Regulation of human cardiac myosin heavy chain genes by cyclical mechanical stretch in cultured cardiocytes

K. G. Shyu, J. J. Chen, N. L. Shih, D. L. Wang, H. Chang, W. P. Lien, C. C. Liew

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We examined the effect of cyclical mechanical stretch on the regulation of cardiac myosin heavy chain genes using an isolated neonatal rat cardiocyte culture system. Cultured cardiocytes grown on a flexible membrane were deformed by vacuum to 20% of maximum elongation, at 60 cycles/min in a serum-free medium. Cyclical stretch did not cause myocyte damage as assessed by supernatant LDH measurement and trypan blue exclusion test. The levels of myosin heavy chain (MyHC) mRNA increased as early as 1 h after stretch, reaching 12-fold over the control in 24 h, as shown by Northern blot analysis. However, the proximal 5'-flanking regions of the α- and β-MyHC gene which were linked to chloramphenicol acetyltransferase (CAT) reporter gene did not exhibit enhanced CAT activity following cyclical stretch. Deletion of the chimeric constructs to shorten the 5'-flanking regions of the MyHC genes generated by polymerase chain reaction amplification did not enhance the CAT activity under cyclical stretch. This finding suggests that the stretch-reponse element of the α- and β-MyHC gene promoter is probably not present in the proximal region of either the α- or β-MyHC genes.

Original languageEnglish
Pages (from-to)567-573
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume210
Issue number2
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Cardiac Myosins
Myosin Heavy Chains
Genes
Chloramphenicol O-Acetyltransferase
5' Flanking Region
Trypan Blue
Polymerase chain reaction
Serum-Free Culture Media
Vacuum
Reporter Genes
Cell culture
Northern Blotting
Muscle Cells
Amplification
Rats
Elongation
Membranes
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Regulation of human cardiac myosin heavy chain genes by cyclical mechanical stretch in cultured cardiocytes. / Shyu, K. G.; Chen, J. J.; Shih, N. L.; Wang, D. L.; Chang, H.; Lien, W. P.; Liew, C. C.

In: Biochemical and Biophysical Research Communications, Vol. 210, No. 2, 1995, p. 567-573.

Research output: Contribution to journalArticle

Shyu, K. G. ; Chen, J. J. ; Shih, N. L. ; Wang, D. L. ; Chang, H. ; Lien, W. P. ; Liew, C. C. / Regulation of human cardiac myosin heavy chain genes by cyclical mechanical stretch in cultured cardiocytes. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 210, No. 2. pp. 567-573.
@article{bac69e14694b4eaab379608924e12c6b,
title = "Regulation of human cardiac myosin heavy chain genes by cyclical mechanical stretch in cultured cardiocytes",
abstract = "We examined the effect of cyclical mechanical stretch on the regulation of cardiac myosin heavy chain genes using an isolated neonatal rat cardiocyte culture system. Cultured cardiocytes grown on a flexible membrane were deformed by vacuum to 20{\%} of maximum elongation, at 60 cycles/min in a serum-free medium. Cyclical stretch did not cause myocyte damage as assessed by supernatant LDH measurement and trypan blue exclusion test. The levels of myosin heavy chain (MyHC) mRNA increased as early as 1 h after stretch, reaching 12-fold over the control in 24 h, as shown by Northern blot analysis. However, the proximal 5'-flanking regions of the α- and β-MyHC gene which were linked to chloramphenicol acetyltransferase (CAT) reporter gene did not exhibit enhanced CAT activity following cyclical stretch. Deletion of the chimeric constructs to shorten the 5'-flanking regions of the MyHC genes generated by polymerase chain reaction amplification did not enhance the CAT activity under cyclical stretch. This finding suggests that the stretch-reponse element of the α- and β-MyHC gene promoter is probably not present in the proximal region of either the α- or β-MyHC genes.",
author = "Shyu, {K. G.} and Chen, {J. J.} and Shih, {N. L.} and Wang, {D. L.} and H. Chang and Lien, {W. P.} and Liew, {C. C.}",
year = "1995",
doi = "10.1006/bbrc.1995.1697",
language = "English",
volume = "210",
pages = "567--573",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier B.V.",
number = "2",

}

TY - JOUR

T1 - Regulation of human cardiac myosin heavy chain genes by cyclical mechanical stretch in cultured cardiocytes

AU - Shyu, K. G.

AU - Chen, J. J.

AU - Shih, N. L.

AU - Wang, D. L.

AU - Chang, H.

AU - Lien, W. P.

AU - Liew, C. C.

PY - 1995

Y1 - 1995

N2 - We examined the effect of cyclical mechanical stretch on the regulation of cardiac myosin heavy chain genes using an isolated neonatal rat cardiocyte culture system. Cultured cardiocytes grown on a flexible membrane were deformed by vacuum to 20% of maximum elongation, at 60 cycles/min in a serum-free medium. Cyclical stretch did not cause myocyte damage as assessed by supernatant LDH measurement and trypan blue exclusion test. The levels of myosin heavy chain (MyHC) mRNA increased as early as 1 h after stretch, reaching 12-fold over the control in 24 h, as shown by Northern blot analysis. However, the proximal 5'-flanking regions of the α- and β-MyHC gene which were linked to chloramphenicol acetyltransferase (CAT) reporter gene did not exhibit enhanced CAT activity following cyclical stretch. Deletion of the chimeric constructs to shorten the 5'-flanking regions of the MyHC genes generated by polymerase chain reaction amplification did not enhance the CAT activity under cyclical stretch. This finding suggests that the stretch-reponse element of the α- and β-MyHC gene promoter is probably not present in the proximal region of either the α- or β-MyHC genes.

AB - We examined the effect of cyclical mechanical stretch on the regulation of cardiac myosin heavy chain genes using an isolated neonatal rat cardiocyte culture system. Cultured cardiocytes grown on a flexible membrane were deformed by vacuum to 20% of maximum elongation, at 60 cycles/min in a serum-free medium. Cyclical stretch did not cause myocyte damage as assessed by supernatant LDH measurement and trypan blue exclusion test. The levels of myosin heavy chain (MyHC) mRNA increased as early as 1 h after stretch, reaching 12-fold over the control in 24 h, as shown by Northern blot analysis. However, the proximal 5'-flanking regions of the α- and β-MyHC gene which were linked to chloramphenicol acetyltransferase (CAT) reporter gene did not exhibit enhanced CAT activity following cyclical stretch. Deletion of the chimeric constructs to shorten the 5'-flanking regions of the MyHC genes generated by polymerase chain reaction amplification did not enhance the CAT activity under cyclical stretch. This finding suggests that the stretch-reponse element of the α- and β-MyHC gene promoter is probably not present in the proximal region of either the α- or β-MyHC genes.

UR - http://www.scopus.com/inward/record.url?scp=0029065392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029065392&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1995.1697

DO - 10.1006/bbrc.1995.1697

M3 - Article

VL - 210

SP - 567

EP - 573

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -