Resveratrol protects muscle cells against palmitate-induced cellular senescence and insulin resistance through ameliorating autophagic flux

Yun Ching Chang, Hung Wen Liu, Yi Tien Chen, Yun An Chen, Yen Ju Chen, Sue Joan Chang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Skeletal muscle, a highly metabolic tissue, is particularly vulnerable to increased levels of saturated free fatty acids (FFAs). The role of autophagy in saturated FFAs-induced cellular senescence and insulin resistance in skeletal muscle remains unclear. Therefore, the present study was aimed to explore autophagic flux in cellular senescence and insulin resistance induced by palmitate in muscle cells, and whether resveratrol limited these responses. Our results showed that palmitate induced cellular senescence in both myoblasts and myotubes. In addition, palmitate delayed differentiation in myoblasts and inhibited expression of insulin-stimulated p-AKTSer473 in myotubes. The accumulations of autophagosome assessed by tandem fluorescent-tagged LC3 demonstrated that autophagic flux was impaired in both palmitate-treated myoblasts and myotubes. Resveratrol protected muscle cells from palmitate-induced cellular senescence, apoptosis during differentiation, and insulin resistance via ameliorating autophagic flux. The direct influence of autophagic flux on development of cellular senescence and insulin resistance was confirmed by blockage of autophagic flux with chloroquine. In conclusion, impairment of autophagic flux is crucial for palmitate-induced cellular senescence and insulin resistance in muscle cells. Restoring autophagic flux by resveratrol could be a promising approach to prevent cellular senescence and ameliorate insulin resistance in muscle.

Original languageEnglish
JournalJournal of Food and Drug Analysis
DOIs
Publication statusAccepted/In press - Jan 1 2018

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Palmitates
Cell Aging
palmitates
resveratrol
insulin resistance
myocytes
Muscle Cells
Insulin Resistance
myoblasts
Myoblasts
Skeletal Muscle Fibers
Nonesterified Fatty Acids
skeletal muscle
free fatty acids
Fatty Acids
chloroquine
autophagy
Autophagy
Chloroquine
cell senescence

Keywords

  • Autophagic flux
  • Cellular senescence
  • Insulin resistance
  • Resveratrol

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

Resveratrol protects muscle cells against palmitate-induced cellular senescence and insulin resistance through ameliorating autophagic flux. / Chang, Yun Ching; Liu, Hung Wen; Chen, Yi Tien; Chen, Yun An; Chen, Yen Ju; Chang, Sue Joan.

In: Journal of Food and Drug Analysis, 01.01.2018.

Research output: Contribution to journalArticle

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AU - Chen, Yen Ju

AU - Chang, Sue Joan

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