Regulation of cytokine production by treating with Chinese tonic herbs in human peripheral blood mononuclear and human acute monocytic leukemia cells

Hsien Yu Shin, Yuh Chi Kuo, Feng-Lin Hsu, Yi Wen Mao, Mei Hsien Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Chinese tonic herbs are often used as remedies for the treatment of cancer or immunity related diseases. Cytokine regulation is one of the pathways that modulates the immune system. In the present study, we investigated the effects of commonly used Chinese herbs on cytokine regulation in human peripheral blood mononuclear cells (PBMC) and human acute monocytic leukemia cell line, THP-1. Among the 15 selected Chinese herbs, Paeonia lactiflora exhibited inhibitory effect on phytohemagglutinin (PHA)-activated PBMC proliferation and induced IFN-γ production. We also used a multiplexed flow cytometric bead-based assay to analyze the release of 17 cytokines and reverse transcription polymerase chain reaction (RT-PCR)/quantitative real-time PCR (qRT-PCR) to evaluate gene expression. Cuscuta chinensis induced both interleukin-8 and MIP-1β release in THP-1 cells and influenced mRNA transcription. Dioscorea opposita, Cinnamomum osmophloeum, and Glycyrrhiza uralensis enhanced secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) and influenced GM-CSF gene expression. As such, different Chinese tonic herbs may regulate immunomodulatory functions in human cells via diverse processes such as the modulation of different cytokines and may be used as adjuvant immunity-boosting drugs. The present study is the first to use high-throughput multiplexed flow cytometric method to analyze cytokine regulatory effects of herbal extracts.

Original languageEnglish
Pages (from-to)414-424
Number of pages11
JournalJournal of Food and Drug Analysis
Volume18
Issue number6
Publication statusPublished - Dec 2010

Fingerprint

Leukemia, Monocytic, Acute
leukemia
herbs
cytokines
Cytokines
blood
granulocyte-macrophage colony-stimulating factor
Granulocyte-Macrophage Colony-Stimulating Factor
mononuclear leukocytes
cells
Immunity
Blood Cells
Cuscuta
Cinnamomum
Glycyrrhiza uralensis
Paeonia lactiflora
immunity
Dioscorea oppositifolia
Paeonia
Dioscorea

Keywords

  • Chinese tonic herbs
  • Human peripheral blood mononuclear cells
  • Multiplexed flow cytometric bead-based assay
  • Quantitative real-time PCR
  • RT-PCR
  • THP-1

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

Regulation of cytokine production by treating with Chinese tonic herbs in human peripheral blood mononuclear and human acute monocytic leukemia cells. / Shin, Hsien Yu; Kuo, Yuh Chi; Hsu, Feng-Lin; Mao, Yi Wen; Lee, Mei Hsien.

In: Journal of Food and Drug Analysis, Vol. 18, No. 6, 12.2010, p. 414-424.

Research output: Contribution to journalArticle

@article{cbf2f6fc648342128a555022a66b5fa8,
title = "Regulation of cytokine production by treating with Chinese tonic herbs in human peripheral blood mononuclear and human acute monocytic leukemia cells",
abstract = "Chinese tonic herbs are often used as remedies for the treatment of cancer or immunity related diseases. Cytokine regulation is one of the pathways that modulates the immune system. In the present study, we investigated the effects of commonly used Chinese herbs on cytokine regulation in human peripheral blood mononuclear cells (PBMC) and human acute monocytic leukemia cell line, THP-1. Among the 15 selected Chinese herbs, Paeonia lactiflora exhibited inhibitory effect on phytohemagglutinin (PHA)-activated PBMC proliferation and induced IFN-γ production. We also used a multiplexed flow cytometric bead-based assay to analyze the release of 17 cytokines and reverse transcription polymerase chain reaction (RT-PCR)/quantitative real-time PCR (qRT-PCR) to evaluate gene expression. Cuscuta chinensis induced both interleukin-8 and MIP-1β release in THP-1 cells and influenced mRNA transcription. Dioscorea opposita, Cinnamomum osmophloeum, and Glycyrrhiza uralensis enhanced secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) and influenced GM-CSF gene expression. As such, different Chinese tonic herbs may regulate immunomodulatory functions in human cells via diverse processes such as the modulation of different cytokines and may be used as adjuvant immunity-boosting drugs. The present study is the first to use high-throughput multiplexed flow cytometric method to analyze cytokine regulatory effects of herbal extracts.",
keywords = "Chinese tonic herbs, Human peripheral blood mononuclear cells, Multiplexed flow cytometric bead-based assay, Quantitative real-time PCR, RT-PCR, THP-1",
author = "Shin, {Hsien Yu} and Kuo, {Yuh Chi} and Feng-Lin Hsu and Mao, {Yi Wen} and Lee, {Mei Hsien}",
year = "2010",
month = "12",
language = "English",
volume = "18",
pages = "414--424",
journal = "Journal of Food and Drug Analysis",
issn = "1021-9498",
publisher = "Elsevier Taiwan LLC",
number = "6",

}

TY - JOUR

T1 - Regulation of cytokine production by treating with Chinese tonic herbs in human peripheral blood mononuclear and human acute monocytic leukemia cells

AU - Shin, Hsien Yu

AU - Kuo, Yuh Chi

AU - Hsu, Feng-Lin

AU - Mao, Yi Wen

AU - Lee, Mei Hsien

PY - 2010/12

Y1 - 2010/12

N2 - Chinese tonic herbs are often used as remedies for the treatment of cancer or immunity related diseases. Cytokine regulation is one of the pathways that modulates the immune system. In the present study, we investigated the effects of commonly used Chinese herbs on cytokine regulation in human peripheral blood mononuclear cells (PBMC) and human acute monocytic leukemia cell line, THP-1. Among the 15 selected Chinese herbs, Paeonia lactiflora exhibited inhibitory effect on phytohemagglutinin (PHA)-activated PBMC proliferation and induced IFN-γ production. We also used a multiplexed flow cytometric bead-based assay to analyze the release of 17 cytokines and reverse transcription polymerase chain reaction (RT-PCR)/quantitative real-time PCR (qRT-PCR) to evaluate gene expression. Cuscuta chinensis induced both interleukin-8 and MIP-1β release in THP-1 cells and influenced mRNA transcription. Dioscorea opposita, Cinnamomum osmophloeum, and Glycyrrhiza uralensis enhanced secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) and influenced GM-CSF gene expression. As such, different Chinese tonic herbs may regulate immunomodulatory functions in human cells via diverse processes such as the modulation of different cytokines and may be used as adjuvant immunity-boosting drugs. The present study is the first to use high-throughput multiplexed flow cytometric method to analyze cytokine regulatory effects of herbal extracts.

AB - Chinese tonic herbs are often used as remedies for the treatment of cancer or immunity related diseases. Cytokine regulation is one of the pathways that modulates the immune system. In the present study, we investigated the effects of commonly used Chinese herbs on cytokine regulation in human peripheral blood mononuclear cells (PBMC) and human acute monocytic leukemia cell line, THP-1. Among the 15 selected Chinese herbs, Paeonia lactiflora exhibited inhibitory effect on phytohemagglutinin (PHA)-activated PBMC proliferation and induced IFN-γ production. We also used a multiplexed flow cytometric bead-based assay to analyze the release of 17 cytokines and reverse transcription polymerase chain reaction (RT-PCR)/quantitative real-time PCR (qRT-PCR) to evaluate gene expression. Cuscuta chinensis induced both interleukin-8 and MIP-1β release in THP-1 cells and influenced mRNA transcription. Dioscorea opposita, Cinnamomum osmophloeum, and Glycyrrhiza uralensis enhanced secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) and influenced GM-CSF gene expression. As such, different Chinese tonic herbs may regulate immunomodulatory functions in human cells via diverse processes such as the modulation of different cytokines and may be used as adjuvant immunity-boosting drugs. The present study is the first to use high-throughput multiplexed flow cytometric method to analyze cytokine regulatory effects of herbal extracts.

KW - Chinese tonic herbs

KW - Human peripheral blood mononuclear cells

KW - Multiplexed flow cytometric bead-based assay

KW - Quantitative real-time PCR

KW - RT-PCR

KW - THP-1

UR - http://www.scopus.com/inward/record.url?scp=79952482472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952482472&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:79952482472

VL - 18

SP - 414

EP - 424

JO - Journal of Food and Drug Analysis

JF - Journal of Food and Drug Analysis

SN - 1021-9498

IS - 6

ER -