Revisiting with a relative-density calibration approach the determination of growth rates of microorganisms by use of optical density data from liquid cultures

Hsiu Li Lin, Chien Chung Lin, Yi Jen Lin, Hsiu-Chen Lin, Chwen-Ming Shih, Chi Rong Chen, Rong Nan Huang, Tai-Chih Kuo

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Abstract

To solve the problems of measuring the growth rates of microorganisms from optical density (OD)-growth time plots, we used relative-density (RD) plots. The relationship of OD and RD was built from the diluted grown cultures. This method was satisfactorily applied to study the growth of Escherichia coli and the cyanobacterium Anabaena spiroides.

Original languageEnglish
Pages (from-to)1683-1685
Number of pages3
JournalApplied and Environmental Microbiology
Volume76
Issue number5
DOIs
Publication statusPublished - Mar 2010

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ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

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