Resveratrol causes COX-2- and p53-dependent apoptosis in head and neck squamous cell cancer cells

Hung Yun Lin, Mingzeng Sun, Heng Yuan Tang, Tessa M. Simone, Yun Hsuan Wu, Jennifer R. Grandis, H. James Cao, Paul J. Davis, Faith B. Davis

Research output: Contribution to journalArticle

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Abstract

Cyclooxygenase-2 (COX-2) content is increased in many types of tumor cells. We have investigated the mechanism by which resveratrol, a stilbene that is pro-apoptotic in many tumor cell lines, causes apoptosis in human head and neck squamous cell carcinoma UMSCC-22B cells by a mechanism involving cellular COX-2. UMSCC-22B cells treated with resveratrol for 24 h, with or without selected inhibitors, were examined: (1) for the presence of nuclear activated ERK1/2, p53 and COX-2, (2) for evidence of apoptosis, and (3) by chromatin immunoprecipitation to demonstrate p53 binding to the p21 promoter. Stilbene-induced apoptosis was concentration-dependent, and associated with ERK1/2 activation, serine-15 p53 phosphorylation and nuclear accumulation of these proteins. These effects were blocked by inhibition of either ERK1/2 or p53 activation. Resveratrol also caused p53 binding to the p21 promoter and increased abundance of COX-2 protein in UMSCC-22B cell nuclei. Resveratrol-induced nuclear COX-2 accumulation was dependent upon ERK1/2 activation, but not p53 activation. Activation of p53 and p53-dependent apoptosis were blocked by the COX-2 inhibitor, NS398, and by transfection of cells with COX-2-siRNA. In UMSCC-22B cells, resveratrol-induced apoptosis and induction of nuclear COX-2 accumulation share dependence on the ERK1/2 signal transduction pathway. Resveratrol-inducible nuclear accumulation of COX-2 is essential for p53 activation and p53-dependent apoptosis in these cancer cells.

Original languageEnglish
Pages (from-to)2131-2142
Number of pages12
JournalJournal of Cellular Biochemistry
Volume104
Issue number6
DOIs
Publication statusPublished - Aug 15 2008
Externally publishedYes

Fingerprint

Squamous Cell Neoplasms
Cyclooxygenase 2
Head and Neck Neoplasms
Head
Cells
Apoptosis
Chemical activation
Stilbenes
Tumors
Signal transduction
Phosphorylation
resveratrol
Epithelial Cells
Cyclooxygenase 2 Inhibitors
Chromatin Immunoprecipitation
Nuclear Proteins
Tumor Cell Line
Cell Nucleus
Serine
Small Interfering RNA

Keywords

  • Apoptosis
  • Cyclooxygenase-2
  • p53
  • Resveratrol
  • UMSCC-22B

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Resveratrol causes COX-2- and p53-dependent apoptosis in head and neck squamous cell cancer cells. / Lin, Hung Yun; Sun, Mingzeng; Tang, Heng Yuan; Simone, Tessa M.; Wu, Yun Hsuan; Grandis, Jennifer R.; Cao, H. James; Davis, Paul J.; Davis, Faith B.

In: Journal of Cellular Biochemistry, Vol. 104, No. 6, 15.08.2008, p. 2131-2142.

Research output: Contribution to journalArticle

Lin, HY, Sun, M, Tang, HY, Simone, TM, Wu, YH, Grandis, JR, Cao, HJ, Davis, PJ & Davis, FB 2008, 'Resveratrol causes COX-2- and p53-dependent apoptosis in head and neck squamous cell cancer cells', Journal of Cellular Biochemistry, vol. 104, no. 6, pp. 2131-2142. https://doi.org/10.1002/jcb.21772
Lin, Hung Yun ; Sun, Mingzeng ; Tang, Heng Yuan ; Simone, Tessa M. ; Wu, Yun Hsuan ; Grandis, Jennifer R. ; Cao, H. James ; Davis, Paul J. ; Davis, Faith B. / Resveratrol causes COX-2- and p53-dependent apoptosis in head and neck squamous cell cancer cells. In: Journal of Cellular Biochemistry. 2008 ; Vol. 104, No. 6. pp. 2131-2142.
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abstract = "Cyclooxygenase-2 (COX-2) content is increased in many types of tumor cells. We have investigated the mechanism by which resveratrol, a stilbene that is pro-apoptotic in many tumor cell lines, causes apoptosis in human head and neck squamous cell carcinoma UMSCC-22B cells by a mechanism involving cellular COX-2. UMSCC-22B cells treated with resveratrol for 24 h, with or without selected inhibitors, were examined: (1) for the presence of nuclear activated ERK1/2, p53 and COX-2, (2) for evidence of apoptosis, and (3) by chromatin immunoprecipitation to demonstrate p53 binding to the p21 promoter. Stilbene-induced apoptosis was concentration-dependent, and associated with ERK1/2 activation, serine-15 p53 phosphorylation and nuclear accumulation of these proteins. These effects were blocked by inhibition of either ERK1/2 or p53 activation. Resveratrol also caused p53 binding to the p21 promoter and increased abundance of COX-2 protein in UMSCC-22B cell nuclei. Resveratrol-induced nuclear COX-2 accumulation was dependent upon ERK1/2 activation, but not p53 activation. Activation of p53 and p53-dependent apoptosis were blocked by the COX-2 inhibitor, NS398, and by transfection of cells with COX-2-siRNA. In UMSCC-22B cells, resveratrol-induced apoptosis and induction of nuclear COX-2 accumulation share dependence on the ERK1/2 signal transduction pathway. Resveratrol-inducible nuclear accumulation of COX-2 is essential for p53 activation and p53-dependent apoptosis in these cancer cells.",
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AU - Sun, Mingzeng

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AU - Wu, Yun Hsuan

AU - Grandis, Jennifer R.

AU - Cao, H. James

AU - Davis, Paul J.

AU - Davis, Faith B.

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N2 - Cyclooxygenase-2 (COX-2) content is increased in many types of tumor cells. We have investigated the mechanism by which resveratrol, a stilbene that is pro-apoptotic in many tumor cell lines, causes apoptosis in human head and neck squamous cell carcinoma UMSCC-22B cells by a mechanism involving cellular COX-2. UMSCC-22B cells treated with resveratrol for 24 h, with or without selected inhibitors, were examined: (1) for the presence of nuclear activated ERK1/2, p53 and COX-2, (2) for evidence of apoptosis, and (3) by chromatin immunoprecipitation to demonstrate p53 binding to the p21 promoter. Stilbene-induced apoptosis was concentration-dependent, and associated with ERK1/2 activation, serine-15 p53 phosphorylation and nuclear accumulation of these proteins. These effects were blocked by inhibition of either ERK1/2 or p53 activation. Resveratrol also caused p53 binding to the p21 promoter and increased abundance of COX-2 protein in UMSCC-22B cell nuclei. Resveratrol-induced nuclear COX-2 accumulation was dependent upon ERK1/2 activation, but not p53 activation. Activation of p53 and p53-dependent apoptosis were blocked by the COX-2 inhibitor, NS398, and by transfection of cells with COX-2-siRNA. In UMSCC-22B cells, resveratrol-induced apoptosis and induction of nuclear COX-2 accumulation share dependence on the ERK1/2 signal transduction pathway. Resveratrol-inducible nuclear accumulation of COX-2 is essential for p53 activation and p53-dependent apoptosis in these cancer cells.

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