Resveratrol suppresses calcium-mediated microglial activation and rescues hippocampal neurons of adult rats following acute bacterial meningitis

Ji Nan Sheu, Wen Chieh Liao, Un In Wu, Ling Yuh Shyu, Fu-Der Mai, Li You Chen, Mei Jung Chen, Su Chung Youn, Hung-Ming Chang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Acute bacterial meningitis (ABM) is a serious disease with severe neurological sequelae. The intense calcium-mediated microglial activation and subsequently pro-inflammatory cytokine release plays an important role in eliciting ABM-related oxidative damage. Considering resveratrol possesses significant anti-inflammatory and anti-oxidative properties, the present study aims to determine whether resveratrol would exert beneficial effects on hippocampal neurons following ABM. ABM was induced by inoculating Klebsiella pneumoniae into adult rats intraventricularly. The time-of-flight secondary ion mass spectrometry (TOF-SIMS), Griffonia simplicifolia isolectin-B4 (GSA-IB4) and ionized calcium binding adaptor molecule 1 (Iba1) immunohistochemistry, enzyme-linked immunosorbent assay as well as malondialdehyde (MDA) measurement were used to examine the calcium expression, microglial activation, pro-inflammatory cytokine level, and extent of oxidative stress, respectively. In ABM rats, strong calcium signaling associated with enhanced microglial activation was observed in hippocampus. Increased microglial expression was coincided with intense production of pro-inflammatory cytokines and oxidative damage. However, in rats receiving resveratrol after ABM, the calcium intensity, microglial activation, pro-inflammatory cytokine and MDA levels were all significantly decreased. Quantitative data showed that much more hippocampal neurons were survived in resveratrol-treated rats following ABM. As resveratrol successfully rescues hippocampal neurons from ABM by suppressing the calcium-mediated microglial activation, therapeutic use of resveratrol may act as a promising strategy to counteract the ABM-induced neurological damage.

Original languageEnglish
Pages (from-to)137-148
Number of pages12
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume36
Issue number2
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Bacterial Meningitides
meningitis
resveratrol
neurons
Calcium
Neurons
calcium
rats
cytokines
Cytokines
Malondialdehyde
malondialdehyde
Griffonia simplicifolia
Secondary Ion Mass Spectrometry
Calcium Signaling
complications (disease)
Klebsiella pneumoniae
Therapeutic Uses
hippocampus
immunohistochemistry

Keywords

  • Klebsiella pneumoniae
  • Microglia
  • Oxidative stress
  • Pro-inflammatory cytokines
  • Resveratrol
  • TOF-SIMS

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases
  • Immunology and Allergy
  • veterinary(all)

Cite this

Resveratrol suppresses calcium-mediated microglial activation and rescues hippocampal neurons of adult rats following acute bacterial meningitis. / Sheu, Ji Nan; Liao, Wen Chieh; Wu, Un In; Shyu, Ling Yuh; Mai, Fu-Der; Chen, Li You; Chen, Mei Jung; Youn, Su Chung; Chang, Hung-Ming.

In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 36, No. 2, 03.2013, p. 137-148.

Research output: Contribution to journalArticle

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