Renal ischemia/reperfusion injury inhibits differentiation of dendritic cells derived from bone marrow monocytes in rats

Chih J. Wu, Joen Rong Sheu, Han Hsiang Chen, Hui F. Liao, Yuh Cheng Yang, Stone Yang, Yu J. Chen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Dendritic cells (DCs) are impacted by surgical injury, exercise, and other physiological stressors. This study aims to determine whether renal I/R injury affects 1) the differentiation of myeloid DCs from bone marrow monocytes (BMMos) and the maturation and activation state of these DCs and 2) DC infiltration of kidney. Sprague-Dawley rats were subjected to I/R injury or sham-operated. Creatinine clearance was monitored daily during the 14 d of reperfusion that followed the ischemic insult. At 2 and 14 d of reperfusion, the following were assessed 1) properties of BMMo-derived DCs (i.e., the amount of generated DCs, differentiation state markers [CD11c, CD80, CD86, and Ia], and functional state [MLR and amount of IL-12 produced]), and 2) the presence of DCs in the kidney. Numbers of BMMo-derived DCs were significantly decreased in the I/R injured group (compared with the sham-operated group) at 2 d but not 14 d. A comparison of the their functionality found mixed lymphocyte response [MLR] and IL-12 production were similar in the two groups at both time points. Also, immunohistochemistry showed infiltrating DCs in the outer medulla of the I/R injured kidney at 2 d but not 14 d of reperfusion. Thus, I/R stress reduces the number of DCs differentiated from BMMos but not the functional activity of these DCs. This decrease may reflect a stress-induced downshift in the capacity of BMMos to differentiate into DCs and a parallel upshift in the capacity of DCs to infiltrate the kidney.

Original languageEnglish
Pages (from-to)1121-1128
Number of pages8
JournalLife Sciences
Volume78
Issue number10
DOIs
Publication statusPublished - Feb 2 2006

Fingerprint

Reperfusion Injury
Dendritic Cells
Rats
Monocytes
Bone
Bone Marrow
Kidney
Reperfusion
Lymphocytes
Interleukin-12
Differentiation Antigens
Intraoperative Complications
Wounds and Injuries
Myeloid Cells
Infiltration
Interleukin-2
Sprague Dawley Rats
Cell Differentiation
Creatinine
Chemical activation

Keywords

  • Bone marrow-derived dendritic cells
  • Differentiation
  • Ischemia-reperfusion injury
  • Myeloid dendritic cells

ASJC Scopus subject areas

  • Pharmacology

Cite this

Renal ischemia/reperfusion injury inhibits differentiation of dendritic cells derived from bone marrow monocytes in rats. / Wu, Chih J.; Sheu, Joen Rong; Chen, Han Hsiang; Liao, Hui F.; Yang, Yuh Cheng; Yang, Stone; Chen, Yu J.

In: Life Sciences, Vol. 78, No. 10, 02.02.2006, p. 1121-1128.

Research output: Contribution to journalArticle

Wu, Chih J. ; Sheu, Joen Rong ; Chen, Han Hsiang ; Liao, Hui F. ; Yang, Yuh Cheng ; Yang, Stone ; Chen, Yu J. / Renal ischemia/reperfusion injury inhibits differentiation of dendritic cells derived from bone marrow monocytes in rats. In: Life Sciences. 2006 ; Vol. 78, No. 10. pp. 1121-1128.
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