Resveratrol arrests cell cycle and induces apoptosis in human hepatocellular carcinoma Huh-7 cells.

Pei Chi Liao, Lean Teik Ng, Liang Tzung Lin, Christopher D. Richardson, Guey Horng Wang, Chun Ching Lin

Research output: Contribution to journalArticle

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Abstract

Resveratrol has been shown to possess anticancer, anti-aging, anti-inflammatory, antimicrobial, and neuroprotective activities. In this study, we examined the antiproliferative properties of resveratrol and its molecular mechanism(s) of action in Huh-7 cells, a new human hepatoma cell line system for hepatitis C virus. Results showed that resveratrol significantly inhibited Huh-7 cell proliferation (50% inhibitory concentration = 22.4 μg/mL) and effectively induced cell cycle arrest and apoptosis. It up-regulated p21/WAF1 expression in a p53-independent manner, but the expressions of cyclin E, cyclin A, and cyclin-dependent kinase 2 were down-regulated. It also caused an increase in the ratio of pro-apoptotic/anti-apoptotic protein, which was associated with the mitochondrial membrane depolarization and the increase in caspase activity. Resveratrol showed no effect on Fas, Fas ligand, extracellular signal regulated kinase (ERK) 1/2, and p38 expression but down-regulated phospho-ERK and phospho-p38 expression. In addition, resveratrol was noted to trigger autophagic cell death through the increased expression of autophagy-related Atg5, Atg7, Atg9, and Atg12 proteins. These results suggest that resveratrol could be an important chemoprevention agent for hepatoma of hepatitis C virus infection.

Original languageEnglish
Pages (from-to)1415-1423
Number of pages9
JournalJournal of Medicinal Food
Volume13
Issue number6
Publication statusPublished - Dec 2010
Externally publishedYes

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Cell Cycle Checkpoints
Hepatocellular Carcinoma
Apoptosis
Autophagy
Hepacivirus
Cyclin-Dependent Kinase 2
Cyclin A
Cyclin E
Apoptosis Regulatory Proteins
Fas Ligand Protein
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Extracellular Signal-Regulated MAP Kinases
Chemoprevention
Mitochondrial Membranes
Virus Diseases
Caspases
Inhibitory Concentration 50
resveratrol
Anti-Inflammatory Agents

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Liao, P. C., Ng, L. T., Lin, L. T., Richardson, C. D., Wang, G. H., & Lin, C. C. (2010). Resveratrol arrests cell cycle and induces apoptosis in human hepatocellular carcinoma Huh-7 cells. Journal of Medicinal Food, 13(6), 1415-1423.

Resveratrol arrests cell cycle and induces apoptosis in human hepatocellular carcinoma Huh-7 cells. / Liao, Pei Chi; Ng, Lean Teik; Lin, Liang Tzung; Richardson, Christopher D.; Wang, Guey Horng; Lin, Chun Ching.

In: Journal of Medicinal Food, Vol. 13, No. 6, 12.2010, p. 1415-1423.

Research output: Contribution to journalArticle

Liao, PC, Ng, LT, Lin, LT, Richardson, CD, Wang, GH & Lin, CC 2010, 'Resveratrol arrests cell cycle and induces apoptosis in human hepatocellular carcinoma Huh-7 cells.', Journal of Medicinal Food, vol. 13, no. 6, pp. 1415-1423.
Liao, Pei Chi ; Ng, Lean Teik ; Lin, Liang Tzung ; Richardson, Christopher D. ; Wang, Guey Horng ; Lin, Chun Ching. / Resveratrol arrests cell cycle and induces apoptosis in human hepatocellular carcinoma Huh-7 cells. In: Journal of Medicinal Food. 2010 ; Vol. 13, No. 6. pp. 1415-1423.
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