Resistin mRNA levels are downregulated by estrogen in vivo and in vitro

Seng Wong Huang, Kok Min Seow, Low Tone Ho, Yueh Chien, Dong Yue Chung, Chih Ling Chang, Ying Hsiu Lai, Jiann Loung Hwang, Chi Chang Juan

Research output: Contribution to journalArticle

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Abstract

Resistin, a hormone secreted by adipocytes, is suggested to be an important link between obesity and diabetes. The aim of this study was to evaluate the regulatory effect of estrogen on adipocyte resistin gene expression in ovariectomized (OVX) rats and in isolated rat adipocytes in vitro. Subcutaneous injection of estradiol benzoate reduced resistin mRNA levels in adipocytes isolated from the inguinal, parametrial, perirenal, retroperitoneal, or periovarian fat deposits of OVX rats, while an in vitro study showed that estradiol treatment decreased resistin mRNA levels in cultured rat periovarian fat adipocytes. Results of Western blotting analysis also showed that estrogen decreased adipose resistin contents in vivo and in vitro. These data suggest that estrogen is a pivotal negative regulator of resistin gene expression.

Original languageEnglish
Pages (from-to)449-454
Number of pages6
JournalFEBS Letters
Volume579
Issue number2
DOIs
Publication statusPublished - Jan 17 2005

Fingerprint

Resistin
Estrogens
Adipocytes
Down-Regulation
Messenger RNA
Rats
Gene expression
Fats
Gene Expression
Groin
Subcutaneous Injections
Regulator Genes
Medical problems
In Vitro Techniques
Estradiol
Deposits
Obesity
Western Blotting
Hormones

Keywords

  • Adipocyte
  • Estrogen
  • Ovariectomy
  • Resistin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Huang, S. W., Seow, K. M., Ho, L. T., Chien, Y., Chung, D. Y., Chang, C. L., ... Juan, C. C. (2005). Resistin mRNA levels are downregulated by estrogen in vivo and in vitro. FEBS Letters, 579(2), 449-454. https://doi.org/10.1016/j.febslet.2004.12.010

Resistin mRNA levels are downregulated by estrogen in vivo and in vitro. / Huang, Seng Wong; Seow, Kok Min; Ho, Low Tone; Chien, Yueh; Chung, Dong Yue; Chang, Chih Ling; Lai, Ying Hsiu; Hwang, Jiann Loung; Juan, Chi Chang.

In: FEBS Letters, Vol. 579, No. 2, 17.01.2005, p. 449-454.

Research output: Contribution to journalArticle

Huang, SW, Seow, KM, Ho, LT, Chien, Y, Chung, DY, Chang, CL, Lai, YH, Hwang, JL & Juan, CC 2005, 'Resistin mRNA levels are downregulated by estrogen in vivo and in vitro', FEBS Letters, vol. 579, no. 2, pp. 449-454. https://doi.org/10.1016/j.febslet.2004.12.010
Huang SW, Seow KM, Ho LT, Chien Y, Chung DY, Chang CL et al. Resistin mRNA levels are downregulated by estrogen in vivo and in vitro. FEBS Letters. 2005 Jan 17;579(2):449-454. https://doi.org/10.1016/j.febslet.2004.12.010
Huang, Seng Wong ; Seow, Kok Min ; Ho, Low Tone ; Chien, Yueh ; Chung, Dong Yue ; Chang, Chih Ling ; Lai, Ying Hsiu ; Hwang, Jiann Loung ; Juan, Chi Chang. / Resistin mRNA levels are downregulated by estrogen in vivo and in vitro. In: FEBS Letters. 2005 ; Vol. 579, No. 2. pp. 449-454.
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