Riboflavin protects mice against liposaccharide-induced shock through expression of heat shock protein 25

Chun Kuang Shih, Chiao-Ming Chen, C. Y Oliver Chen, Jen-Fang Liu, Hui Wen Lin, Hung Tsung Chou, Sing Chung Li

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Riboflavin (vitamin B2) is a water-soluble vitamin essential for normal cellular functions, growth and development. This study aimed to investigate the effects of vitamin B2 on the survival rate, and expressions of tissue heat shock protein 25 (HSP25) and heat shock factor 1 (HSF1) in mice undergoing lipopolysaccharide (LPS) induced shock. Mice were assigned to four groups, saline vehicle, LPS, LPS plus low dose of vitamin B2 (LB2) and LPS plus high dose of vitamin B2 (HB2). Vitamin B2 (1 and 10. mg/kg BW) was administered intraperitoneally at 2 and 0. h before the i.p. administration of LPS. At the end of the experiment, the survival rate monitored was 10, 20, 60, and 100% for LPS, LB2, HB2, and saline mice, respectively. HSP25 expressions in the heart and lung were significantly enhanced in a time-dependent manner in the HB2 mice as compared to the saline mice (p<0.05), but not altered in the LB2 mice. In the HB2 mice, plasma riboflavin concentrations reached 300. nM at 6. h post LPS and returned to the 0. h level at 72. h. The results showed that high dose of riboflavin could decrease LPS-induced mortality through an increased expression of HSP25.

Original languageEnglish
Pages (from-to)1913-1918
Number of pages6
JournalFood and Chemical Toxicology
Volume48
Issue number7
DOIs
Publication statusPublished - Jul 2010

Keywords

  • Heat shock factor 1
  • Heat shock protein 25
  • Lipopolysaccharide
  • Sepsis
  • Vitamin B2

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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