Remote effects on the striatal dopamine system after fluid percussion injury

Eagle Yi Kung Huang, Tung Han Tsai, Tung Tai Kuo, Jing Jr Tsai, Pi Fen Tsui, Yu Ching Chou, Hsin I. Ma, Yung Hsiao Chiang, Yuan Hao Chen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Purpose: To investigate the effects of traumatic brain injury (TBI) on the dopamine system in the brain at different distances from the impaction site, we compared the release, reuptake, metabolism, and release probability of dopamine on the sides of the brain ipsilateral and contralateral to the injury at different time points after varying severities of fluid percussion injuries. Materials and methods: Tonic (1-pulse evoked) and bursting (10-pulse evoked) dopamine release changes in the ipsilateral and contralateral sides of the striatum resulting from mild (2-Pa) and severe (6-Pa) levels of fluid percussion injury were analyzed at the acute (2. h and 24. h), subacute (1 and 2 weeks), and chronic stages (4, 6, and 8 weeks) after injury by using fast scan cyclic voltammetry to measure brain slices. The metabolic rate of striatal dopamine was surveyed using high-performance liquid chromatography. The microglia reaction was analyzed using immunohistochemistry at each stage. Results: In 6-Pa injured animals, for both tonic and bursting dopamine release, reuptake and release probability were suppressed on both the ipsilateral and contralateral sides of the striatum from the acute to the chronic stage. These neuronal activities were also affected at the subacute stage on both sides of the striatum in 2-Pa injured animals. The turnover rate of dopamine was not affected in the 2-Pa injured animals but increased gradually during the chronic stage in the 6-Pa injured group. Conclusion: TBI suppresses dopamine release and reuptake and affects the metabolic rate and release probability of dopamine on the sides of the nigrostriatal system both ipsilateral and contralateral to the injury during both the acute and subacute stages after the injury.

Original languageEnglish
Pages (from-to)156-172
Number of pages17
JournalBehavioural Brain Research
Volume267
DOIs
Publication statusPublished - Jul 1 2014

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Percussion
Corpus Striatum
Dopamine
Wounds and Injuries
Brain
Microglia
Immunohistochemistry
High Pressure Liquid Chromatography

Keywords

  • Dopamine releasing and reuptake
  • Fast-Scan Cyclic Voltammetry (FSCV)
  • Fluid percussion injury
  • High-pressure liquid chromatography (HPLC)
  • Neuroinflammation

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Medicine(all)

Cite this

Huang, E. Y. K., Tsai, T. H., Kuo, T. T., Tsai, J. J., Tsui, P. F., Chou, Y. C., ... Chen, Y. H. (2014). Remote effects on the striatal dopamine system after fluid percussion injury. Behavioural Brain Research, 267, 156-172. https://doi.org/10.1016/j.bbr.2014.03.033

Remote effects on the striatal dopamine system after fluid percussion injury. / Huang, Eagle Yi Kung; Tsai, Tung Han; Kuo, Tung Tai; Tsai, Jing Jr; Tsui, Pi Fen; Chou, Yu Ching; Ma, Hsin I.; Chiang, Yung Hsiao; Chen, Yuan Hao.

In: Behavioural Brain Research, Vol. 267, 01.07.2014, p. 156-172.

Research output: Contribution to journalArticle

Huang, EYK, Tsai, TH, Kuo, TT, Tsai, JJ, Tsui, PF, Chou, YC, Ma, HI, Chiang, YH & Chen, YH 2014, 'Remote effects on the striatal dopamine system after fluid percussion injury', Behavioural Brain Research, vol. 267, pp. 156-172. https://doi.org/10.1016/j.bbr.2014.03.033
Huang, Eagle Yi Kung ; Tsai, Tung Han ; Kuo, Tung Tai ; Tsai, Jing Jr ; Tsui, Pi Fen ; Chou, Yu Ching ; Ma, Hsin I. ; Chiang, Yung Hsiao ; Chen, Yuan Hao. / Remote effects on the striatal dopamine system after fluid percussion injury. In: Behavioural Brain Research. 2014 ; Vol. 267. pp. 156-172.
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abstract = "Purpose: To investigate the effects of traumatic brain injury (TBI) on the dopamine system in the brain at different distances from the impaction site, we compared the release, reuptake, metabolism, and release probability of dopamine on the sides of the brain ipsilateral and contralateral to the injury at different time points after varying severities of fluid percussion injuries. Materials and methods: Tonic (1-pulse evoked) and bursting (10-pulse evoked) dopamine release changes in the ipsilateral and contralateral sides of the striatum resulting from mild (2-Pa) and severe (6-Pa) levels of fluid percussion injury were analyzed at the acute (2. h and 24. h), subacute (1 and 2 weeks), and chronic stages (4, 6, and 8 weeks) after injury by using fast scan cyclic voltammetry to measure brain slices. The metabolic rate of striatal dopamine was surveyed using high-performance liquid chromatography. The microglia reaction was analyzed using immunohistochemistry at each stage. Results: In 6-Pa injured animals, for both tonic and bursting dopamine release, reuptake and release probability were suppressed on both the ipsilateral and contralateral sides of the striatum from the acute to the chronic stage. These neuronal activities were also affected at the subacute stage on both sides of the striatum in 2-Pa injured animals. The turnover rate of dopamine was not affected in the 2-Pa injured animals but increased gradually during the chronic stage in the 6-Pa injured group. Conclusion: TBI suppresses dopamine release and reuptake and affects the metabolic rate and release probability of dopamine on the sides of the nigrostriatal system both ipsilateral and contralateral to the injury during both the acute and subacute stages after the injury.",
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AU - Tsai, Tung Han

AU - Kuo, Tung Tai

AU - Tsai, Jing Jr

AU - Tsui, Pi Fen

AU - Chou, Yu Ching

AU - Ma, Hsin I.

AU - Chiang, Yung Hsiao

AU - Chen, Yuan Hao

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N2 - Purpose: To investigate the effects of traumatic brain injury (TBI) on the dopamine system in the brain at different distances from the impaction site, we compared the release, reuptake, metabolism, and release probability of dopamine on the sides of the brain ipsilateral and contralateral to the injury at different time points after varying severities of fluid percussion injuries. Materials and methods: Tonic (1-pulse evoked) and bursting (10-pulse evoked) dopamine release changes in the ipsilateral and contralateral sides of the striatum resulting from mild (2-Pa) and severe (6-Pa) levels of fluid percussion injury were analyzed at the acute (2. h and 24. h), subacute (1 and 2 weeks), and chronic stages (4, 6, and 8 weeks) after injury by using fast scan cyclic voltammetry to measure brain slices. The metabolic rate of striatal dopamine was surveyed using high-performance liquid chromatography. The microglia reaction was analyzed using immunohistochemistry at each stage. Results: In 6-Pa injured animals, for both tonic and bursting dopamine release, reuptake and release probability were suppressed on both the ipsilateral and contralateral sides of the striatum from the acute to the chronic stage. These neuronal activities were also affected at the subacute stage on both sides of the striatum in 2-Pa injured animals. The turnover rate of dopamine was not affected in the 2-Pa injured animals but increased gradually during the chronic stage in the 6-Pa injured group. Conclusion: TBI suppresses dopamine release and reuptake and affects the metabolic rate and release probability of dopamine on the sides of the nigrostriatal system both ipsilateral and contralateral to the injury during both the acute and subacute stages after the injury.

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KW - High-pressure liquid chromatography (HPLC)

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