Resveratrol, a red wine antioxidant, reduces atrial fibrillation susceptibility in the failing heart by PI3K/AKT/eNOS signaling pathway activation

Eric Chong, Shih Lin Chang, Ya Wen Hsiao, Rahul Singhal, Shuen Hsin Liu, Trung Leha, Wen Yu Lin, Chiao Po Hsu, Yao Chang Chen, Yi Jen Chen, Tsu Juey Wu, Satoshi Higa, Shih Ann Chen

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background Resveratrol has shown benefits in reducing ventricular remodeling and arrhythmias. Objective This study aimed to assess the therapeutic efficacy of resveratrol in reducing atrial fibrillation (AF) in a heart failure (HF) model and to explore the underlying mechanisms. Methods HF rabbits were created 4 weeks after undergoing coronary ligation. Group 1 (n = 6) was divided into subgroups of (a) normal rabbits, (b) HF sham rabbits, and (c) HF rabbits treated for 1 week with intraperitoneal injections of resveratrol, (d) resveratrol plus wortmannin, or (e) resveratrol plus diphenyleneiodonium chloride (DPI). All rabbits underwent epicardial catheter stimulation. Collagen content, messenger RNA and protein expression in ion channels, and phosphoinositide 3-kinase (PI3K)/AKT/endothelial nitric oxide synthase (eNOS) signaling pathways were studied in left atrial appendage (LAA) preparations. To investigate acute drug effects on left atrial electrophysiology, groups 2 a through 2e (n = 6 per group) were subjected to Langendorff perfusion. Results Higher AF inducibility was found in the HF group and groups that were given PI3K and eNOS inhibitors than in the normal and resveratrol-treated groups (P

Original languageEnglish
Article number6115
Pages (from-to)1046-1056
Number of pages11
JournalHeart Rhythm
Volume12
Issue number5
DOIs
Publication statusPublished - May 1 2015

Fingerprint

1-Phosphatidylinositol 4-Kinase
Nitric Oxide Synthase Type III
Wine
Atrial Fibrillation
Antioxidants
Heart Failure
Rabbits
Atrial Appendage
Ventricular Remodeling
Electrophysiology
Intraperitoneal Injections
Ion Channels
Ligation
resveratrol
Cardiac Arrhythmias
Collagen
Catheters
Perfusion
Messenger RNA
Pharmaceutical Preparations

Keywords

  • Atrial fibrillation
  • Ion channel
  • Resveratrol
  • Signaling pathway

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Resveratrol, a red wine antioxidant, reduces atrial fibrillation susceptibility in the failing heart by PI3K/AKT/eNOS signaling pathway activation. / Chong, Eric; Chang, Shih Lin; Hsiao, Ya Wen; Singhal, Rahul; Liu, Shuen Hsin; Leha, Trung; Lin, Wen Yu; Hsu, Chiao Po; Chen, Yao Chang; Chen, Yi Jen; Wu, Tsu Juey; Higa, Satoshi; Chen, Shih Ann.

In: Heart Rhythm, Vol. 12, No. 5, 6115, 01.05.2015, p. 1046-1056.

Research output: Contribution to journalArticle

Chong, E, Chang, SL, Hsiao, YW, Singhal, R, Liu, SH, Leha, T, Lin, WY, Hsu, CP, Chen, YC, Chen, YJ, Wu, TJ, Higa, S & Chen, SA 2015, 'Resveratrol, a red wine antioxidant, reduces atrial fibrillation susceptibility in the failing heart by PI3K/AKT/eNOS signaling pathway activation', Heart Rhythm, vol. 12, no. 5, 6115, pp. 1046-1056. https://doi.org/10.1016/j.hrthm.2015.01.044
Chong, Eric ; Chang, Shih Lin ; Hsiao, Ya Wen ; Singhal, Rahul ; Liu, Shuen Hsin ; Leha, Trung ; Lin, Wen Yu ; Hsu, Chiao Po ; Chen, Yao Chang ; Chen, Yi Jen ; Wu, Tsu Juey ; Higa, Satoshi ; Chen, Shih Ann. / Resveratrol, a red wine antioxidant, reduces atrial fibrillation susceptibility in the failing heart by PI3K/AKT/eNOS signaling pathway activation. In: Heart Rhythm. 2015 ; Vol. 12, No. 5. pp. 1046-1056.
@article{a27176c5c03243b283968b7d43b62783,
title = "Resveratrol, a red wine antioxidant, reduces atrial fibrillation susceptibility in the failing heart by PI3K/AKT/eNOS signaling pathway activation",
abstract = "Background Resveratrol has shown benefits in reducing ventricular remodeling and arrhythmias. Objective This study aimed to assess the therapeutic efficacy of resveratrol in reducing atrial fibrillation (AF) in a heart failure (HF) model and to explore the underlying mechanisms. Methods HF rabbits were created 4 weeks after undergoing coronary ligation. Group 1 (n = 6) was divided into subgroups of (a) normal rabbits, (b) HF sham rabbits, and (c) HF rabbits treated for 1 week with intraperitoneal injections of resveratrol, (d) resveratrol plus wortmannin, or (e) resveratrol plus diphenyleneiodonium chloride (DPI). All rabbits underwent epicardial catheter stimulation. Collagen content, messenger RNA and protein expression in ion channels, and phosphoinositide 3-kinase (PI3K)/AKT/endothelial nitric oxide synthase (eNOS) signaling pathways were studied in left atrial appendage (LAA) preparations. To investigate acute drug effects on left atrial electrophysiology, groups 2 a through 2e (n = 6 per group) were subjected to Langendorff perfusion. Results Higher AF inducibility was found in the HF group and groups that were given PI3K and eNOS inhibitors than in the normal and resveratrol-treated groups (P",
keywords = "Atrial fibrillation, Ion channel, Resveratrol, Signaling pathway",
author = "Eric Chong and Chang, {Shih Lin} and Hsiao, {Ya Wen} and Rahul Singhal and Liu, {Shuen Hsin} and Trung Leha and Lin, {Wen Yu} and Hsu, {Chiao Po} and Chen, {Yao Chang} and Chen, {Yi Jen} and Wu, {Tsu Juey} and Satoshi Higa and Chen, {Shih Ann}",
year = "2015",
month = "5",
day = "1",
doi = "10.1016/j.hrthm.2015.01.044",
language = "English",
volume = "12",
pages = "1046--1056",
journal = "Heart Rhythm",
issn = "1547-5271",
publisher = "Elsevier",
number = "5",

}

TY - JOUR

T1 - Resveratrol, a red wine antioxidant, reduces atrial fibrillation susceptibility in the failing heart by PI3K/AKT/eNOS signaling pathway activation

AU - Chong, Eric

AU - Chang, Shih Lin

AU - Hsiao, Ya Wen

AU - Singhal, Rahul

AU - Liu, Shuen Hsin

AU - Leha, Trung

AU - Lin, Wen Yu

AU - Hsu, Chiao Po

AU - Chen, Yao Chang

AU - Chen, Yi Jen

AU - Wu, Tsu Juey

AU - Higa, Satoshi

AU - Chen, Shih Ann

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Background Resveratrol has shown benefits in reducing ventricular remodeling and arrhythmias. Objective This study aimed to assess the therapeutic efficacy of resveratrol in reducing atrial fibrillation (AF) in a heart failure (HF) model and to explore the underlying mechanisms. Methods HF rabbits were created 4 weeks after undergoing coronary ligation. Group 1 (n = 6) was divided into subgroups of (a) normal rabbits, (b) HF sham rabbits, and (c) HF rabbits treated for 1 week with intraperitoneal injections of resveratrol, (d) resveratrol plus wortmannin, or (e) resveratrol plus diphenyleneiodonium chloride (DPI). All rabbits underwent epicardial catheter stimulation. Collagen content, messenger RNA and protein expression in ion channels, and phosphoinositide 3-kinase (PI3K)/AKT/endothelial nitric oxide synthase (eNOS) signaling pathways were studied in left atrial appendage (LAA) preparations. To investigate acute drug effects on left atrial electrophysiology, groups 2 a through 2e (n = 6 per group) were subjected to Langendorff perfusion. Results Higher AF inducibility was found in the HF group and groups that were given PI3K and eNOS inhibitors than in the normal and resveratrol-treated groups (P

AB - Background Resveratrol has shown benefits in reducing ventricular remodeling and arrhythmias. Objective This study aimed to assess the therapeutic efficacy of resveratrol in reducing atrial fibrillation (AF) in a heart failure (HF) model and to explore the underlying mechanisms. Methods HF rabbits were created 4 weeks after undergoing coronary ligation. Group 1 (n = 6) was divided into subgroups of (a) normal rabbits, (b) HF sham rabbits, and (c) HF rabbits treated for 1 week with intraperitoneal injections of resveratrol, (d) resveratrol plus wortmannin, or (e) resveratrol plus diphenyleneiodonium chloride (DPI). All rabbits underwent epicardial catheter stimulation. Collagen content, messenger RNA and protein expression in ion channels, and phosphoinositide 3-kinase (PI3K)/AKT/endothelial nitric oxide synthase (eNOS) signaling pathways were studied in left atrial appendage (LAA) preparations. To investigate acute drug effects on left atrial electrophysiology, groups 2 a through 2e (n = 6 per group) were subjected to Langendorff perfusion. Results Higher AF inducibility was found in the HF group and groups that were given PI3K and eNOS inhibitors than in the normal and resveratrol-treated groups (P

KW - Atrial fibrillation

KW - Ion channel

KW - Resveratrol

KW - Signaling pathway

UR - http://www.scopus.com/inward/record.url?scp=84928479175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928479175&partnerID=8YFLogxK

U2 - 10.1016/j.hrthm.2015.01.044

DO - 10.1016/j.hrthm.2015.01.044

M3 - Article

VL - 12

SP - 1046

EP - 1056

JO - Heart Rhythm

JF - Heart Rhythm

SN - 1547-5271

IS - 5

M1 - 6115

ER -