Retinoic acid fails to reverse oligohydramnios-induced pulmonary hypoplasia in fetal rats

Chung Ming Chen, Hsiu Chu Chou, Leng-Fang Wang, Yaw Dong Lang, Ching-Ying Yeh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

All-trans retinoic acid (ATRA) stimulates platelet-derived growth factor (PDGF)-A expression and enhances alveolarization in rat lungs. On d 16 of gestation, pregnant Sprague-Dawley rats were randomly assigned to either a retinoic acid group (intragastric ATRA at 10 mg/kg body weight) or a vehicle group. We punctured each amniotic sac, and fetuses in the opposite uterine horn served as controls. On d 21 of gestation, the fetuses were delivered by cesarean section. Rats subjected to oligohydramnios exhibited significantly lower lung weights and lung/body weight ratios, and ATRA had no effects on the body or lung weights of oligohydramnios-exposed rats. Lung PDGF-A and -B mRNA expression was significantly lower in oligohydramnios-exposed rats compared with control littermates of maternal vehicle-treated dams. Maternal retinoic acid treatment significantly increased PDGF-A and -B mRNA expression in control and oligohydramnios-exposed rats compared with all rats and oligohydramnios-exposed rats of maternal vehicle-treated dams, respectively. Rats exposed to oligohydramnios exhibited a significantly lower generation of alveolar saccules than did control rats in the maternal retinoic acid- and vehicle-treated groups. In this model, maternal retinoic acid treatment showed no positive effects on oligohydramnios-induced pulmonary hypoplasia in the pseudoglandular stage.

Original languageEnglish
Pages (from-to)553-558
Number of pages6
JournalPediatric Research
Volume62
Issue number5
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Oligohydramnios
Tretinoin
Lung
Mothers
Proto-Oncogene Proteins c-sis
Fetus
Body Weight
Saccule and Utricle
Weights and Measures
Pregnancy
Messenger RNA
Cesarean Section
Sprague Dawley Rats

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Retinoic acid fails to reverse oligohydramnios-induced pulmonary hypoplasia in fetal rats. / Chen, Chung Ming; Chou, Hsiu Chu; Wang, Leng-Fang; Lang, Yaw Dong; Yeh, Ching-Ying.

In: Pediatric Research, Vol. 62, No. 5, 11.2007, p. 553-558.

Research output: Contribution to journalArticle

Chen, Chung Ming ; Chou, Hsiu Chu ; Wang, Leng-Fang ; Lang, Yaw Dong ; Yeh, Ching-Ying. / Retinoic acid fails to reverse oligohydramnios-induced pulmonary hypoplasia in fetal rats. In: Pediatric Research. 2007 ; Vol. 62, No. 5. pp. 553-558.
@article{32e8bfc8cd354ac78ea8889ac96e133f,
title = "Retinoic acid fails to reverse oligohydramnios-induced pulmonary hypoplasia in fetal rats",
abstract = "All-trans retinoic acid (ATRA) stimulates platelet-derived growth factor (PDGF)-A expression and enhances alveolarization in rat lungs. On d 16 of gestation, pregnant Sprague-Dawley rats were randomly assigned to either a retinoic acid group (intragastric ATRA at 10 mg/kg body weight) or a vehicle group. We punctured each amniotic sac, and fetuses in the opposite uterine horn served as controls. On d 21 of gestation, the fetuses were delivered by cesarean section. Rats subjected to oligohydramnios exhibited significantly lower lung weights and lung/body weight ratios, and ATRA had no effects on the body or lung weights of oligohydramnios-exposed rats. Lung PDGF-A and -B mRNA expression was significantly lower in oligohydramnios-exposed rats compared with control littermates of maternal vehicle-treated dams. Maternal retinoic acid treatment significantly increased PDGF-A and -B mRNA expression in control and oligohydramnios-exposed rats compared with all rats and oligohydramnios-exposed rats of maternal vehicle-treated dams, respectively. Rats exposed to oligohydramnios exhibited a significantly lower generation of alveolar saccules than did control rats in the maternal retinoic acid- and vehicle-treated groups. In this model, maternal retinoic acid treatment showed no positive effects on oligohydramnios-induced pulmonary hypoplasia in the pseudoglandular stage.",
author = "Chen, {Chung Ming} and Chou, {Hsiu Chu} and Leng-Fang Wang and Lang, {Yaw Dong} and Ching-Ying Yeh",
year = "2007",
month = "11",
doi = "10.1203/PDR.0b013e31815685b9",
language = "English",
volume = "62",
pages = "553--558",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Retinoic acid fails to reverse oligohydramnios-induced pulmonary hypoplasia in fetal rats

AU - Chen, Chung Ming

AU - Chou, Hsiu Chu

AU - Wang, Leng-Fang

AU - Lang, Yaw Dong

AU - Yeh, Ching-Ying

PY - 2007/11

Y1 - 2007/11

N2 - All-trans retinoic acid (ATRA) stimulates platelet-derived growth factor (PDGF)-A expression and enhances alveolarization in rat lungs. On d 16 of gestation, pregnant Sprague-Dawley rats were randomly assigned to either a retinoic acid group (intragastric ATRA at 10 mg/kg body weight) or a vehicle group. We punctured each amniotic sac, and fetuses in the opposite uterine horn served as controls. On d 21 of gestation, the fetuses were delivered by cesarean section. Rats subjected to oligohydramnios exhibited significantly lower lung weights and lung/body weight ratios, and ATRA had no effects on the body or lung weights of oligohydramnios-exposed rats. Lung PDGF-A and -B mRNA expression was significantly lower in oligohydramnios-exposed rats compared with control littermates of maternal vehicle-treated dams. Maternal retinoic acid treatment significantly increased PDGF-A and -B mRNA expression in control and oligohydramnios-exposed rats compared with all rats and oligohydramnios-exposed rats of maternal vehicle-treated dams, respectively. Rats exposed to oligohydramnios exhibited a significantly lower generation of alveolar saccules than did control rats in the maternal retinoic acid- and vehicle-treated groups. In this model, maternal retinoic acid treatment showed no positive effects on oligohydramnios-induced pulmonary hypoplasia in the pseudoglandular stage.

AB - All-trans retinoic acid (ATRA) stimulates platelet-derived growth factor (PDGF)-A expression and enhances alveolarization in rat lungs. On d 16 of gestation, pregnant Sprague-Dawley rats were randomly assigned to either a retinoic acid group (intragastric ATRA at 10 mg/kg body weight) or a vehicle group. We punctured each amniotic sac, and fetuses in the opposite uterine horn served as controls. On d 21 of gestation, the fetuses were delivered by cesarean section. Rats subjected to oligohydramnios exhibited significantly lower lung weights and lung/body weight ratios, and ATRA had no effects on the body or lung weights of oligohydramnios-exposed rats. Lung PDGF-A and -B mRNA expression was significantly lower in oligohydramnios-exposed rats compared with control littermates of maternal vehicle-treated dams. Maternal retinoic acid treatment significantly increased PDGF-A and -B mRNA expression in control and oligohydramnios-exposed rats compared with all rats and oligohydramnios-exposed rats of maternal vehicle-treated dams, respectively. Rats exposed to oligohydramnios exhibited a significantly lower generation of alveolar saccules than did control rats in the maternal retinoic acid- and vehicle-treated groups. In this model, maternal retinoic acid treatment showed no positive effects on oligohydramnios-induced pulmonary hypoplasia in the pseudoglandular stage.

UR - http://www.scopus.com/inward/record.url?scp=38849095954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38849095954&partnerID=8YFLogxK

U2 - 10.1203/PDR.0b013e31815685b9

DO - 10.1203/PDR.0b013e31815685b9

M3 - Article

C2 - 18049370

AN - SCOPUS:38849095954

VL - 62

SP - 553

EP - 558

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 5

ER -