Reversed CD4/CD8 ratios of tumor-infiltrating lymphocytes are correlated with the progression of human cervical carcinoma

Bor Ching Sheu, Su Ming Hsu, Hong Nerng Ho, Rong Hwa Lin, Pao Ling Torng, Su Cheng Huang

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

BACKGROUND. To investigate the clinical significance of tumor- infiltrating lymphocytes (TILs)within the tumor milieu of human cervical carcinoma, the authors quantitatively measured and compared the subpopulations of lymphocytes infiltrating the neoplastic cervix. METHODS. A total of 30 patients with Stage Ia-IIa cervical carcinoma were enrolled. TILs were isolated from tissue specimens by means of a mechanical dispersal technique, and the immunocyte subsets were quantified with dual-color flow cytometry. Bulky tumor was defined as tumor size >4 cm in greatest dimension according to the 1995 staging of the International Federation of Gynecology and Obstetrics. RESULTS. The CD4/CD8 ratios of TILs were reversed in both cervical squamous cell carcinoma in = 20) and cervical adenocarcinoma in = 10). The proportion of CD4+ T cells was significantly lower in tumors from patients with lymph node metastasis (n = 8) than in those from patients without lymph node metastasis in = 22) (24.5 vs. 32.7, P = 0.001), as was the reversed CD4/CD8 ratio (0.50 vs. 0.81, P = 0.001). The proportion of CD4+ T cells was much lower in bulky tumors (n = 5) than in nonbulky tumors in = 25) (21.4 vs. 32.5, P < 0 001), reflecting in a more strongly reversed CD4/CD8 ratio (0.41 vs. 0.81, P = 0.001). CONCLUSIONS. Decreased proportions of tumor-infiltrating CD4+ T cells with reversed CD4/CD8 ratios are highly correlated with rapid tumor growth and lymph node metastasis in cervical carcinoma. The regional immune escape is of prognostic importance with regard to cancer progression.

Original languageEnglish
Pages (from-to)1537-1543
Number of pages7
JournalCancer
Volume86
Issue number8
DOIs
Publication statusPublished - Oct 15 1999
Externally publishedYes

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Tumor-Infiltrating Lymphocytes
CD4-CD8 Ratio
Carcinoma
Neoplasms
Lymph Nodes
Neoplasm Metastasis
T-Lymphocytes
Lymphocyte Subsets
Gynecology
Cervix Uteri
Obstetrics
Squamous Cell Carcinoma
Flow Cytometry
Adenocarcinoma
Color

Keywords

  • CD4/CD8 ratio
  • Cervical carcinoma
  • Lymph node metastasis
  • Tumor-infiltrating lymphocytes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Reversed CD4/CD8 ratios of tumor-infiltrating lymphocytes are correlated with the progression of human cervical carcinoma. / Sheu, Bor Ching; Hsu, Su Ming; Ho, Hong Nerng; Lin, Rong Hwa; Torng, Pao Ling; Huang, Su Cheng.

In: Cancer, Vol. 86, No. 8, 15.10.1999, p. 1537-1543.

Research output: Contribution to journalArticle

Sheu, Bor Ching ; Hsu, Su Ming ; Ho, Hong Nerng ; Lin, Rong Hwa ; Torng, Pao Ling ; Huang, Su Cheng. / Reversed CD4/CD8 ratios of tumor-infiltrating lymphocytes are correlated with the progression of human cervical carcinoma. In: Cancer. 1999 ; Vol. 86, No. 8. pp. 1537-1543.
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abstract = "BACKGROUND. To investigate the clinical significance of tumor- infiltrating lymphocytes (TILs)within the tumor milieu of human cervical carcinoma, the authors quantitatively measured and compared the subpopulations of lymphocytes infiltrating the neoplastic cervix. METHODS. A total of 30 patients with Stage Ia-IIa cervical carcinoma were enrolled. TILs were isolated from tissue specimens by means of a mechanical dispersal technique, and the immunocyte subsets were quantified with dual-color flow cytometry. Bulky tumor was defined as tumor size >4 cm in greatest dimension according to the 1995 staging of the International Federation of Gynecology and Obstetrics. RESULTS. The CD4/CD8 ratios of TILs were reversed in both cervical squamous cell carcinoma in = 20) and cervical adenocarcinoma in = 10). The proportion of CD4+ T cells was significantly lower in tumors from patients with lymph node metastasis (n = 8) than in those from patients without lymph node metastasis in = 22) (24.5 vs. 32.7, P = 0.001), as was the reversed CD4/CD8 ratio (0.50 vs. 0.81, P = 0.001). The proportion of CD4+ T cells was much lower in bulky tumors (n = 5) than in nonbulky tumors in = 25) (21.4 vs. 32.5, P < 0 001), reflecting in a more strongly reversed CD4/CD8 ratio (0.41 vs. 0.81, P = 0.001). CONCLUSIONS. Decreased proportions of tumor-infiltrating CD4+ T cells with reversed CD4/CD8 ratios are highly correlated with rapid tumor growth and lymph node metastasis in cervical carcinoma. The regional immune escape is of prognostic importance with regard to cancer progression.",
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T1 - Reversed CD4/CD8 ratios of tumor-infiltrating lymphocytes are correlated with the progression of human cervical carcinoma

AU - Sheu, Bor Ching

AU - Hsu, Su Ming

AU - Ho, Hong Nerng

AU - Lin, Rong Hwa

AU - Torng, Pao Ling

AU - Huang, Su Cheng

PY - 1999/10/15

Y1 - 1999/10/15

N2 - BACKGROUND. To investigate the clinical significance of tumor- infiltrating lymphocytes (TILs)within the tumor milieu of human cervical carcinoma, the authors quantitatively measured and compared the subpopulations of lymphocytes infiltrating the neoplastic cervix. METHODS. A total of 30 patients with Stage Ia-IIa cervical carcinoma were enrolled. TILs were isolated from tissue specimens by means of a mechanical dispersal technique, and the immunocyte subsets were quantified with dual-color flow cytometry. Bulky tumor was defined as tumor size >4 cm in greatest dimension according to the 1995 staging of the International Federation of Gynecology and Obstetrics. RESULTS. The CD4/CD8 ratios of TILs were reversed in both cervical squamous cell carcinoma in = 20) and cervical adenocarcinoma in = 10). The proportion of CD4+ T cells was significantly lower in tumors from patients with lymph node metastasis (n = 8) than in those from patients without lymph node metastasis in = 22) (24.5 vs. 32.7, P = 0.001), as was the reversed CD4/CD8 ratio (0.50 vs. 0.81, P = 0.001). The proportion of CD4+ T cells was much lower in bulky tumors (n = 5) than in nonbulky tumors in = 25) (21.4 vs. 32.5, P < 0 001), reflecting in a more strongly reversed CD4/CD8 ratio (0.41 vs. 0.81, P = 0.001). CONCLUSIONS. Decreased proportions of tumor-infiltrating CD4+ T cells with reversed CD4/CD8 ratios are highly correlated with rapid tumor growth and lymph node metastasis in cervical carcinoma. The regional immune escape is of prognostic importance with regard to cancer progression.

AB - BACKGROUND. To investigate the clinical significance of tumor- infiltrating lymphocytes (TILs)within the tumor milieu of human cervical carcinoma, the authors quantitatively measured and compared the subpopulations of lymphocytes infiltrating the neoplastic cervix. METHODS. A total of 30 patients with Stage Ia-IIa cervical carcinoma were enrolled. TILs were isolated from tissue specimens by means of a mechanical dispersal technique, and the immunocyte subsets were quantified with dual-color flow cytometry. Bulky tumor was defined as tumor size >4 cm in greatest dimension according to the 1995 staging of the International Federation of Gynecology and Obstetrics. RESULTS. The CD4/CD8 ratios of TILs were reversed in both cervical squamous cell carcinoma in = 20) and cervical adenocarcinoma in = 10). The proportion of CD4+ T cells was significantly lower in tumors from patients with lymph node metastasis (n = 8) than in those from patients without lymph node metastasis in = 22) (24.5 vs. 32.7, P = 0.001), as was the reversed CD4/CD8 ratio (0.50 vs. 0.81, P = 0.001). The proportion of CD4+ T cells was much lower in bulky tumors (n = 5) than in nonbulky tumors in = 25) (21.4 vs. 32.5, P < 0 001), reflecting in a more strongly reversed CD4/CD8 ratio (0.41 vs. 0.81, P = 0.001). CONCLUSIONS. Decreased proportions of tumor-infiltrating CD4+ T cells with reversed CD4/CD8 ratios are highly correlated with rapid tumor growth and lymph node metastasis in cervical carcinoma. The regional immune escape is of prognostic importance with regard to cancer progression.

KW - CD4/CD8 ratio

KW - Cervical carcinoma

KW - Lymph node metastasis

KW - Tumor-infiltrating lymphocytes

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