Abstract

A recent study demonstrated that an increased risk of chronic kidney disease (CKD) was associated with high urinary total arsenic levels. However, whether genomic instability is related to CKD remains unclear. An association between CKD and genetic polymorphisms of regulation enzymes of the renin-angiotensin-aldosterone system (RAAS), such as angiotensin-converting enzyme ( ACE), angiotensinogen ( AGT), angiotensin II type I receptor ( AT1R), and aldosterone synthase ( CYP11B2) has not been shown. The aim of the present study was to investigate the relationship between arsenic, genetic polymorphisms of RAAS enzymes and CKD. A total of 233 patients and 449 age- and gender-matched controls were recruited from the Taipei Medical University Hospital, Taipei Municipal Wan Fang Hospital and the Shin Kong Wu Ho-Su Memorial Hospital. Concentrations of urinary arsenic were determined by a high-performance liquid chromatography-linked hydride generator, and atomic absorption spectrometry. Polymorphisms of ACE(I/D), AGT(A[-. 20]C), (T174M), (M235T), AT1R(A1166C) and CYP11B2(C[-. 344]T) were examined by polymerase chain reaction and restriction fragment length polymorphism. Subjects carrying the CYP11B2 TT genotype had a higher odds ratio (OR), 1.39 (0.96-2.01), of CKD; while those with the AGT(A[-. 20]C) CC genotype had an inverse OR of CKD (0.20 (0.05-0.81)), and a high-risk genotype was defined as A/A. +. A/C for AGT(A[-. 20C]) and T/T for CYP11B2(C[-. 344]T). The trend test showed a higher OR for CKD in patients who had either high urinary total arsenic levels or carried the high-risk genotype, or both, compared to patients with low urinary total arsenic levels, who carried the low-risk genotype, and could also be affected by the hypertension or diabetes status.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalToxicology and Applied Pharmacology
Volume279
Issue number2
DOIs
Publication statusPublished - Sep 1 2014

Fingerprint

Angiotensins
Arsenic
Cytochrome P-450 CYP11B2
Renin-Angiotensin System
Aldosterone
Polymorphism
Chronic Renal Insufficiency
Renin
Genes
Angiotensinogen
Genotype
Odds Ratio
Peptidyl-Dipeptidase A
Genetic Polymorphisms
Angiotensin I
Atomic absorption spectrometry
Angiotensin Receptors
Genomic Instability
Polymerase chain reaction
High performance liquid chromatography

Keywords

  • Aldosterone synthase (CYP11B2)
  • Angiotensinogen (AGT)
  • Arsenic
  • Chronic kidney disease
  • Gene polymorphisms
  • Renin-angiotensin-aldosterone system

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Renin-angiotensin-aldosterone system related gene polymorphisms and urinary total arsenic is related to chronic kidney disease. / Chen, Wei Jen; Huang, Ya Li; Shiue, Horng Sheng; Chen, Tzen-Wen; Lin, Yuh Feng; Huang, Chao Yuan; Lin, Ying-Chin; Han, Bor Cheng; Hsueh, Yu Mei.

In: Toxicology and Applied Pharmacology, Vol. 279, No. 2, 01.09.2014, p. 95-102.

Research output: Contribution to journalArticle

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