Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle.

H. H. Lee, W. H. Chiang, S. H. Chiang, Y. C. Liu, J. Hwang, S. Y. Ng

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen (PCNA) are three important cell cycle regulatory proteins. Recently, their promoters have been isolated, thus facilitating molecular analysis of transcriptional control mechanisms of these genes. Transcription of these three promoters in stable K562 transfectants during different cell cycle phases was analyzed after cell cycle synchronization. About 1 kb of 5' flanking region from either cyclin D1 or DNA topoisomerase I gene is sufficient to confer G1- or S-phase-specific transcription activity to chloramphenicol acetyltransferase (CAT) reporter genes, respectively. In contrast, 2.8 kb of 5' flanking sequences from the PCNA gene led to constitutive transcription, but the inclusion of a segment of the PCNA gene first intron, which contains evolutionarily conserved sequences, could enhance transcription in G1/S-enriched nuclei. This PCNA intron region contains a binding site recognized by the transcription factor E2F. To test whether this site is functional, we cotransfected PCNA-CAT genes with E2F-1 and DP-1 expression plasmids. Expression of the E2F-1/DP-1 heterodimer activated the CAT gene with the PCNA intron. Therefore, this intron region, involved in transcriptional activation at the cell cycle G1/S boundary, is also E2F inducible.

Original languageEnglish
Pages (from-to)95-109
Number of pages15
JournalGene Expression
Volume4
Issue number3
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Type I DNA Topoisomerase
Cyclin D1
Proliferating Cell Nuclear Antigen
Cell Cycle
Introns
Chloramphenicol O-Acetyltransferase
Genes
5' Flanking Region
E2F Transcription Factors
Cell Cycle Proteins
Conserved Sequence
S Phase
Reporter Genes
Transcriptional Activation
Plasmids
Binding Sites

ASJC Scopus subject areas

  • Genetics

Cite this

Lee, H. H., Chiang, W. H., Chiang, S. H., Liu, Y. C., Hwang, J., & Ng, S. Y. (1995). Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle. Gene Expression, 4(3), 95-109.

Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle. / Lee, H. H.; Chiang, W. H.; Chiang, S. H.; Liu, Y. C.; Hwang, J.; Ng, S. Y.

In: Gene Expression, Vol. 4, No. 3, 1995, p. 95-109.

Research output: Contribution to journalArticle

Lee, HH, Chiang, WH, Chiang, SH, Liu, YC, Hwang, J & Ng, SY 1995, 'Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle.', Gene Expression, vol. 4, no. 3, pp. 95-109.
Lee, H. H. ; Chiang, W. H. ; Chiang, S. H. ; Liu, Y. C. ; Hwang, J. ; Ng, S. Y. / Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle. In: Gene Expression. 1995 ; Vol. 4, No. 3. pp. 95-109.
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