Release and capture of bioactive oxidized phospholipids and oxidized cholesteryl esters during percutaneous coronary and peripheral arterial interventions in humans

Amir Ravandi, Gregor Leibundgut, Ming Yow Hung, Mitul Patel, Patrick M. Hutchins, Robert C. Murphy, Anand Prasad, Ehtisham Mahmud, Yury I. Miller, Edward A. Dennis, Joseph L. Witztum, Sotirios Tsimikas

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Objectives This study sought to assess whether oxidized lipids are released downstream from obstructive plaques after percutaneous coronary and peripheral interventions using distal protection devices. Background Oxidation of lipoproteins generates multiple bioactive oxidized lipids that affect atherothrombosis and endothelial function. Direct evidence of their role during therapeutic procedures, which may result in no-reflow phenomenon, myocardial infarction, and stroke, is lacking. Methods The presence of specific oxidized lipids was assessed in embolized material captured by distal protection filter devices during uncomplicated saphenous vein graft, carotid, renal, and superficial femoral artery interventions. The presence of oxidized phospholipids (OxPL) and oxidized cholesteryl esters (OxCE) was evaluated in 24 filters using liquid chromatography, tandem mass spectrometry, enzyme-linked immunosorbent assays, and immunostaining. Results Phosphatidylcholine-containing OxPL, including (1-palmitoyl-2-[9-oxo-nonanoyl] PC), representing a major phosphatidylcholine-OxPL molecule quantitated within plaque material, [1-palmitoyl-2-(5-oxo-valeroyl)-sn-glycero-3-phosphocholine], and 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine, were identified in the extracted lipid portion from all vascular beds. Several species of OxCE, such as keto, hydroperoxide, hydroxy, and epoxy cholesteryl ester derivatives from cholesteryl linoleate and cholesteryl arachidonate, were also present. The presence of OxPL was confirmed using enzyme-linked immunoassays and immunohistochemistry of captured material. Conclusions This study documents the direct release and capture of OxPL and OxCE during percutaneous interventions from multiple arterial beds in humans. Entrance of bioactive oxidized lipids into the microcirculation may mediate adverse clinical outcomes during therapeutic procedures.

Original languageEnglish
Pages (from-to)1961-1971
Number of pages11
JournalJournal of the American College of Cardiology
Volume63
Issue number19
DOIs
Publication statusPublished - May 20 2014

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Cholesterol Esters
Phospholipids
Lipids
Phosphorylcholine
Phosphatidylcholines
No-Reflow Phenomenon
Equipment and Supplies
Saphenous Vein
Percutaneous Coronary Intervention
Femoral Artery
Microcirculation
Tandem Mass Spectrometry
Immunoenzyme Techniques
Liquid Chromatography
Hydrogen Peroxide
Lipoproteins
Blood Vessels
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Stroke

Keywords

  • angioplasty
  • lipoproteins
  • oxidized cholesteryl esters
  • oxidized phospholipids

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Release and capture of bioactive oxidized phospholipids and oxidized cholesteryl esters during percutaneous coronary and peripheral arterial interventions in humans. / Ravandi, Amir; Leibundgut, Gregor; Hung, Ming Yow; Patel, Mitul; Hutchins, Patrick M.; Murphy, Robert C.; Prasad, Anand; Mahmud, Ehtisham; Miller, Yury I.; Dennis, Edward A.; Witztum, Joseph L.; Tsimikas, Sotirios.

In: Journal of the American College of Cardiology, Vol. 63, No. 19, 20.05.2014, p. 1961-1971.

Research output: Contribution to journalArticle

Ravandi, A, Leibundgut, G, Hung, MY, Patel, M, Hutchins, PM, Murphy, RC, Prasad, A, Mahmud, E, Miller, YI, Dennis, EA, Witztum, JL & Tsimikas, S 2014, 'Release and capture of bioactive oxidized phospholipids and oxidized cholesteryl esters during percutaneous coronary and peripheral arterial interventions in humans', Journal of the American College of Cardiology, vol. 63, no. 19, pp. 1961-1971. https://doi.org/10.1016/j.jacc.2014.01.055
Ravandi, Amir ; Leibundgut, Gregor ; Hung, Ming Yow ; Patel, Mitul ; Hutchins, Patrick M. ; Murphy, Robert C. ; Prasad, Anand ; Mahmud, Ehtisham ; Miller, Yury I. ; Dennis, Edward A. ; Witztum, Joseph L. ; Tsimikas, Sotirios. / Release and capture of bioactive oxidized phospholipids and oxidized cholesteryl esters during percutaneous coronary and peripheral arterial interventions in humans. In: Journal of the American College of Cardiology. 2014 ; Vol. 63, No. 19. pp. 1961-1971.
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abstract = "Objectives This study sought to assess whether oxidized lipids are released downstream from obstructive plaques after percutaneous coronary and peripheral interventions using distal protection devices. Background Oxidation of lipoproteins generates multiple bioactive oxidized lipids that affect atherothrombosis and endothelial function. Direct evidence of their role during therapeutic procedures, which may result in no-reflow phenomenon, myocardial infarction, and stroke, is lacking. Methods The presence of specific oxidized lipids was assessed in embolized material captured by distal protection filter devices during uncomplicated saphenous vein graft, carotid, renal, and superficial femoral artery interventions. The presence of oxidized phospholipids (OxPL) and oxidized cholesteryl esters (OxCE) was evaluated in 24 filters using liquid chromatography, tandem mass spectrometry, enzyme-linked immunosorbent assays, and immunostaining. Results Phosphatidylcholine-containing OxPL, including (1-palmitoyl-2-[9-oxo-nonanoyl] PC), representing a major phosphatidylcholine-OxPL molecule quantitated within plaque material, [1-palmitoyl-2-(5-oxo-valeroyl)-sn-glycero-3-phosphocholine], and 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine, were identified in the extracted lipid portion from all vascular beds. Several species of OxCE, such as keto, hydroperoxide, hydroxy, and epoxy cholesteryl ester derivatives from cholesteryl linoleate and cholesteryl arachidonate, were also present. The presence of OxPL was confirmed using enzyme-linked immunoassays and immunohistochemistry of captured material. Conclusions This study documents the direct release and capture of OxPL and OxCE during percutaneous interventions from multiple arterial beds in humans. Entrance of bioactive oxidized lipids into the microcirculation may mediate adverse clinical outcomes during therapeutic procedures.",
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AU - Ravandi, Amir

AU - Leibundgut, Gregor

AU - Hung, Ming Yow

AU - Patel, Mitul

AU - Hutchins, Patrick M.

AU - Murphy, Robert C.

AU - Prasad, Anand

AU - Mahmud, Ehtisham

AU - Miller, Yury I.

AU - Dennis, Edward A.

AU - Witztum, Joseph L.

AU - Tsimikas, Sotirios

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N2 - Objectives This study sought to assess whether oxidized lipids are released downstream from obstructive plaques after percutaneous coronary and peripheral interventions using distal protection devices. Background Oxidation of lipoproteins generates multiple bioactive oxidized lipids that affect atherothrombosis and endothelial function. Direct evidence of their role during therapeutic procedures, which may result in no-reflow phenomenon, myocardial infarction, and stroke, is lacking. Methods The presence of specific oxidized lipids was assessed in embolized material captured by distal protection filter devices during uncomplicated saphenous vein graft, carotid, renal, and superficial femoral artery interventions. The presence of oxidized phospholipids (OxPL) and oxidized cholesteryl esters (OxCE) was evaluated in 24 filters using liquid chromatography, tandem mass spectrometry, enzyme-linked immunosorbent assays, and immunostaining. Results Phosphatidylcholine-containing OxPL, including (1-palmitoyl-2-[9-oxo-nonanoyl] PC), representing a major phosphatidylcholine-OxPL molecule quantitated within plaque material, [1-palmitoyl-2-(5-oxo-valeroyl)-sn-glycero-3-phosphocholine], and 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine, were identified in the extracted lipid portion from all vascular beds. Several species of OxCE, such as keto, hydroperoxide, hydroxy, and epoxy cholesteryl ester derivatives from cholesteryl linoleate and cholesteryl arachidonate, were also present. The presence of OxPL was confirmed using enzyme-linked immunoassays and immunohistochemistry of captured material. Conclusions This study documents the direct release and capture of OxPL and OxCE during percutaneous interventions from multiple arterial beds in humans. Entrance of bioactive oxidized lipids into the microcirculation may mediate adverse clinical outcomes during therapeutic procedures.

AB - Objectives This study sought to assess whether oxidized lipids are released downstream from obstructive plaques after percutaneous coronary and peripheral interventions using distal protection devices. Background Oxidation of lipoproteins generates multiple bioactive oxidized lipids that affect atherothrombosis and endothelial function. Direct evidence of their role during therapeutic procedures, which may result in no-reflow phenomenon, myocardial infarction, and stroke, is lacking. Methods The presence of specific oxidized lipids was assessed in embolized material captured by distal protection filter devices during uncomplicated saphenous vein graft, carotid, renal, and superficial femoral artery interventions. The presence of oxidized phospholipids (OxPL) and oxidized cholesteryl esters (OxCE) was evaluated in 24 filters using liquid chromatography, tandem mass spectrometry, enzyme-linked immunosorbent assays, and immunostaining. Results Phosphatidylcholine-containing OxPL, including (1-palmitoyl-2-[9-oxo-nonanoyl] PC), representing a major phosphatidylcholine-OxPL molecule quantitated within plaque material, [1-palmitoyl-2-(5-oxo-valeroyl)-sn-glycero-3-phosphocholine], and 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine, were identified in the extracted lipid portion from all vascular beds. Several species of OxCE, such as keto, hydroperoxide, hydroxy, and epoxy cholesteryl ester derivatives from cholesteryl linoleate and cholesteryl arachidonate, were also present. The presence of OxPL was confirmed using enzyme-linked immunoassays and immunohistochemistry of captured material. Conclusions This study documents the direct release and capture of OxPL and OxCE during percutaneous interventions from multiple arterial beds in humans. Entrance of bioactive oxidized lipids into the microcirculation may mediate adverse clinical outcomes during therapeutic procedures.

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