The purpose of this study was to investigate the effects of various thicknesses of titanium oxide and pore size/roughness (micrometer range) on the initial attachment and proliferation of osteoblast-like cells (MG-63) to the implant surfaces in vitro. The experiment was designed to examine 2 categories: (A) thicknesses of the titanium oxide and (B) pore sizes/roughness. The results showed that the anodizing oxide layer contained TiO2 in different phases. Chemical composition and contact angles were various as the thickness of oxidation layer increased. The optical density from the MTT test showed that cells significantly proliferated on titanium samples except with 50-and 100-μm pore size after 48 hours. Titanium oxide with thicknesses of 40 and 80nm showed higher levels of cell proliferation with statistical significant differences on the 8th and 12th days. Titanium discs with 100-μm pore size showed the greatest optical density of initial cell attachment with a statistically significant difference compared with that of 10-μm pore size. Smaller pore sizes and smooth surfaces presented greater cell proliferation than larger ones but no statistical significant difference was shown on the 8th and 12th days. Based on the above investigation, surface roughness with increased surface areas was considered, when the relationship between contact angle and cell attachment level was investigated in this work.
|Original language||Traditional Chinese|
|Number of pages||10|
|Publication status||Published - 2004|
- titanium oxide thickness
- pro size
- cell attachment