Regulation of eosinophil-active cytokine production from human cord blood-derived mast cells

Guha Krishnaswamy, Kenton Hall, George Youngberg, Fred Hossler, David Johnson, William A. Block, Shau Ku Huang, Jim Kelley, David S. Chi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Human mast cells are multifunctional tissue-dwelling cells that play a crucial role in eosinophil-dependent disorders, such as asthma and parasitic diseases, by the secretion of eosinophil-active mediators. Mast cell-derived cytokines, generated in response to cross-linking of the high-affinity IgE receptor, can regulate eosinophil activation, survival, and chemotaxis. In this study, mast cells generated from human cord blood progenitors (stem cells) were studied for eosinophil-active inflammatory cytokine expression. Cord blood-derived mast cells (CBDMC) expressed typical intracellular scroll granules and microvilli-like structures on their cell surfaces, demonstrated the presence of tryptase, and elaborated prostaglandin D2 (PGD2) after cross-linkage of the high-affinity receptor for IgE (FcεRI). CBDMC expressed tumor necrosis factor-α (TNF-α) and the eosinophil-active growth factors, interleukin-5 (IL-5) and granulocyte-macrophage colony-stimulating factor (GM-CSF) after activation. (IL-1β greatly enhanced IgE-dependent production of these cytokines in response to FcεRI cross-linkage, suggesting a role for bystander/phagocytic cells in modulating mast cell function. In contrast, interferon-α (IFN-α) inhibited IL-5 and GM-CSF generation, and the glucocorticoid, dexamethasone (Dex), inhibited production of IL-5 and GM-CSF from CBDMC. A macrophage-mast cell-eosinophil axis may existin vivo that may be susceptible to pharmacologic manipulation.

Original languageEnglish
Pages (from-to)379-388
Number of pages10
JournalJournal of Interferon and Cytokine Research
Volume22
Issue number3
DOIs
Publication statusPublished - Jan 1 2002
Externally publishedYes

Fingerprint

Fetal Blood
Eosinophils
Mast Cells
Cytokines
Interleukin-5
Granulocyte-Macrophage Colony-Stimulating Factor
IgE Receptors
Stem Cells
Prostaglandin D2
Tryptases
Parasitic Diseases
Chemotaxis
Phagocytes
Microvilli
Interleukin-1
Interferons
Immunoglobulin E
Dexamethasone
Glucocorticoids
Blood Cells

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Virology

Cite this

Krishnaswamy, G., Hall, K., Youngberg, G., Hossler, F., Johnson, D., Block, W. A., ... Chi, D. S. (2002). Regulation of eosinophil-active cytokine production from human cord blood-derived mast cells. Journal of Interferon and Cytokine Research, 22(3), 379-388. https://doi.org/10.1089/107999002753675811

Regulation of eosinophil-active cytokine production from human cord blood-derived mast cells. / Krishnaswamy, Guha; Hall, Kenton; Youngberg, George; Hossler, Fred; Johnson, David; Block, William A.; Huang, Shau Ku; Kelley, Jim; Chi, David S.

In: Journal of Interferon and Cytokine Research, Vol. 22, No. 3, 01.01.2002, p. 379-388.

Research output: Contribution to journalArticle

Krishnaswamy, G, Hall, K, Youngberg, G, Hossler, F, Johnson, D, Block, WA, Huang, SK, Kelley, J & Chi, DS 2002, 'Regulation of eosinophil-active cytokine production from human cord blood-derived mast cells', Journal of Interferon and Cytokine Research, vol. 22, no. 3, pp. 379-388. https://doi.org/10.1089/107999002753675811
Krishnaswamy, Guha ; Hall, Kenton ; Youngberg, George ; Hossler, Fred ; Johnson, David ; Block, William A. ; Huang, Shau Ku ; Kelley, Jim ; Chi, David S. / Regulation of eosinophil-active cytokine production from human cord blood-derived mast cells. In: Journal of Interferon and Cytokine Research. 2002 ; Vol. 22, No. 3. pp. 379-388.
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