Abstract

The effects of residual intra-substrate stress distribution on cell behavior have not been systematically investigated. Thus, the objective of this research was to analyze the relationship between cell distribution and internal stress patterns. A photoelastic method was used for residual stresses identification. Poly-l-lactide (PLLA) discs were prepared using an injection molding technique. MG-63 and NIH-3T3 cells were cultured on the surface of the PLLA disc. The cell distributions for high and low-stress regions were measured and compared. There were significantly more cells in the low-stress regions relative to high-stress analogs (p <0.05). Further, linear relationships were demonstrated for both MG-63 and NIH-3T3 models with high correlation coefficients of 0.80 and 0.95, respectively. These results suggest that the distribution of residual stress in substrates affect cell behavior. These findings may provide greater insight into the interaction between cells and substrates, and may serve as a useful reference in future clinical study.

Original languageEnglish
Pages (from-to)513-521
Number of pages9
JournalAnnals of Biomedical Engineering
Volume36
Issue number3
DOIs
Publication statusPublished - Mar 2008

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Residual stresses
Substrates
Injection molding
Stress concentration

Keywords

  • Mechanical sensing
  • Osteoblast
  • Poly-L-lactide
  • Residual stress

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Residual stress patterns affect cell distributions on injection-molded poly-l-lactide substrate. / Lee, Sheng Yang; Tseng, How; Ou, Keng Liang; Yang, Jen Chang; Ho, Kuo Ning; Lin, Che Tong; Huang, Haw Ming.

In: Annals of Biomedical Engineering, Vol. 36, No. 3, 03.2008, p. 513-521.

Research output: Contribution to journalArticle

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abstract = "The effects of residual intra-substrate stress distribution on cell behavior have not been systematically investigated. Thus, the objective of this research was to analyze the relationship between cell distribution and internal stress patterns. A photoelastic method was used for residual stresses identification. Poly-l-lactide (PLLA) discs were prepared using an injection molding technique. MG-63 and NIH-3T3 cells were cultured on the surface of the PLLA disc. The cell distributions for high and low-stress regions were measured and compared. There were significantly more cells in the low-stress regions relative to high-stress analogs (p <0.05). Further, linear relationships were demonstrated for both MG-63 and NIH-3T3 models with high correlation coefficients of 0.80 and 0.95, respectively. These results suggest that the distribution of residual stress in substrates affect cell behavior. These findings may provide greater insight into the interaction between cells and substrates, and may serve as a useful reference in future clinical study.",
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AU - Lee, Sheng Yang

AU - Tseng, How

AU - Ou, Keng Liang

AU - Yang, Jen Chang

AU - Ho, Kuo Ning

AU - Lin, Che Tong

AU - Huang, Haw Ming

PY - 2008/3

Y1 - 2008/3

N2 - The effects of residual intra-substrate stress distribution on cell behavior have not been systematically investigated. Thus, the objective of this research was to analyze the relationship between cell distribution and internal stress patterns. A photoelastic method was used for residual stresses identification. Poly-l-lactide (PLLA) discs were prepared using an injection molding technique. MG-63 and NIH-3T3 cells were cultured on the surface of the PLLA disc. The cell distributions for high and low-stress regions were measured and compared. There were significantly more cells in the low-stress regions relative to high-stress analogs (p <0.05). Further, linear relationships were demonstrated for both MG-63 and NIH-3T3 models with high correlation coefficients of 0.80 and 0.95, respectively. These results suggest that the distribution of residual stress in substrates affect cell behavior. These findings may provide greater insight into the interaction between cells and substrates, and may serve as a useful reference in future clinical study.

AB - The effects of residual intra-substrate stress distribution on cell behavior have not been systematically investigated. Thus, the objective of this research was to analyze the relationship between cell distribution and internal stress patterns. A photoelastic method was used for residual stresses identification. Poly-l-lactide (PLLA) discs were prepared using an injection molding technique. MG-63 and NIH-3T3 cells were cultured on the surface of the PLLA disc. The cell distributions for high and low-stress regions were measured and compared. There were significantly more cells in the low-stress regions relative to high-stress analogs (p <0.05). Further, linear relationships were demonstrated for both MG-63 and NIH-3T3 models with high correlation coefficients of 0.80 and 0.95, respectively. These results suggest that the distribution of residual stress in substrates affect cell behavior. These findings may provide greater insight into the interaction between cells and substrates, and may serve as a useful reference in future clinical study.

KW - Mechanical sensing

KW - Osteoblast

KW - Poly-L-lactide

KW - Residual stress

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