Reinnervation of muscular targets by nerve regeneration through guidance conduits

Hou-Yu Chiang, Hsiung-Fei Chien, Hsin-Hsin Shen, Jean-Dean Yang, Yu-Hua Chen, Jui-Hsiang Chen, Sung-Tsang Hsieh

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We established histopathologic and neurophysiologic approaches to examine whether different designs of polycaprolactone-engineered nerve conduits (hollow vs. laminated) could promote nerve regeneration as autologous grafts after transection of sciatic nerves. The assessments included morphometric analysis at the level of sciatic nerve, neuromuscular junction (NMJ) and gastrocnemius muscle, and nerve conduction studies on sciatic nerves. Six months after nerve grafting, the nerve fiber density in the hollow-conduit group was similar to that in the autologous-graft group; the laminated-conduit group only achieved ∼20% of these values. The consequences of these differences were reflected in nerve growth into muscular targets; this was demonstrated by combined cholinesterase histochemistry for NMJ and immunohistochemistry for nerve fibers innervating NMJ with an axonal marker, protein gene product 9.5. Hollow conduits had similar index of NMJ innervation as autologous grafts; the values were higher than those of laminated conduits. Among the 3 groups there were same patterns of differences in the cross-sectional area of muscle fibers and amplitudes of compound muscle action potential. These results indicate that hollow conduits were as efficient as autologous grafts to facilitate nerve regeneration, and provide a multidisciplinary approach to quantitatively evaluate muscular reinnervation after nerve injury. Copyright © 2005 by the American Association of Neuropathologists, Inc.
Original languageEnglish
Pages (from-to)576-587
Number of pages12
JournalJournal of Neuropathology and Experimental Neurology
Volume64
Issue number7
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Nerve Regeneration
Neuromuscular Junction
Sciatic Nerve
Transplants
Nerve Fibers
Muscles
Neural Conduction
Cholinesterases
Action Potentials
Skeletal Muscle
Immunohistochemistry
Wounds and Injuries
Growth
Proteins

Keywords

  • Innervation
  • Nerve conduits
  • Nerve graft
  • Nerve regeneration
  • Neuromuscular junctions
  • Polycaprolactone
  • Ubiquitin
  • cholinesterase
  • gene product
  • polycaprolactone
  • protein gene product 9.5
  • unclassified drug
  • animal experiment
  • animal model
  • animal tissue
  • article
  • autograft
  • controlled study
  • gastrocnemius muscle
  • histochemistry
  • histopathology
  • immunohistochemistry
  • male
  • morphometrics
  • muscle action potential
  • muscle cell
  • muscle reinnervation
  • nerve conduction
  • nerve fiber
  • nerve graft
  • nerve growth
  • nerve injury
  • nerve regeneration
  • nerve transection
  • neuromuscular synapse
  • nonhuman
  • priority journal
  • quantitative analysis
  • rat
  • sciatic nerve
  • Action Potentials
  • Animals
  • Biocompatible Materials
  • Cholinesterases
  • Electrophysiology
  • Immunohistochemistry
  • Male
  • Microscopy, Electron, Transmission
  • Muscle, Skeletal
  • Nerve Regeneration
  • Neuromuscular Junction
  • Polyesters
  • Prostheses and Implants
  • Rats
  • Rats, Sprague-Dawley
  • Sciatic Nerve
  • Transplantation, Homologous

Cite this

Chiang, H-Y., Chien, H-F., Shen, H-H., Yang, J-D., Chen, Y-H., Chen, J-H., & Hsieh, S-T. (2005). Reinnervation of muscular targets by nerve regeneration through guidance conduits. Journal of Neuropathology and Experimental Neurology, 64(7), 576-587.

Reinnervation of muscular targets by nerve regeneration through guidance conduits. / Chiang, Hou-Yu; Chien, Hsiung-Fei; Shen, Hsin-Hsin; Yang, Jean-Dean; Chen, Yu-Hua; Chen, Jui-Hsiang; Hsieh, Sung-Tsang.

In: Journal of Neuropathology and Experimental Neurology, Vol. 64, No. 7, 2005, p. 576-587.

Research output: Contribution to journalArticle

Chiang, H-Y, Chien, H-F, Shen, H-H, Yang, J-D, Chen, Y-H, Chen, J-H & Hsieh, S-T 2005, 'Reinnervation of muscular targets by nerve regeneration through guidance conduits', Journal of Neuropathology and Experimental Neurology, vol. 64, no. 7, pp. 576-587.
Chiang, Hou-Yu ; Chien, Hsiung-Fei ; Shen, Hsin-Hsin ; Yang, Jean-Dean ; Chen, Yu-Hua ; Chen, Jui-Hsiang ; Hsieh, Sung-Tsang. / Reinnervation of muscular targets by nerve regeneration through guidance conduits. In: Journal of Neuropathology and Experimental Neurology. 2005 ; Vol. 64, No. 7. pp. 576-587.
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abstract = "We established histopathologic and neurophysiologic approaches to examine whether different designs of polycaprolactone-engineered nerve conduits (hollow vs. laminated) could promote nerve regeneration as autologous grafts after transection of sciatic nerves. The assessments included morphometric analysis at the level of sciatic nerve, neuromuscular junction (NMJ) and gastrocnemius muscle, and nerve conduction studies on sciatic nerves. Six months after nerve grafting, the nerve fiber density in the hollow-conduit group was similar to that in the autologous-graft group; the laminated-conduit group only achieved ∼20{\%} of these values. The consequences of these differences were reflected in nerve growth into muscular targets; this was demonstrated by combined cholinesterase histochemistry for NMJ and immunohistochemistry for nerve fibers innervating NMJ with an axonal marker, protein gene product 9.5. Hollow conduits had similar index of NMJ innervation as autologous grafts; the values were higher than those of laminated conduits. Among the 3 groups there were same patterns of differences in the cross-sectional area of muscle fibers and amplitudes of compound muscle action potential. These results indicate that hollow conduits were as efficient as autologous grafts to facilitate nerve regeneration, and provide a multidisciplinary approach to quantitatively evaluate muscular reinnervation after nerve injury. Copyright {\circledC} 2005 by the American Association of Neuropathologists, Inc.",
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author = "Hou-Yu Chiang and Hsiung-Fei Chien and Hsin-Hsin Shen and Jean-Dean Yang and Yu-Hua Chen and Jui-Hsiang Chen and Sung-Tsang Hsieh",
note = "被引用次數:20 Export Date: 16 March 2016 CODEN: JNENA 通訊地址: Hsieh, S.-T.; Department of Anatomy and Cell Biology, National Taiwan University College of Medicine, No. 1 Jen-Ai Rd., Sec. 1, Taipei 10063, Taiwan; 電子郵件: sthsieh@ntumc.org 化學物質/CAS: cholinesterase, 9001-08-5; polycaprolactone, 24980-41-4, 25248-42-4; aquaplast, caprolactone, 24980-41-4; Biocompatible Materials; Cholinesterases, EC 3.1.1.8; Polyesters 參考文獻: Bisby, M.A., Regeneration of peripheral nervous system axons (1995) The Axons: Structure, Function and Pathophysiology, pp. 553-577. , Waxman SG, Kocsis JD, Stys PK, eds. New York: Oxford University Press; Griffin, J.W., Hoffman, P.N., Degeneration and regeneration in the peripheral nervous system (1993) Peripheral Neuropathy, pp. 361-375. , Dyck PJ, ed. Philadelphia: W.B. Saunders; Hall, S., Nerve repair: A neurobiologist's view (2001) J Hand Surg, 26, pp. 129-136; Brunelli, G.A., Vigasio, A., Brunelli, G.R., Different conduits in peripheral nerve surgery (1994) Microsurgery, 15, pp. 176-178; Evans, G.R., Brandt, K., Widmer, M.S., In vivo evaluation of poly (L-lactic acid) porous conduits for peripheral nerve regeneration (1999) Biomaterials, 20, pp. 1109-1115; Evans, G.R., Challenges to nerve regeneration (2000) Semin Surg Oncol, 19, pp. 312-318; Evans, G.R., Brandt, K., Niederbichler, A.D., Clinical long-term in vivo evaluation of poly (L-lactic acid) porous conduits for peripheral nerve regeneration (2000) J Biomater Sci Polymer Edn, 11, pp. 869-878; Evans, G.R., Peripheral nerve injury: A review and approach to tissue-engineered constructs (2001) Anat Rec, 263, pp. 396-404; Strauch, B., Use of nerve conduits in peripheral nerve repair (2000) Hand Clin, 16, pp. 123-130; Mackinnon, S., Dellon, A.L., Clinical nerve reconstruction with a bioabsorbable polyglycolic acid tube (1990) Plast Reconstr Surg, 85, pp. 419-424; Bender, M.D., Bennett, J.M., Waddell, R.L., Doctor, J.S., Marra, K.G., Multi-channeled biodegradable polymer/cultispher composite nerve guides (2004) Biomaterials, 25, pp. 1269-1278; Cheng, Z., Teoh, S.H., Surface modification of ultra thin poly (epsilon-caprolactone) films using acrylic acid and collagen (2004) Biomaterials, 25, pp. 1991-2000; Fabre, T., Schappacher, M., Dupuy, B., Soum, A., Bertrand-Barat, J., Baquey, C., Study of a (trimethylenecarbonate-co-ε-caprolactone) polymer-Part 2: In vitro cytocompatibility analysis and in vivo EDI cell response of a new nerve guide (2001) Biomaterials, 22, pp. 2951-2958; Koshimune, M., Takamatsu, K., Nakatsuka, H., Inui, K., Yamano, Y., Ikada, Y., Creating bioabsorbable Schwann cell coated conduits through tissue engineering (2003) Biomed Mater Eng, 13, pp. 223-229; Waddell, R.L., Marra, K.G., Collins, K.L., Leung, J.T., Doctor, J.S., Using PC12 cells to evaluate poly (caprolactone) and collagenous microcarriers for application in nerve guide fabrication (2003) Biotechnol Prog, 19, pp. 1767-1774; Rodriguez, F.J., Gomez, N., Perego, G., Navarro, X., Highly permeable polylactide-caprolactone nerve guides enhance peripheral nerve regeneration through long gaps (1999) Biomaterials, 20, pp. 1489-1500; Ceballos, D., Navarro, X., Dubey, N., Wendelschafer-Crabb, G., Kennedy, W.R., Tranquillo, R.T., Magnetically aligned collagen gel filling a collagen nerve guide improves peripheral nerve regeneration (1999) Exp Neurol, 158, pp. 290-300; Dubey, N., Letourneau, P.C., Tranquillo, R.T., Guided neurite elongation and Schwann cell invasion into magnetically aligned collagen in simulated peripheral nerve regeneration (1999) Exp Neurol, 158, pp. 338-350; Rangappa, N., Romero, A., Nelson, K.D., Eberhart, R.C., Smith, G.M., Laminin-coated poly (L-lactide) filaments induce robust neurite growth while providing directional orientation (2000) J Biomed Mater Res, 51, pp. 625-634; Yoshii, S., Oka, M., Collagen filaments as a scaffold for nerve regeneration (2001) J Biomed Mater Res, 56, pp. 400-405; Terada, N., Bjursten, L.M., Papaloizis, M., Lundborg, G., Resorbable filament structures as a scaffold for matrix formation and axonal growth in bioartificial nerve grafts: Long term observations (1997) Restor Neurol Neurosci, 11, pp. 65-69; Varejao, A.S.P., Cabrita, A.M., Meek, M.F., Fornaro, M., Geuna, S., Giacobini-Robecchi, M.G., Morphology of nerve fiber regeneration along a biodegradable poly (DLLA-ε-CL) nerve guide filled with fresh skeletal muscle (2003) Microsurgery, 23, pp. 338-345; Varejao, A.S.P., Cabrita, A.M., Geuna, S., Functional assessment of sciatic nerve recovery: Biodegradable poly (DLLA-ε-CL) nerve guide filled with fresh skeletal muscle (2003) Microsurgery, 23, pp. 346-353; (1985) Guide for the Care and Use of Lab Animals, , Washington, D.C.: US Department of Health and Human Services; Lin, W.M., Hsieh, S.T., Huang, I.T., Griffin, J.W., Chen, W.P., Ultrastructural localization and regulation of protein gene product 9.5 (1997) Neuroreport, 8, pp. 2999-3004; Ko, M.H., Chen, W.P., Lin-Shiau, S.Y., Hsieh, S.T., Age-dependent acrylamide neurotoxicity in mice: Morphology, physiology, and function (1999) Exp Neurol, 158, pp. 37-46; Zhao, Q.Z., Drott, J., Laurell, T., Rat sciatic nerve regeneration through a micromachined silicon chip (1997) Biomaterials, 18, pp. 75-80; Hadlock, T.A., Sundback, C.A., Hunter, D.A., Vacanti, J.P., Cheney, M.L., A new artificial nerve graft containing rolled Schwann cell monolayers (2001) Microsurgery, 21, pp. 96-101; Sondell, M., Lundborg, G., Kanje, M., Vascular endothelial growth factor stimulates Schwann cells invasion and neovascularization of acellular nerve grafts (1999) Brain Res, 846, pp. 219-222; Hobson, M.I., Green, C.J., Terenghi, G., VEGF enhances intraneural angiogenesis and improves nerve regeneration after axotomy (2000) J Anat, 197, pp. 591-605; Tseng, C.Y., Hu, G., Ambron, R.T., Chiu, D.T., Histologic analysis of Schwann cell migration and peripheral nerve regeneration in the autogenous venous nerve conduit (AVNC) (2003) J Reconstr Microsurg, 19, pp. 331-340; Taniuchi, M., Clark, H.B., Schweitzer, J.B., Johnson Jr., E.M., Induction of nerve growth factor receptor in Schwann cells after axotomy (1986) Proc Natl Acad Sci USA, 83, pp. 4094-4098; Taniuchi, M., Clark, H.B., Schweitzer, J.B., Johnson Jr., E.M., Expression of nerve growth factor receptors by Schwann cells of axotomized peripheral nerves: Ultrastructural location, suppression by axonal contact, and binding properties (1988) J Neurosci, 8, pp. 664-681; You, S., Petrov, T., Chung, P.H., Gordon, T., The expression of the low affinity nerve growth factor receptor in long-term denervated Schwann cells (1997) Glia, 20, pp. 87-100; Hall, S.M., The biology of chronically denervated Schwann cells (1999) Ann NY Acad Sci, 883, pp. 215-233; Chamberlain, L.J., Yannas, I.V., Hsu, H.P., Strichatrz, G.R., Spector, M., Collagen-GAG substrate enhances the quality of nerve regeneration through collagen tubes up to level of autograft (1998) Exp Neurol, 154, pp. 315-329; Mligiliche, N.L., Tabata, Y., Kitada, M., Poly lactic acid-caprolactone copolymer tube with a denatured skeletal muscle segment inside as a guide for peripheral nerve regeneration: A morphological and electrophysiological evaluation of the regenerated nerves (2003) Anat Sci Int, 78, pp. 156-161; Chamberlain, L.J., Yannas, I.V., Hsu, H.P., Strichatrz, G.R., Spector, M., Near-terminus axonal structure and function following rat sciatic nerve regeneration through a collagen-GAG matrix in a ten-millimeter gap (2000) J Neurosci Res, 60, pp. 666-677; Matsumoto, K., Ohnishi, K., Kiyotani, T., Peripheral nerve regeneration across an 80-mm gap bridged by a polyglycolic acid (PGA)-collagen tube filled with laminin-coated collagen fibers: A histological and electrophysiological evaluation of regenerated nerves (2000) Brain Res, 868, pp. 315-328; Verdu, E., Navarro, X., Comparison of immunohistochemical and functional reinnervation (1997) Exp Neurol, 146, pp. 187-198; Chiang, H.Y., Chen, C.T., Chien, H.F., Hsieh, S.T., Skin denervation, neuropathology, and neuropathic pain in a laser-induced focal neuropathy (2005) Neurobiol Dis, 18, pp. 40-53",
year = "2005",
language = "English",
volume = "64",
pages = "576--587",
journal = "American Journal of Psychotherapy",
issn = "0002-9564",
publisher = "Lippincott Williams and Wilkins",
number = "7",

}

TY - JOUR

T1 - Reinnervation of muscular targets by nerve regeneration through guidance conduits

AU - Chiang, Hou-Yu

AU - Chien, Hsiung-Fei

AU - Shen, Hsin-Hsin

AU - Yang, Jean-Dean

AU - Chen, Yu-Hua

AU - Chen, Jui-Hsiang

AU - Hsieh, Sung-Tsang

N1 - 被引用次數:20 Export Date: 16 March 2016 CODEN: JNENA 通訊地址: Hsieh, S.-T.; Department of Anatomy and Cell Biology, National Taiwan University College of Medicine, No. 1 Jen-Ai Rd., Sec. 1, Taipei 10063, Taiwan; 電子郵件: sthsieh@ntumc.org 化學物質/CAS: cholinesterase, 9001-08-5; polycaprolactone, 24980-41-4, 25248-42-4; aquaplast, caprolactone, 24980-41-4; Biocompatible Materials; Cholinesterases, EC 3.1.1.8; Polyesters 參考文獻: Bisby, M.A., Regeneration of peripheral nervous system axons (1995) The Axons: Structure, Function and Pathophysiology, pp. 553-577. , Waxman SG, Kocsis JD, Stys PK, eds. New York: Oxford University Press; Griffin, J.W., Hoffman, P.N., Degeneration and regeneration in the peripheral nervous system (1993) Peripheral Neuropathy, pp. 361-375. , Dyck PJ, ed. Philadelphia: W.B. Saunders; Hall, S., Nerve repair: A neurobiologist's view (2001) J Hand Surg, 26, pp. 129-136; Brunelli, G.A., Vigasio, A., Brunelli, G.R., Different conduits in peripheral nerve surgery (1994) Microsurgery, 15, pp. 176-178; Evans, G.R., Brandt, K., Widmer, M.S., In vivo evaluation of poly (L-lactic acid) porous conduits for peripheral nerve regeneration (1999) Biomaterials, 20, pp. 1109-1115; Evans, G.R., Challenges to nerve regeneration (2000) Semin Surg Oncol, 19, pp. 312-318; Evans, G.R., Brandt, K., Niederbichler, A.D., Clinical long-term in vivo evaluation of poly (L-lactic acid) porous conduits for peripheral nerve regeneration (2000) J Biomater Sci Polymer Edn, 11, pp. 869-878; Evans, G.R., Peripheral nerve injury: A review and approach to tissue-engineered constructs (2001) Anat Rec, 263, pp. 396-404; Strauch, B., Use of nerve conduits in peripheral nerve repair (2000) Hand Clin, 16, pp. 123-130; Mackinnon, S., Dellon, A.L., Clinical nerve reconstruction with a bioabsorbable polyglycolic acid tube (1990) Plast Reconstr Surg, 85, pp. 419-424; Bender, M.D., Bennett, J.M., Waddell, R.L., Doctor, J.S., Marra, K.G., Multi-channeled biodegradable polymer/cultispher composite nerve guides (2004) Biomaterials, 25, pp. 1269-1278; Cheng, Z., Teoh, S.H., Surface modification of ultra thin poly (epsilon-caprolactone) films using acrylic acid and collagen (2004) Biomaterials, 25, pp. 1991-2000; Fabre, T., Schappacher, M., Dupuy, B., Soum, A., Bertrand-Barat, J., Baquey, C., Study of a (trimethylenecarbonate-co-ε-caprolactone) polymer-Part 2: In vitro cytocompatibility analysis and in vivo EDI cell response of a new nerve guide (2001) Biomaterials, 22, pp. 2951-2958; Koshimune, M., Takamatsu, K., Nakatsuka, H., Inui, K., Yamano, Y., Ikada, Y., Creating bioabsorbable Schwann cell coated conduits through tissue engineering (2003) Biomed Mater Eng, 13, pp. 223-229; Waddell, R.L., Marra, K.G., Collins, K.L., Leung, J.T., Doctor, J.S., Using PC12 cells to evaluate poly (caprolactone) and collagenous microcarriers for application in nerve guide fabrication (2003) Biotechnol Prog, 19, pp. 1767-1774; Rodriguez, F.J., Gomez, N., Perego, G., Navarro, X., Highly permeable polylactide-caprolactone nerve guides enhance peripheral nerve regeneration through long gaps (1999) Biomaterials, 20, pp. 1489-1500; Ceballos, D., Navarro, X., Dubey, N., Wendelschafer-Crabb, G., Kennedy, W.R., Tranquillo, R.T., Magnetically aligned collagen gel filling a collagen nerve guide improves peripheral nerve regeneration (1999) Exp Neurol, 158, pp. 290-300; Dubey, N., Letourneau, P.C., Tranquillo, R.T., Guided neurite elongation and Schwann cell invasion into magnetically aligned collagen in simulated peripheral nerve regeneration (1999) Exp Neurol, 158, pp. 338-350; Rangappa, N., Romero, A., Nelson, K.D., Eberhart, R.C., Smith, G.M., Laminin-coated poly (L-lactide) filaments induce robust neurite growth while providing directional orientation (2000) J Biomed Mater Res, 51, pp. 625-634; Yoshii, S., Oka, M., Collagen filaments as a scaffold for nerve regeneration (2001) J Biomed Mater Res, 56, pp. 400-405; Terada, N., Bjursten, L.M., Papaloizis, M., Lundborg, G., Resorbable filament structures as a scaffold for matrix formation and axonal growth in bioartificial nerve grafts: Long term observations (1997) Restor Neurol Neurosci, 11, pp. 65-69; Varejao, A.S.P., Cabrita, A.M., Meek, M.F., Fornaro, M., Geuna, S., Giacobini-Robecchi, M.G., Morphology of nerve fiber regeneration along a biodegradable poly (DLLA-ε-CL) nerve guide filled with fresh skeletal muscle (2003) Microsurgery, 23, pp. 338-345; Varejao, A.S.P., Cabrita, A.M., Geuna, S., Functional assessment of sciatic nerve recovery: Biodegradable poly (DLLA-ε-CL) nerve guide filled with fresh skeletal muscle (2003) Microsurgery, 23, pp. 346-353; (1985) Guide for the Care and Use of Lab Animals, , Washington, D.C.: US Department of Health and Human Services; Lin, W.M., Hsieh, S.T., Huang, I.T., Griffin, J.W., Chen, W.P., Ultrastructural localization and regulation of protein gene product 9.5 (1997) Neuroreport, 8, pp. 2999-3004; Ko, M.H., Chen, W.P., Lin-Shiau, S.Y., Hsieh, S.T., Age-dependent acrylamide neurotoxicity in mice: Morphology, physiology, and function (1999) Exp Neurol, 158, pp. 37-46; Zhao, Q.Z., Drott, J., Laurell, T., Rat sciatic nerve regeneration through a micromachined silicon chip (1997) Biomaterials, 18, pp. 75-80; Hadlock, T.A., Sundback, C.A., Hunter, D.A., Vacanti, J.P., Cheney, M.L., A new artificial nerve graft containing rolled Schwann cell monolayers (2001) Microsurgery, 21, pp. 96-101; Sondell, M., Lundborg, G., Kanje, M., Vascular endothelial growth factor stimulates Schwann cells invasion and neovascularization of acellular nerve grafts (1999) Brain Res, 846, pp. 219-222; Hobson, M.I., Green, C.J., Terenghi, G., VEGF enhances intraneural angiogenesis and improves nerve regeneration after axotomy (2000) J Anat, 197, pp. 591-605; Tseng, C.Y., Hu, G., Ambron, R.T., Chiu, D.T., Histologic analysis of Schwann cell migration and peripheral nerve regeneration in the autogenous venous nerve conduit (AVNC) (2003) J Reconstr Microsurg, 19, pp. 331-340; Taniuchi, M., Clark, H.B., Schweitzer, J.B., Johnson Jr., E.M., Induction of nerve growth factor receptor in Schwann cells after axotomy (1986) Proc Natl Acad Sci USA, 83, pp. 4094-4098; Taniuchi, M., Clark, H.B., Schweitzer, J.B., Johnson Jr., E.M., Expression of nerve growth factor receptors by Schwann cells of axotomized peripheral nerves: Ultrastructural location, suppression by axonal contact, and binding properties (1988) J Neurosci, 8, pp. 664-681; You, S., Petrov, T., Chung, P.H., Gordon, T., The expression of the low affinity nerve growth factor receptor in long-term denervated Schwann cells (1997) Glia, 20, pp. 87-100; Hall, S.M., The biology of chronically denervated Schwann cells (1999) Ann NY Acad Sci, 883, pp. 215-233; Chamberlain, L.J., Yannas, I.V., Hsu, H.P., Strichatrz, G.R., Spector, M., Collagen-GAG substrate enhances the quality of nerve regeneration through collagen tubes up to level of autograft (1998) Exp Neurol, 154, pp. 315-329; Mligiliche, N.L., Tabata, Y., Kitada, M., Poly lactic acid-caprolactone copolymer tube with a denatured skeletal muscle segment inside as a guide for peripheral nerve regeneration: A morphological and electrophysiological evaluation of the regenerated nerves (2003) Anat Sci Int, 78, pp. 156-161; Chamberlain, L.J., Yannas, I.V., Hsu, H.P., Strichatrz, G.R., Spector, M., Near-terminus axonal structure and function following rat sciatic nerve regeneration through a collagen-GAG matrix in a ten-millimeter gap (2000) J Neurosci Res, 60, pp. 666-677; Matsumoto, K., Ohnishi, K., Kiyotani, T., Peripheral nerve regeneration across an 80-mm gap bridged by a polyglycolic acid (PGA)-collagen tube filled with laminin-coated collagen fibers: A histological and electrophysiological evaluation of regenerated nerves (2000) Brain Res, 868, pp. 315-328; Verdu, E., Navarro, X., Comparison of immunohistochemical and functional reinnervation (1997) Exp Neurol, 146, pp. 187-198; Chiang, H.Y., Chen, C.T., Chien, H.F., Hsieh, S.T., Skin denervation, neuropathology, and neuropathic pain in a laser-induced focal neuropathy (2005) Neurobiol Dis, 18, pp. 40-53

PY - 2005

Y1 - 2005

N2 - We established histopathologic and neurophysiologic approaches to examine whether different designs of polycaprolactone-engineered nerve conduits (hollow vs. laminated) could promote nerve regeneration as autologous grafts after transection of sciatic nerves. The assessments included morphometric analysis at the level of sciatic nerve, neuromuscular junction (NMJ) and gastrocnemius muscle, and nerve conduction studies on sciatic nerves. Six months after nerve grafting, the nerve fiber density in the hollow-conduit group was similar to that in the autologous-graft group; the laminated-conduit group only achieved ∼20% of these values. The consequences of these differences were reflected in nerve growth into muscular targets; this was demonstrated by combined cholinesterase histochemistry for NMJ and immunohistochemistry for nerve fibers innervating NMJ with an axonal marker, protein gene product 9.5. Hollow conduits had similar index of NMJ innervation as autologous grafts; the values were higher than those of laminated conduits. Among the 3 groups there were same patterns of differences in the cross-sectional area of muscle fibers and amplitudes of compound muscle action potential. These results indicate that hollow conduits were as efficient as autologous grafts to facilitate nerve regeneration, and provide a multidisciplinary approach to quantitatively evaluate muscular reinnervation after nerve injury. Copyright © 2005 by the American Association of Neuropathologists, Inc.

AB - We established histopathologic and neurophysiologic approaches to examine whether different designs of polycaprolactone-engineered nerve conduits (hollow vs. laminated) could promote nerve regeneration as autologous grafts after transection of sciatic nerves. The assessments included morphometric analysis at the level of sciatic nerve, neuromuscular junction (NMJ) and gastrocnemius muscle, and nerve conduction studies on sciatic nerves. Six months after nerve grafting, the nerve fiber density in the hollow-conduit group was similar to that in the autologous-graft group; the laminated-conduit group only achieved ∼20% of these values. The consequences of these differences were reflected in nerve growth into muscular targets; this was demonstrated by combined cholinesterase histochemistry for NMJ and immunohistochemistry for nerve fibers innervating NMJ with an axonal marker, protein gene product 9.5. Hollow conduits had similar index of NMJ innervation as autologous grafts; the values were higher than those of laminated conduits. Among the 3 groups there were same patterns of differences in the cross-sectional area of muscle fibers and amplitudes of compound muscle action potential. These results indicate that hollow conduits were as efficient as autologous grafts to facilitate nerve regeneration, and provide a multidisciplinary approach to quantitatively evaluate muscular reinnervation after nerve injury. Copyright © 2005 by the American Association of Neuropathologists, Inc.

KW - Innervation

KW - Nerve conduits

KW - Nerve graft

KW - Nerve regeneration

KW - Neuromuscular junctions

KW - Polycaprolactone

KW - Ubiquitin

KW - cholinesterase

KW - gene product

KW - polycaprolactone

KW - protein gene product 9.5

KW - unclassified drug

KW - animal experiment

KW - animal model

KW - animal tissue

KW - article

KW - autograft

KW - controlled study

KW - gastrocnemius muscle

KW - histochemistry

KW - histopathology

KW - immunohistochemistry

KW - male

KW - morphometrics

KW - muscle action potential

KW - muscle cell

KW - muscle reinnervation

KW - nerve conduction

KW - nerve fiber

KW - nerve graft

KW - nerve growth

KW - nerve injury

KW - nerve regeneration

KW - nerve transection

KW - neuromuscular synapse

KW - nonhuman

KW - priority journal

KW - quantitative analysis

KW - rat

KW - sciatic nerve

KW - Action Potentials

KW - Animals

KW - Biocompatible Materials

KW - Cholinesterases

KW - Electrophysiology

KW - Immunohistochemistry

KW - Male

KW - Microscopy, Electron, Transmission

KW - Muscle, Skeletal

KW - Nerve Regeneration

KW - Neuromuscular Junction

KW - Polyesters

KW - Prostheses and Implants

KW - Rats

KW - Rats, Sprague-Dawley

KW - Sciatic Nerve

KW - Transplantation, Homologous

M3 - Article

VL - 64

SP - 576

EP - 587

JO - American Journal of Psychotherapy

JF - American Journal of Psychotherapy

SN - 0002-9564

IS - 7

ER -