Retroviral insertional activation in a herpesvirus: Transcriptional activation of US genes by an integrated long terminal repeat in a Marek's disease virus clone

Dan Jones, Peter Brunovskis, Richard Witter, Hsing Jien Kung

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Insertional activation of host proto-oncogenes has been recognized as a basic mechanism by which nonacute retroviruses induce cancer. Our previous work has demonstrated that retroviruses can efficiently integrate into DNA virus genomes. Specifically, coinfection of cultured fibroblasts with a chicken herpesvirus, Marek's disease virus (MDV), and a chicken retrovirus results in frequent stable retroviral insertions into the herpesvirus genome. Such insertions could alter the expression of herpesvirus genes, possibly resulting in novel phenotypic properties. In this article, we report the characterization of a replication-competent clone of MDV with integrated retroviral sequences. This virus was isolated from a chicken following injection of fibroblasts coinfected with MDV and the retrovirus, reticuloendotheliosis virus. Transcripts originating from the reticuloendotheliosis virus long terminal repeat promoters were found to encode the adjoining MDV genes, SORF2, US1, and US10. This virus replicates well in culture but has an unusual phenotype in chickens, characterized by an attenuated virulence which produces no nerve lesions but, rather, severe thymic atrophy. While the causal relationship between the insertion and the observed phenotypes remains to be established, our data provide the first evidence of retroviral insertional activation of herpesvirus genes.

Original languageEnglish
Pages (from-to)2460-2467
Number of pages8
JournalJournal of Virology
Volume70
Issue number4
Publication statusPublished - Dec 1 1996
Externally publishedYes

Fingerprint

Retroviridae
Marek Disease
Mardivirus
terminal repeat sequences
Terminal Repeat Sequences
Insertional Mutagenesis
Herpesviridae
transcriptional activation
Transcriptional Activation
Clone Cells
Reticuloendotheliosis virus
clones
chickens
Viruses
Chickens
Genes
fibroblasts
genes
proto-oncogenes
phenotype

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Retroviral insertional activation in a herpesvirus : Transcriptional activation of US genes by an integrated long terminal repeat in a Marek's disease virus clone. / Jones, Dan; Brunovskis, Peter; Witter, Richard; Kung, Hsing Jien.

In: Journal of Virology, Vol. 70, No. 4, 01.12.1996, p. 2460-2467.

Research output: Contribution to journalArticle

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