Resveratrol inhibits urban particulate matter-induced COX-2/PGE2 release in human fibroblast-like synoviocytes via the inhibition of activation of NADPH oxidase/ROS/NF-κB

Ming Horng Tsai, Lee Fen Hsu, Chiang Wen Lee, Yao Chang Chiang, Ming Hsueh Lee, Jia Mei How, Ching Mei Wu, Chieh Liang Huang, I-Ta Lee

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Human fibroblast-like synoviocytes (FLSs) play a role in joint synovial inflammation in rheumatoid arthritis (RA). Some evidence indicates that particulate matter (PM) in air pollution could contribute to the progression of RA. However, more research is needed to clarify this relationship. Up-regulation of cyclooxygenase (COX)-2 and its metabolite prostaglandin E2 (PGE2) are implicated in various inflammatory diseases. Resveratrol, a polyphenol found mainly in grapes and red wine, has antioxidant and anti-inflammatory activities. In the present study, we demonstrated that resveratrol reduced PM-induced COX-2/PGE2 expression in human FLSs, and attenuated PM-enhanced NADPH oxidase activity and ROS generation. In addition, PM induced Akt, ERK1/2, or p38 MAPK activation, which was inhibited by resveratrol. Finally, we demonstrated that PM enhanced NF-κB p65 phosphorylation and the NF-κB promoter activity, which were reduced by pretreatment with a ROS inhibitor or resveratrol. Thus, we concluded that resveratrol functions as a suppressor of PM-induced inflammatory signaling pathways by inhibiting COX-2/PGE2 expression.

Original languageEnglish
Pages (from-to)113-123
Number of pages11
JournalInternational Journal of Biochemistry and Cell Biology
Volume88
DOIs
Publication statusPublished - Jul 1 2017

Fingerprint

Particulate Matter
NADPH Oxidase
Cyclooxygenase 2
Fibroblasts
Dinoprostone
Chemical activation
Rheumatoid Arthritis
Phosphorylation
Wine
Air Pollution
Vitis
Polyphenols
p38 Mitogen-Activated Protein Kinases
Metabolites
Air pollution
resveratrol
Synoviocytes
Anti-Inflammatory Agents
Up-Regulation
Antioxidants

Keywords

  • Cyclooxygenase-2
  • Inflammation
  • Particulate matter
  • Prostaglandin E
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Resveratrol inhibits urban particulate matter-induced COX-2/PGE2 release in human fibroblast-like synoviocytes via the inhibition of activation of NADPH oxidase/ROS/NF-κB. / Tsai, Ming Horng; Hsu, Lee Fen; Lee, Chiang Wen; Chiang, Yao Chang; Lee, Ming Hsueh; How, Jia Mei; Wu, Ching Mei; Huang, Chieh Liang; Lee, I-Ta.

In: International Journal of Biochemistry and Cell Biology, Vol. 88, 01.07.2017, p. 113-123.

Research output: Contribution to journalArticle

Tsai, Ming Horng ; Hsu, Lee Fen ; Lee, Chiang Wen ; Chiang, Yao Chang ; Lee, Ming Hsueh ; How, Jia Mei ; Wu, Ching Mei ; Huang, Chieh Liang ; Lee, I-Ta. / Resveratrol inhibits urban particulate matter-induced COX-2/PGE2 release in human fibroblast-like synoviocytes via the inhibition of activation of NADPH oxidase/ROS/NF-κB. In: International Journal of Biochemistry and Cell Biology. 2017 ; Vol. 88. pp. 113-123.
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abstract = "Human fibroblast-like synoviocytes (FLSs) play a role in joint synovial inflammation in rheumatoid arthritis (RA). Some evidence indicates that particulate matter (PM) in air pollution could contribute to the progression of RA. However, more research is needed to clarify this relationship. Up-regulation of cyclooxygenase (COX)-2 and its metabolite prostaglandin E2 (PGE2) are implicated in various inflammatory diseases. Resveratrol, a polyphenol found mainly in grapes and red wine, has antioxidant and anti-inflammatory activities. In the present study, we demonstrated that resveratrol reduced PM-induced COX-2/PGE2 expression in human FLSs, and attenuated PM-enhanced NADPH oxidase activity and ROS generation. In addition, PM induced Akt, ERK1/2, or p38 MAPK activation, which was inhibited by resveratrol. Finally, we demonstrated that PM enhanced NF-κB p65 phosphorylation and the NF-κB promoter activity, which were reduced by pretreatment with a ROS inhibitor or resveratrol. Thus, we concluded that resveratrol functions as a suppressor of PM-induced inflammatory signaling pathways by inhibiting COX-2/PGE2 expression.",
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