Abstract
All-trans retinoic acid (ATRA) stimulates platelet-derived growth factor (PDGF)-A expression and enhances alveolarization in rat lungs. On d 16 of gestation, pregnant Sprague-Dawley rats were randomly assigned to either a retinoic acid group (intragastric ATRA at 10 mg/kg body weight) or a vehicle group. We punctured each amniotic sac, and fetuses in the opposite uterine horn served as controls. On d 21 of gestation, the fetuses were delivered by cesarean section. Rats subjected to oligohydramnios exhibited significantly lower lung weights and lung/body weight ratios, and ATRA had no effects on the body or lung weights of oligohydramnios-exposed rats. Lung PDGF-A and -B mRNA expression was significantly lower in oligohydramnios-exposed rats compared with control littermates of maternal vehicle-treated dams. Maternal retinoic acid treatment significantly increased PDGF-A and -B mRNA expression in control and oligohydramnios-exposed rats compared with all rats and oligohydramnios-exposed rats of maternal vehicle-treated dams, respectively. Rats exposed to oligohydramnios exhibited a significantly lower generation of alveolar saccules than did control rats in the maternal retinoic acid- and vehicle-treated groups. In this model, maternal retinoic acid treatment showed no positive effects on oligohydramnios-induced pulmonary hypoplasia in the pseudoglandular stage.
| Original language | English |
|---|---|
| Pages (from-to) | 553-558 |
| Number of pages | 6 |
| Journal | Pediatric Research |
| Volume | 62 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Nov 2007 |
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ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
Cite this
Retinoic acid fails to reverse oligohydramnios-induced pulmonary hypoplasia in fetal rats. / Chen, Chung Ming; Chou, Hsiu Chu; Wang, Leng-Fang; Lang, Yaw Dong; Yeh, Ching-Ying.
In: Pediatric Research, Vol. 62, No. 5, 11.2007, p. 553-558.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Retinoic acid fails to reverse oligohydramnios-induced pulmonary hypoplasia in fetal rats
AU - Chen, Chung Ming
AU - Chou, Hsiu Chu
AU - Wang, Leng-Fang
AU - Lang, Yaw Dong
AU - Yeh, Ching-Ying
PY - 2007/11
Y1 - 2007/11
N2 - All-trans retinoic acid (ATRA) stimulates platelet-derived growth factor (PDGF)-A expression and enhances alveolarization in rat lungs. On d 16 of gestation, pregnant Sprague-Dawley rats were randomly assigned to either a retinoic acid group (intragastric ATRA at 10 mg/kg body weight) or a vehicle group. We punctured each amniotic sac, and fetuses in the opposite uterine horn served as controls. On d 21 of gestation, the fetuses were delivered by cesarean section. Rats subjected to oligohydramnios exhibited significantly lower lung weights and lung/body weight ratios, and ATRA had no effects on the body or lung weights of oligohydramnios-exposed rats. Lung PDGF-A and -B mRNA expression was significantly lower in oligohydramnios-exposed rats compared with control littermates of maternal vehicle-treated dams. Maternal retinoic acid treatment significantly increased PDGF-A and -B mRNA expression in control and oligohydramnios-exposed rats compared with all rats and oligohydramnios-exposed rats of maternal vehicle-treated dams, respectively. Rats exposed to oligohydramnios exhibited a significantly lower generation of alveolar saccules than did control rats in the maternal retinoic acid- and vehicle-treated groups. In this model, maternal retinoic acid treatment showed no positive effects on oligohydramnios-induced pulmonary hypoplasia in the pseudoglandular stage.
AB - All-trans retinoic acid (ATRA) stimulates platelet-derived growth factor (PDGF)-A expression and enhances alveolarization in rat lungs. On d 16 of gestation, pregnant Sprague-Dawley rats were randomly assigned to either a retinoic acid group (intragastric ATRA at 10 mg/kg body weight) or a vehicle group. We punctured each amniotic sac, and fetuses in the opposite uterine horn served as controls. On d 21 of gestation, the fetuses were delivered by cesarean section. Rats subjected to oligohydramnios exhibited significantly lower lung weights and lung/body weight ratios, and ATRA had no effects on the body or lung weights of oligohydramnios-exposed rats. Lung PDGF-A and -B mRNA expression was significantly lower in oligohydramnios-exposed rats compared with control littermates of maternal vehicle-treated dams. Maternal retinoic acid treatment significantly increased PDGF-A and -B mRNA expression in control and oligohydramnios-exposed rats compared with all rats and oligohydramnios-exposed rats of maternal vehicle-treated dams, respectively. Rats exposed to oligohydramnios exhibited a significantly lower generation of alveolar saccules than did control rats in the maternal retinoic acid- and vehicle-treated groups. In this model, maternal retinoic acid treatment showed no positive effects on oligohydramnios-induced pulmonary hypoplasia in the pseudoglandular stage.
UR - http://www.scopus.com/inward/record.url?scp=38849095954&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38849095954&partnerID=8YFLogxK
U2 - 10.1203/PDR.0b013e31815685b9
DO - 10.1203/PDR.0b013e31815685b9
M3 - Article
C2 - 18049370
AN - SCOPUS:38849095954
VL - 62
SP - 553
EP - 558
JO - Pediatric Research
JF - Pediatric Research
SN - 0031-3998
IS - 5
ER -