Restriction fragment length polymorphism analysis to study the genetic origin of complete hydatidiform mole

Tsang Ming Ko, Chang Yao Hsieh, Hong Nerng Ho, Fon Jou Hsieh, Tzu Yao Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To determine the genetic origin of the complete hydatidiform mole, 20 abnormal pregnancies were studied with restriction fragment length polymorphism with five genomic probes: EJ 6.6, β-globin gene, 3′α-hypervariable region, J-Bir, and St14. In the 12 cases of molar pregnancy, pure paternal origin was proved in 11 cases, but both maternal and paternal inheritance were shown in only one case. In the cases with pure paternal origin, all of the restriction fragment length polymorphisms were homozygous, although those of the fathers were heterozygous at 15 loci. In the four cases that mimicked hydatidiform mole but were diagnosed as hydropic change of villi, both paternal and maternal inheritance were noted. In the four pregnancies with blighted ovum, both paternal and maternal inheritance were shown in three cases; and in one case with a balanced translocation between chromosomes 13 and 14, only paternal inheritance was noted. This study showed that most of the complete hydatidiform moles were caused by fertilization of an empty egg by a duplicated haploid sperm, but rare exceptions may exist.

Original languageEnglish
Pages (from-to)901-906
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume164
Issue number3
DOIs
Publication statusPublished - Jan 1 1991
Externally publishedYes

Fingerprint

Hydatidiform Mole
Restriction Fragment Length Polymorphisms
Ovum
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 13
Pregnancy
beta-Globins
Haploidy
Fertilization
Fathers
Spermatozoa
Edema
Paternal Inheritance
Genes
Maternal Inheritance

Keywords

  • Complete hydatidiform mole
  • genetic origin
  • restriction fragment length polymorphism

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Restriction fragment length polymorphism analysis to study the genetic origin of complete hydatidiform mole. / Ko, Tsang Ming; Hsieh, Chang Yao; Ho, Hong Nerng; Hsieh, Fon Jou; Lee, Tzu Yao.

In: American Journal of Obstetrics and Gynecology, Vol. 164, No. 3, 01.01.1991, p. 901-906.

Research output: Contribution to journalArticle

Ko, Tsang Ming ; Hsieh, Chang Yao ; Ho, Hong Nerng ; Hsieh, Fon Jou ; Lee, Tzu Yao. / Restriction fragment length polymorphism analysis to study the genetic origin of complete hydatidiform mole. In: American Journal of Obstetrics and Gynecology. 1991 ; Vol. 164, No. 3. pp. 901-906.
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