Renal protective effect of Xiao-Chai-Hu-Tang on diabetic nephropathy of type 1-diabetic mice

Chun Ching Lin, Liang-Tzung Lin, Ming Hong Yen, Juei Tang Cheng, Chung-Hsi Hsing, Ching Hua Yeh

Research output: Contribution to journalArticle

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Abstract

Xiao-Chai-Hu-Tang (XCHT), a traditional Chinese medicine formula consisting of seven medicinal plants, is used in the treatment of various diseases. We show here that XCHT could protect type-1 diabetic mice against diabetic nephropathy, using streptozotocin (STZ)-induced diabetic mice and high-glucose (HG)-exposed rat mesangial cell (RMC) as models. Following 4 weeks of oral administration with XCHT, renal functions and renal hypertrophy significantly improved in the STZ-diabetic mice, while serum glucose was only moderately reduced compared to vehicle treatment. Treatment with XCHT in the STZ-diabetic mice and HG-exposed RMC resulted in a decrease in expression levels of TGF-1, fibronectin, and collagen IV, with concomitant increase in BMP-7 expression. Data from DPPH assay, DHE stain, and CM-H2DCFDA analysis indicated that XCHT could scavenge free radicals and inhibit high-glucose-induced ROS in RMCs. Taken together, these results suggest that treatment with XCHT can improve renal functions in STZ-diabetic mice, an effect that is potentially mediated through decreasing oxidative stress and production of TGF-β1, fibronectin, and collagen IV in the kidney during development of diabetic nephropathy. XCHT, therefore merits further investigation for application to improve renal functions in diabetic disorders.

Original languageEnglish
Article number984024
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012

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Diabetic Nephropathies
Kidney
Streptozocin
Glucose
Mesangial Cells
Fibronectins
Collagen
Bone Morphogenetic Protein 7
Chinese Traditional Medicine
Therapeutics
Medicinal Plants
shosaiko-to
Hypertrophy
Free Radicals
Oral Administration
Oxidative Stress
Coloring Agents
Serum

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Renal protective effect of Xiao-Chai-Hu-Tang on diabetic nephropathy of type 1-diabetic mice. / Lin, Chun Ching; Lin, Liang-Tzung; Yen, Ming Hong; Cheng, Juei Tang; Hsing, Chung-Hsi; Yeh, Ching Hua.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 984024, 2012.

Research output: Contribution to journalArticle

Lin, Chun Ching ; Lin, Liang-Tzung ; Yen, Ming Hong ; Cheng, Juei Tang ; Hsing, Chung-Hsi ; Yeh, Ching Hua. / Renal protective effect of Xiao-Chai-Hu-Tang on diabetic nephropathy of type 1-diabetic mice. In: Evidence-based Complementary and Alternative Medicine. 2012 ; Vol. 2012.
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