Rhododendron oldhamii leaf extract improves fatty liver syndrome by increasing lipid oxidation and decreasing the lipogenesis pathway in mice

Ya Ling Liu, Lei Chen Lin, Yu Tang Tung, Shang Tse Ho, Yao Li Chen, Chi Chen Lin, Jyh Horng Wu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Some members of Rhododendron genus are traditionally used as medicinal plants for arthritis, acute and chronic bronchitis, asthma, pain, inflammation, rheumatism, hypertension and metabolic diseases. To the best of our knowledge, there is no report on the protective effects of R. oldhamii leaf extract on non-alcoholic fatty liver disease (NAFLD) in vivo and in vitro. In this study, the effects of R. oldhamii leaf extract on inhibiting the free fatty acid (FFA)-induced accumulation of fat in HepG2 cells and on improving fatty liver syndrome in mice with high fat diet (HFD)-induced NAFLD were investigated. For the in vitro assay, HepG2 cells were treated with FFAs (oleate/palmitate = 2:1) with or without treatment with R. oldhamii leaf ethyl acetate (EtOAc) fraction to observe lipid accumulation using Nile red and oil red O stains. For the in vivo assay, C57BL/6 mice were randomly assigned to three groups (n = 5), including the normal diet group, the HFD group and the HFD+EtOAc group. After 11 weeks, body weight, serum biochemical indices and the mRNA expressions of the liver tissue, as well as the outward appearance, weight and histopathological analysis of liver and adipose tissues were evaluated. Among the fractions derived from R. oldhamii leaf, the EtOAc fraction exhibited a strong fat-accumulation inhibitory activity. Following reverse-phase high-performance liquid chromatography (HPLC), four specific phytochemicals, including (2R, 3R)-astilbin (AS), hyposide (HY), guaijaverin (GU) and quercitrin (QU), were isolated and identified from the EtOAc fraction of R. oldhamii leaf extract. Among them, AS and HY showed excellent fat-accumulation inhibitory activity. Thus, the EtOAc fraction of R. oldhamii leaf and its derived phytochemicals have great potential in preventing FFA-induced fat accumulation. In addition, the EtOAc fraction of R. oldhamii leaf significantly improved fatty liver syndrome and reduced total cholesterol (TC) and triglyceride (TG) in HFD-induced NAFLD mice at a dosage of 200 mg/kg BW. These results demonstrated that the methanolic extracts from R. oldhamii leaf have excellent inhibitory activities against fat accumulation and anti-NAFLD activities and thus have great potential as a natural health product.

Original languageEnglish
Pages (from-to)862-870
Number of pages9
JournalInternational Journal of Medical Sciences
Volume14
Issue number9
DOIs
Publication statusPublished - 2017

Fingerprint

Rhododendron
Lipogenesis
Fatty Liver
High Fat Diet
Fats
Lipids
Hep G2 Cells
Phytochemicals
Nonesterified Fatty Acids
Palmitates
Liver
Chronic Bronchitis
Metabolic Diseases
Reverse-Phase Chromatography
Oleic Acid
Medicinal Plants
Rheumatic Diseases
Inbred C57BL Mouse
Arthritis
Adipose Tissue

Keywords

  • Fat accumulation
  • Free fatty acid (FFA)
  • High fat diet (HFD)
  • Non-alcoholic fatty liver disease (NAFLD
  • Rhododendron oldhamii

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Rhododendron oldhamii leaf extract improves fatty liver syndrome by increasing lipid oxidation and decreasing the lipogenesis pathway in mice. / Liu, Ya Ling; Lin, Lei Chen; Tung, Yu Tang; Ho, Shang Tse; Chen, Yao Li; Lin, Chi Chen; Wu, Jyh Horng.

In: International Journal of Medical Sciences, Vol. 14, No. 9, 2017, p. 862-870.

Research output: Contribution to journalArticle

Liu, Ya Ling ; Lin, Lei Chen ; Tung, Yu Tang ; Ho, Shang Tse ; Chen, Yao Li ; Lin, Chi Chen ; Wu, Jyh Horng. / Rhododendron oldhamii leaf extract improves fatty liver syndrome by increasing lipid oxidation and decreasing the lipogenesis pathway in mice. In: International Journal of Medical Sciences. 2017 ; Vol. 14, No. 9. pp. 862-870.
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