Revisiting with a relative-density calibration approach the determination of growth rates of microorganisms by use of optical density data from liquid cultures

Hsiu Li Lin, Chien Chung Lin, Yi Jen Lin, Hsiu-Chen Lin, Chwen-Ming Shih, Chi Rong Chen, Rong Nan Huang, Tai-Chih Kuo

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14 Citations (Scopus)


To solve the problems of measuring the growth rates of microorganisms from optical density (OD)-growth time plots, we used relative-density (RD) plots. The relationship of OD and RD was built from the diluted grown cultures. This method was satisfactorily applied to study the growth of Escherichia coli and the cyanobacterium Anabaena spiroides.

Original languageEnglish
Pages (from-to)1683-1685
Number of pages3
JournalApplied and Environmental Microbiology
Issue number5
Publication statusPublished - Mar 2010


ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

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