Riboflavin at High Doses Enhances Lung Cancer Cell Proliferation, Invasion, and Migration

Hui Ting Yang, Pei Chun Chao, Mei Chin Yin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The influence of riboflavin (vitamin B2) upon growth, invasion, and migration in non-small cell lung cancer cell lines was evaluated. Riboflavin at 1, 10, 25, 50, 100, 200, or 400 μmol/L was added into A549, H3255, or Calu-6 cells. The effects of this compound upon level and/or expression of reactive oxygen species (ROS), inflammatory cytokines, intercellular adhesion molecule (ICAM)-1, fibronectin, matrix metalloproteinase (MMP)-9, MMP-2, focal adhesion kinase (FAK), nuclear factor kappa B (NF-κB), and mitogen-activated protein kinase (MAPK) were examined. Results showed that riboflavin at test doses did not affect the level of ROS and glutathione. Riboflavin at 200 and 400 μmol/L significantly enhanced cell growth in test lung cancer cell lines, and at 400 μmol/L significantly increased the release of interleukin-6, tumor necrosis factor-alpha, and vascular endothelial growth factor. This agent at 200 and 400 μmol/L also upregulated protein production of ICAM-1, fibronectin, MMP-9, MMP-2, NF-κB p50, p-p38 MAPK, and FAK; and at 400 μmol/L enhanced invasion and migration in test cell lines. These findings suggested that riboflavin at high doses might promote lung cancer progression.

Original languageEnglish
JournalJournal of Food Science
Volume78
Issue number2
DOIs
Publication statusPublished - Feb 1 2013
Externally publishedYes

Fingerprint

Riboflavin
lung neoplasms
riboflavin
Cell Movement
Lung Neoplasms
cell proliferation
Cell Proliferation
non-specific protein-tyrosine kinase
dosage
Focal Adhesion Protein-Tyrosine Kinases
gelatinase A
gelatinase B
NF-kappa B
fibronectins
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
cell lines
Intercellular Adhesion Molecule-1
mitogen-activated protein kinase
Fibronectins

Keywords

  • Cytokines
  • Focal adhesion kinase
  • Migration
  • Non-small cell lung cancer
  • Riboflavin

ASJC Scopus subject areas

  • Food Science

Cite this

Riboflavin at High Doses Enhances Lung Cancer Cell Proliferation, Invasion, and Migration. / Yang, Hui Ting; Chao, Pei Chun; Yin, Mei Chin.

In: Journal of Food Science, Vol. 78, No. 2, 01.02.2013.

Research output: Contribution to journalArticle

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N2 - The influence of riboflavin (vitamin B2) upon growth, invasion, and migration in non-small cell lung cancer cell lines was evaluated. Riboflavin at 1, 10, 25, 50, 100, 200, or 400 μmol/L was added into A549, H3255, or Calu-6 cells. The effects of this compound upon level and/or expression of reactive oxygen species (ROS), inflammatory cytokines, intercellular adhesion molecule (ICAM)-1, fibronectin, matrix metalloproteinase (MMP)-9, MMP-2, focal adhesion kinase (FAK), nuclear factor kappa B (NF-κB), and mitogen-activated protein kinase (MAPK) were examined. Results showed that riboflavin at test doses did not affect the level of ROS and glutathione. Riboflavin at 200 and 400 μmol/L significantly enhanced cell growth in test lung cancer cell lines, and at 400 μmol/L significantly increased the release of interleukin-6, tumor necrosis factor-alpha, and vascular endothelial growth factor. This agent at 200 and 400 μmol/L also upregulated protein production of ICAM-1, fibronectin, MMP-9, MMP-2, NF-κB p50, p-p38 MAPK, and FAK; and at 400 μmol/L enhanced invasion and migration in test cell lines. These findings suggested that riboflavin at high doses might promote lung cancer progression.

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