
  • Single nucleotide polymorphism
  • Viral replication

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Lin, Y. J., Chen, C. Y., Jeang, K. T., Liu, X., Wang, J. H., Hung, C. H., Tsang, H., Lin, T. H., Liao, C. C., Huang, S. M., Lin, C. W., Ho, M. W., Chien, W. K., Chen, J. H., Ho, T. J., & Tsai, F. J. (2014). Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture. Cell and Bioscience, 4(1), [40]. https://doi.org/10.1186/2045-3701-4-40