電化學方式形成不同氧化膜層厚度及孔徑大小於鈦金屬表面對類造骨細胞之影響

Translated title of the contribution: Response of Osteoblast-like Cells to Electro-chemically Treated Titanium Surfaces of Various Oxide Thicknesses and Pore Sizes

楊政峰, 歐耿良, 周幸華, 林哲堂, 施永勳, 薛文景, 鄧乃嘉, 曲國田, 李勝揚

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to investigate the effects of various thicknesses of titanium oxide and pore size/roughness (micrometer range) on the initial attachment and proliferation of osteoblast-like cells (MG-63) to the implant surfaces in vitro. The experiment was designed to examine 2 categories: (A) thicknesses of the titanium oxide and (B) pore sizes/roughness. The results showed that the anodizing oxide layer contained TiO2 in different phases. Chemical composition and contact angles were various as the thickness of oxidation layer increased. The optical density from the MTT test showed that cells significantly proliferated on titanium samples except with 50-and 100-μm pore size after 48 hours. Titanium oxide with thicknesses of 40 and 80nm showed higher levels of cell proliferation with statistical significant differences on the 8th and 12th days. Titanium discs with 100-μm pore size showed the greatest optical density of initial cell attachment with a statistically significant difference compared with that of 10-μm pore size. Smaller pore sizes and smooth surfaces presented greater cell proliferation than larger ones but no statistical significant difference was shown on the 8th and 12th days. Based on the above investigation, surface roughness with increased surface areas was considered, when the relationship between contact angle and cell attachment level was investigated in this work.
Original languageTraditional Chinese
Pages (from-to)139-148
Number of pages10
Journal中華牙醫學雜誌(中文版)
Volume23
Issue number2
Publication statusPublished - 2004

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Keywords

  • anodization
  • titanium oxide thickness
  • pro size
  • cell attachment
  • proliferation

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