Replication of chromosome 4 candidate region for alcohol addiction through DNA pooling and individual genotype association studies with the marker D4S2407 in alcoholic and control japanese groups

H. Koch, L. Hill, E. W. Loh, S. Higuchi, D. Ball, I. W. Craig

Research output: Contribution to journalArticle

Abstract

A number of chromosomal locations potentially implicated in the etiology of alcohol addiction have been identified by linkage analysis (e.g. see Reich et al, 1998, Neuropsychiatrie Genetics 81: 207-215). We have investigated potential allelic association for markers within and flanking the cluster of alcohol dehydrogenase genes on chromosome 4 with alcoholism in the Japanese population. Allele image patterns (AIPs) for pooled samples (65 individuals from each group) have been generated and examined for significant differences - employing tech niques described previously (Hill et al, 1999, NeuroReport 10: 843-848) and any potentially significant results examined further by individual genotyping. Of the loci examined, allele patterns at both the D4S2361 and D4S2407 loci gave a delta AIP value of >0.25. On individual genotyping, no significant allele frequency differences between case and controls were observed for D4S2361 (p value for "clump" analysis = 0.8). In marked contrast, individual genotyping for D4S2407 indicated that the allele frequencies for the two groups were significantly different ("clump" analysis p = 0.01). Furthermore, a 2 x2 2 test for the nominated allele gave a p value = 0.002. These data provide strong motivation for a detailed investigation of the individual ADH genes which lie close to this marker.

Original languageEnglish
Number of pages1
JournalAmerican Journal of Medical Genetics - Neuropsychiatric Genetics
Volume96
Issue number4
Publication statusPublished - Aug 7 2000
Externally publishedYes

ASJC Scopus subject areas

  • Genetics(clinical)
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience

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