Retrovirus Insertion into Herpesvirus

Characterization of a Marek’s Disease Virus Harboring a Solo LTR

Rhonda Kost, Dan Jones, Robert Isfort, Richard Witter, Hsing Jien Kung

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We recently reported the phenomenon of retroviral insertion into a herpesvirus (Isfort et al., (1992) Proc. Natl. Acad. Sci. USA 89, 991-995). We have now isolated Marek’s disease virus (MDV) clones that carry retroviral inserts. Retroviral LTR insertion has the potential to activate or inactivate herpesvirus genes leading to alterations of the biological properties of the herpesviruses. The structure and the expression pattern of a virus clone carrying a solitary LTR insertion was characterized in detail. The LTR insertion is accompanied by deletions in the herpesvirus genome at each of the junctions of the short unique region (Us) and its inverted repeats (Rs). This results in a significantly truncated Rs region. Despite these structural rearrangements, the virus is replication-competent and demonstrates an enhanced growth rate in vitro. While the MDV homologs of herpes simplex Us genes are retained in this virus, at least two open reading frames are deleted and one transcript observed in wild-type virus is disrupted. In addition to defining several regions of MDV that are nonessential for in vitro growth, this clone illustrates one way in which retroviral information can be stably transmitted by a herpesvirus.

Original languageEnglish
Pages (from-to)161-169
Number of pages9
JournalVirology
Volume192
Issue number1
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

Fingerprint

Marek Disease
Herpesviridae
Retroviridae
Viruses
Clone Cells
Simplexvirus
Virus Replication
Growth
Open Reading Frames
Genes
Genome

ASJC Scopus subject areas

  • Virology

Cite this

Retrovirus Insertion into Herpesvirus : Characterization of a Marek’s Disease Virus Harboring a Solo LTR. / Kost, Rhonda; Jones, Dan; Isfort, Robert; Witter, Richard; Kung, Hsing Jien.

In: Virology, Vol. 192, No. 1, 01.01.1993, p. 161-169.

Research output: Contribution to journalArticle

Kost, Rhonda ; Jones, Dan ; Isfort, Robert ; Witter, Richard ; Kung, Hsing Jien. / Retrovirus Insertion into Herpesvirus : Characterization of a Marek’s Disease Virus Harboring a Solo LTR. In: Virology. 1993 ; Vol. 192, No. 1. pp. 161-169.
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