Relaxation of isolated guinea pig trachea by genistein via inhibition of phosphodiesterase

Ching Chi Lin, Junn Lain Chen, Wun Chang Ko

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We investigated the mechanisms of the relaxant action of genistein, an isoflavone, phytoestrogen and non-specific protein tyrosine kinase inhibitor. Changes in tension of guinea pig tracheal segments were isometrically recorded on a polygraph. Genistein concentration-dependently relaxed histamine (30 μM)-, carbachol (0.2 μM)-, KC1 (30 mM)- and leukotriene D4 (10 nM)-induced precontractions and inhibited cumulative histamine- and carbachol-induced contractions in a non-competitive manner. Genistein also concentration-dependently and non-competitively inhibited the cumulative, Ca2+-induced contractions in the depolarized (K+, 60 mM) trachealis. The remaining nifedipine (10 μM)-induced tension of the histamine (30 μM)-induced precontraction was further relaxed by genistein, suggesting that regardless of whether voltage-dependent calcium channels are blocked genistein may have other mechanisms of relaxant action. These other mechanisms of the relaxant effect of genistein appeared to be epithelium-independent and were not affected by the presence of propranolol (1 μM), 2′5′- dideoxyadenosine (10 ′M), methylene blue (25 μM), glibenclamide (10 μM), Nω-nitro-L-arginine (20 ωM) or α-chymotrypsin (1 U/mL), suggesting that the mechanisms are unrelated to activation of the β-adrenoceptor, of adenylate cyclase, of guanylate cyclase, of adenosine triphosphatesensitive potassium channel opening, of nitric oxide formation or of neuropeptide release, respectively. However, genistein (17.5-35 μM) produced parallel, leftward shifts in the concentration-response curves of forskolin and nitroprusside and significantly increased the pD2 values of these two agonists. Both genistein and 3-isobutyl-1-methylxanthine at various concentrations (10-300 μM) concentration-dependently and significantly inhibited cAMP- and cGMP-phosphodiesterase (PDE) activities of the trachealis. The -log IC50 values of genistein were estimated to be 4.28 and 4.17, respectively. The above results reveal that the mechanisms of the relaxant action of genistein may be due to its non-selective inhibition of both PDE activities.

Original languageEnglish
Pages (from-to)323-329
Number of pages7
JournalPlanta Medica
Volume73
Issue number4
DOIs
Publication statusPublished - Apr 2007

Fingerprint

trachea (vertebrates)
Genistein
genistein
Phosphoric Diester Hydrolases
Trachea
guinea pigs
Guinea Pigs
histamine
Histamine
carbachol
Carbachol
non-specific protein-tyrosine kinase
Dideoxyadenosine
Type 3 Cyclic Nucleotide Phosphodiesterases
glibenclamide
Leukotriene D4
1-Methyl-3-isobutylxanthine
Phytoestrogens
guanylate cyclase
leukotrienes

Keywords

  • Cyclic AMP-phosphodiesterase
  • Cyclic GMP-phosphodiesterase
  • Genistein
  • Guinea pig tracheal relaxation
  • Isoflavone
  • Phosphodiesterase inhibitor

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Relaxation of isolated guinea pig trachea by genistein via inhibition of phosphodiesterase. / Lin, Ching Chi; Chen, Junn Lain; Ko, Wun Chang.

In: Planta Medica, Vol. 73, No. 4, 04.2007, p. 323-329.

Research output: Contribution to journalArticle

Lin, Ching Chi ; Chen, Junn Lain ; Ko, Wun Chang. / Relaxation of isolated guinea pig trachea by genistein via inhibition of phosphodiesterase. In: Planta Medica. 2007 ; Vol. 73, No. 4. pp. 323-329.
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