Retrospective serological study on sequential dengue virus serotypes 1 to 4 epidemics in Tainan City, Taiwan, 1994 to 2000

Shu Fen Chang, Jyh Hsiung Huang, Li Kuang Chen, Chien Ling Su, Tsai Ling Liao, Li Jung Chien, Ting Hsiang Lin, Chun Jen Su, Pei Yun Shu

Research output: Contribution to journalArticle

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Abstract

Background and Purpose: We previously reported the development of a non-structural protein NS1 serotype-specific immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) for dengue serodiagnosis and seroepidemiological study. This assay can be used to differentiate the immunologic status of individuals into naive, primary, or secondary dengue virus (DENV) infection and identify the DENV serotypes of primary infection. A retrospective study was conducted to investigate the serological responses of confirmed dengue cases infected during each of the sequential DENV-1 (August 1994 to February 1995), DENV-2 (August to December 1997), DENV-3 (August 1998 to January 1999), and DENV-4 (June to December 2000) epidemics in Tainan City, Taiwan. Methods: 218 serum samples collected 1.1 to 7.2 years postinfection were analyzed by NS1 serotype-specific IgG ELISA together with corresponding acute and/or convalescent serum samples when available. The immunological status and the infecting DENV serotypes were determined for these individuals. Results: High titers of dengue NS1 serotype-specific IgG antibody could be detected in serum samples. Differentiation of immunological status showed that 76.6% and 23.4% of cases had primary and secondary infections, respectively. A significant age-dependent increase in the rate of secondary infection was observed for those cases born before 1942. Notably, analysis of postinfection serum samples of 17 dengue hemorrhagic fever patients infected during the 1998 DENV-3 epidemic showed that 9 cases (53%) had primary infections. Conclusions: Our data revealed that a majority of the population born after 1943 in Tainan City are naive to DENV infection and are at high risk of infection with all 4 DENV serotypes.

Original languageEnglish
Pages (from-to)377-385
Number of pages9
JournalJournal of Microbiology, Immunology and Infection
Volume41
Issue number5
Publication statusPublished - Oct 1 2008
Externally publishedYes

Fingerprint

Dengue Virus
Taiwan
Retrospective Studies
Dengue
Immunoglobulin G
Virus Diseases
Serum
Coinfection
Infection
Enzyme-Linked Immunosorbent Assay
Serogroup
Severe Dengue
Seroepidemiologic Studies
Serologic Tests

Keywords

  • Dengue virus
  • Disease outbreaks
  • Enzyme-linked immunosorbent assay
  • Serologic tests
  • Viral nonstructural proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Chang, S. F., Huang, J. H., Chen, L. K., Su, C. L., Liao, T. L., Chien, L. J., ... Shu, P. Y. (2008). Retrospective serological study on sequential dengue virus serotypes 1 to 4 epidemics in Tainan City, Taiwan, 1994 to 2000. Journal of Microbiology, Immunology and Infection, 41(5), 377-385.

Retrospective serological study on sequential dengue virus serotypes 1 to 4 epidemics in Tainan City, Taiwan, 1994 to 2000. / Chang, Shu Fen; Huang, Jyh Hsiung; Chen, Li Kuang; Su, Chien Ling; Liao, Tsai Ling; Chien, Li Jung; Lin, Ting Hsiang; Su, Chun Jen; Shu, Pei Yun.

In: Journal of Microbiology, Immunology and Infection, Vol. 41, No. 5, 01.10.2008, p. 377-385.

Research output: Contribution to journalArticle

Chang, SF, Huang, JH, Chen, LK, Su, CL, Liao, TL, Chien, LJ, Lin, TH, Su, CJ & Shu, PY 2008, 'Retrospective serological study on sequential dengue virus serotypes 1 to 4 epidemics in Tainan City, Taiwan, 1994 to 2000', Journal of Microbiology, Immunology and Infection, vol. 41, no. 5, pp. 377-385.
Chang, Shu Fen ; Huang, Jyh Hsiung ; Chen, Li Kuang ; Su, Chien Ling ; Liao, Tsai Ling ; Chien, Li Jung ; Lin, Ting Hsiang ; Su, Chun Jen ; Shu, Pei Yun. / Retrospective serological study on sequential dengue virus serotypes 1 to 4 epidemics in Tainan City, Taiwan, 1994 to 2000. In: Journal of Microbiology, Immunology and Infection. 2008 ; Vol. 41, No. 5. pp. 377-385.
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abstract = "Background and Purpose: We previously reported the development of a non-structural protein NS1 serotype-specific immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) for dengue serodiagnosis and seroepidemiological study. This assay can be used to differentiate the immunologic status of individuals into naive, primary, or secondary dengue virus (DENV) infection and identify the DENV serotypes of primary infection. A retrospective study was conducted to investigate the serological responses of confirmed dengue cases infected during each of the sequential DENV-1 (August 1994 to February 1995), DENV-2 (August to December 1997), DENV-3 (August 1998 to January 1999), and DENV-4 (June to December 2000) epidemics in Tainan City, Taiwan. Methods: 218 serum samples collected 1.1 to 7.2 years postinfection were analyzed by NS1 serotype-specific IgG ELISA together with corresponding acute and/or convalescent serum samples when available. The immunological status and the infecting DENV serotypes were determined for these individuals. Results: High titers of dengue NS1 serotype-specific IgG antibody could be detected in serum samples. Differentiation of immunological status showed that 76.6{\%} and 23.4{\%} of cases had primary and secondary infections, respectively. A significant age-dependent increase in the rate of secondary infection was observed for those cases born before 1942. Notably, analysis of postinfection serum samples of 17 dengue hemorrhagic fever patients infected during the 1998 DENV-3 epidemic showed that 9 cases (53{\%}) had primary infections. Conclusions: Our data revealed that a majority of the population born after 1943 in Tainan City are naive to DENV infection and are at high risk of infection with all 4 DENV serotypes.",
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AU - Liao, Tsai Ling

AU - Chien, Li Jung

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