Resveratrol inhibits proliferation of myometrial and leiomyoma cells and decreases extracellular matrix-associated protein expression

Chi Hao Wu, Tzong Ming Shieh, Lin Hung Wei, Ting Fang Cheng, Hsin Yuan Chen, Tsui Chin Huang, Kai Lee Wang, Shih Min Hsia

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Uterine fibroids (leiomyomas) are the most common benign tumours in women during their reproductive age. Hyperplasia of uterine smooth muscle cells and abnormal deposition of extracellular matrix (ECM) are responsible for the development of uterine fibroids. Studies have shown that food rich in phytochemicals can prevent or treat leiomyoma. Therefore, the purpose of this study is to demonstrate the inhibitory effects of resveratrol on uterine fibroid cell growth and ECM-associated protein expression. We found that resveratrol inhibited the proliferation of leiomyoma cell line (ELT3) and uterine smooth muscle cell line (UtSMC) and affected the cell morphology of both cell lines, induced cell cycle arrest at S and G2/M phase, regulated cell apoptosis associated protein expression and induced cell apoptosis, and affected ECM-associated mRNA and protein expression. Our results suggest that resveratrol is potentially effective in preventing the hyperplasia of leiomyoma and uterine smooth muscle cells.

Original languageEnglish
Pages (from-to)241-252
Number of pages12
JournalJournal of Functional Foods
Volume23
DOIs
Publication statusPublished - May 1 2016

Fingerprint

Extracellular Matrix Proteins
resveratrol
Leiomyoma
extracellular matrix
smooth muscle
myocytes
protein synthesis
cell lines
hyperplasia
apoptosis
Myometrium
Smooth Muscle Myocytes
cells
mitosis
phytopharmaceuticals
cell growth
Cell Line
Hyperplasia
neoplasms
Apoptosis

Keywords

  • Apoptosis
  • Cell cycle
  • Extracellular matrix
  • Proliferation
  • Resveratrol
  • Uterine fibroids

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Resveratrol inhibits proliferation of myometrial and leiomyoma cells and decreases extracellular matrix-associated protein expression. / Wu, Chi Hao; Shieh, Tzong Ming; Wei, Lin Hung; Cheng, Ting Fang; Chen, Hsin Yuan; Huang, Tsui Chin; Wang, Kai Lee; Hsia, Shih Min.

In: Journal of Functional Foods, Vol. 23, 01.05.2016, p. 241-252.

Research output: Contribution to journalArticle

Wu, Chi Hao ; Shieh, Tzong Ming ; Wei, Lin Hung ; Cheng, Ting Fang ; Chen, Hsin Yuan ; Huang, Tsui Chin ; Wang, Kai Lee ; Hsia, Shih Min. / Resveratrol inhibits proliferation of myometrial and leiomyoma cells and decreases extracellular matrix-associated protein expression. In: Journal of Functional Foods. 2016 ; Vol. 23. pp. 241-252.
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AU - Wu, Chi Hao

AU - Shieh, Tzong Ming

AU - Wei, Lin Hung

AU - Cheng, Ting Fang

AU - Chen, Hsin Yuan

AU - Huang, Tsui Chin

AU - Wang, Kai Lee

AU - Hsia, Shih Min

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AB - Uterine fibroids (leiomyomas) are the most common benign tumours in women during their reproductive age. Hyperplasia of uterine smooth muscle cells and abnormal deposition of extracellular matrix (ECM) are responsible for the development of uterine fibroids. Studies have shown that food rich in phytochemicals can prevent or treat leiomyoma. Therefore, the purpose of this study is to demonstrate the inhibitory effects of resveratrol on uterine fibroid cell growth and ECM-associated protein expression. We found that resveratrol inhibited the proliferation of leiomyoma cell line (ELT3) and uterine smooth muscle cell line (UtSMC) and affected the cell morphology of both cell lines, induced cell cycle arrest at S and G2/M phase, regulated cell apoptosis associated protein expression and induced cell apoptosis, and affected ECM-associated mRNA and protein expression. Our results suggest that resveratrol is potentially effective in preventing the hyperplasia of leiomyoma and uterine smooth muscle cells.

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