Resveratrol arrests cell cycle and induces apoptosis in human hepatocellular carcinoma Huh-7 cells.

Pei Chi Liao, Lean Teik Ng, Liang Tzung Lin, Christopher D. Richardson, Guey Horng Wang, Chun Ching Lin

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Abstract

Resveratrol has been shown to possess anticancer, anti-aging, anti-inflammatory, antimicrobial, and neuroprotective activities. In this study, we examined the antiproliferative properties of resveratrol and its molecular mechanism(s) of action in Huh-7 cells, a new human hepatoma cell line system for hepatitis C virus. Results showed that resveratrol significantly inhibited Huh-7 cell proliferation (50% inhibitory concentration = 22.4 μg/mL) and effectively induced cell cycle arrest and apoptosis. It up-regulated p21/WAF1 expression in a p53-independent manner, but the expressions of cyclin E, cyclin A, and cyclin-dependent kinase 2 were down-regulated. It also caused an increase in the ratio of pro-apoptotic/anti-apoptotic protein, which was associated with the mitochondrial membrane depolarization and the increase in caspase activity. Resveratrol showed no effect on Fas, Fas ligand, extracellular signal regulated kinase (ERK) 1/2, and p38 expression but down-regulated phospho-ERK and phospho-p38 expression. In addition, resveratrol was noted to trigger autophagic cell death through the increased expression of autophagy-related Atg5, Atg7, Atg9, and Atg12 proteins. These results suggest that resveratrol could be an important chemoprevention agent for hepatoma of hepatitis C virus infection.

Original languageEnglish
Pages (from-to)1415-1423
Number of pages9
JournalJournal of Medicinal Food
Volume13
Issue number6
Publication statusPublished - Dec 2010
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Liao, P. C., Ng, L. T., Lin, L. T., Richardson, C. D., Wang, G. H., & Lin, C. C. (2010). Resveratrol arrests cell cycle and induces apoptosis in human hepatocellular carcinoma Huh-7 cells. Journal of Medicinal Food, 13(6), 1415-1423.