Resveratrol Suppresses the Angiogenesis and Tumor Growth of Gliomas in Rats

Sheng Hong Tseng, Swei Ming Lin, Jin Cherng Chen, Yen Hao Su, Hsin Yi Huang, Chia Kang Chen, Po Yin Lin, Yun Chen

Research output: Contribution to journalArticle

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Abstract

Purpose: We wanted to investigate the antitumor effects and effect on angiogenesis of resveratrol in rat RT-2 gliomas. Experimental Design: RT-2 glioma cells were treated with resveratrol, and then cytotoxicity was assayed, apoptosis was measured by flow-activated cell sorter flow cytometry, and expression of vascular endothelial growth factor was measured by reverse transcription-PCR. Tumor size, animal survival time, and survival rate were followed in resveratrol-treated rats with s.c. or intracerebral gliomas. Furthermore, in vitro proliferation was assayed to explore the effect of resveratrol on the proliferation of ECV304 human umbilical vein endothelial cells. Expression of CD31 in resveratrol-treated gliomas was followed immunohistochemically to study the effect of resveratrol on the glioma-induced angiogenesis. Results: Resveratrol was demonstrated to exert cytotoxic effects and induce glioma cell apoptosis in a concentration- and time-dependent manner (P < 0.05). Resveratrol (40 mg/kg/day) exerted significant antitumor effects on s.c. tumors, including slower tumor growth rate, longer animal survival time, and higher animal survival rate (P < 0.05). In contrast, resveratrol affected intracerebral tumors at only an increased dose (100 mg/kg/day), prolonging animal survival (P < 0.05) without affecting survival rate. The expression of vascular endothelial growth factor in the glioma cells and the proliferation of ECV304 cells were inhibited by resveratrol in a concentration-dependent manner. Immunohistochemical analyses showed that the s.c. gliomas from resveratrol-treated rats had fewer microvessel densities than did control rats (P < 0.01). Conclusions: Resveratrol caused significant glioma cell cytotoxicity and apoptosis, exerted antitumor effects on the s.c. and intracerebral gliomas, and inhibited angiogenesis in s.c. gliomas. Thus, resveratrol might be considered a possible treatment strategy for gliomas.

Original languageEnglish
Pages (from-to)2190-2202
Number of pages13
JournalClinical Cancer Research
Volume10
Issue number6
DOIs
Publication statusPublished - Mar 15 2004
Externally publishedYes

Fingerprint

Glioma
Growth
Neoplasms
Apoptosis
resveratrol
Vascular Endothelial Growth Factor A
Human Umbilical Vein Endothelial Cells
Microvessels
Reverse Transcription
Flow Cytometry
Research Design
Cell Proliferation
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Tseng, S. H., Lin, S. M., Chen, J. C., Su, Y. H., Huang, H. Y., Chen, C. K., ... Chen, Y. (2004). Resveratrol Suppresses the Angiogenesis and Tumor Growth of Gliomas in Rats. Clinical Cancer Research, 10(6), 2190-2202. https://doi.org/10.1158/1078-0432.CCR-03-0105

Resveratrol Suppresses the Angiogenesis and Tumor Growth of Gliomas in Rats. / Tseng, Sheng Hong; Lin, Swei Ming; Chen, Jin Cherng; Su, Yen Hao; Huang, Hsin Yi; Chen, Chia Kang; Lin, Po Yin; Chen, Yun.

In: Clinical Cancer Research, Vol. 10, No. 6, 15.03.2004, p. 2190-2202.

Research output: Contribution to journalArticle

Tseng, SH, Lin, SM, Chen, JC, Su, YH, Huang, HY, Chen, CK, Lin, PY & Chen, Y 2004, 'Resveratrol Suppresses the Angiogenesis and Tumor Growth of Gliomas in Rats', Clinical Cancer Research, vol. 10, no. 6, pp. 2190-2202. https://doi.org/10.1158/1078-0432.CCR-03-0105
Tseng, Sheng Hong ; Lin, Swei Ming ; Chen, Jin Cherng ; Su, Yen Hao ; Huang, Hsin Yi ; Chen, Chia Kang ; Lin, Po Yin ; Chen, Yun. / Resveratrol Suppresses the Angiogenesis and Tumor Growth of Gliomas in Rats. In: Clinical Cancer Research. 2004 ; Vol. 10, No. 6. pp. 2190-2202.
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AU - Su, Yen Hao

AU - Huang, Hsin Yi

AU - Chen, Chia Kang

AU - Lin, Po Yin

AU - Chen, Yun

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