Resveratrol inhibits Staphylococcus aureus-induced TLR2/MyD88/NF-κB-dependent VCAM-1 expression in human lung epithelial cells.

I-Ta Lee, Chih Chung Lin, Chih Kai Hsu, Ming Yen Wu, Rou Ling Cho, Chuen Mao Yang

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Abstract

Staphylococcus aureus is the most commonly found Gram-positive bacterium in patients admitted to intensive-care units, causing septicaemia or pneumonia. S. aureus is considered to play an important role in the induction of cell adhesion molecules. Resveratrol, a compound found in the skins of red fruits, may inhibit the inflammatory signalling pathways involved in lung diseases. In the present paper, we have shown that resveratrol reduced S. aureus-mediated VCAM-1 (vascular cell adhesion molecule-1) expression in HPAEpiCs (human lung epithelial cells) and lungs of mice. In an in vivo study, we have shown that resveratrol inhibited S. aureus-induced pulmonary haematoma and leucocyte count in BAL (bronchoalveolar lavage) fluid in mice. In an in vitro study, we observed that resveratrol attenuated S. aureus-induced TLR2 (Toll-like receptor 2), MyD88 (myeloid differentiation factor 88) and PI3K (phosphoinositide 3-kinase) complex formation. S. aureus stimulated Akt, JNK1/2 (c-Jun N-terminal kinase 1/2) and p42/p44 MAPK (mitogen-activated protein kinase) phosphorylation, which were inhibited by resveratrol. In addition, S. aureus induced IκB (inhibitor of nuclear factor κB) α and NF-κB (nuclear factor κB) p65 phosphorylation and NF-κB p65 translocation, which were reduced by resveratrol. Finally, we found that S. aureus induced NF-κB and p300 complex formation and p300 phosphorylation, which were inhibited by resveratrol. Thus resveratrol functions as a suppressor of S. aureus-induced inflammatory signalling not only by inhibiting VCAM-1 expression, but also by reducing TLR2-MyD88-PI3K complex formation and Akt, JNK1/2, p42/p44 MAPK, p300 and NF-κB activation in HPAEpiCs.

Original languageEnglish
Pages (from-to)375-390
Number of pages16
JournalClinical science (London, England : 1979)
Volume127
Issue number6
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Fingerprint

Myeloid Differentiation Factor 88
Toll-Like Receptor 2
Vascular Cell Adhesion Molecule-1
Staphylococcus aureus
Epithelial Cells
Lung
Mitogen-Activated Protein Kinase 9
Mitogen-Activated Protein Kinase 8
1-Phosphatidylinositol 4-Kinase
Mitogen-Activated Protein Kinase 1
Phosphorylation
resveratrol
Bronchoalveolar Lavage Fluid
Cell Adhesion Molecules
Gram-Positive Bacteria
Leukocyte Count
Hematoma
Lung Diseases
Intensive Care Units
Fruit

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Resveratrol inhibits Staphylococcus aureus-induced TLR2/MyD88/NF-κB-dependent VCAM-1 expression in human lung epithelial cells. / Lee, I-Ta; Lin, Chih Chung; Hsu, Chih Kai; Wu, Ming Yen; Cho, Rou Ling; Yang, Chuen Mao.

In: Clinical science (London, England : 1979), Vol. 127, No. 6, 01.01.2014, p. 375-390.

Research output: Contribution to journalArticle

Lee, I-Ta ; Lin, Chih Chung ; Hsu, Chih Kai ; Wu, Ming Yen ; Cho, Rou Ling ; Yang, Chuen Mao. / Resveratrol inhibits Staphylococcus aureus-induced TLR2/MyD88/NF-κB-dependent VCAM-1 expression in human lung epithelial cells. In: Clinical science (London, England : 1979). 2014 ; Vol. 127, No. 6. pp. 375-390.
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