Regorafenib induces apoptosis and inhibits metastatic potential of human bladder carcinoma cells

Fei Ting Hsu, Cho Chin Sun, Chia Hsing Wu, Yen Ju Lee, Chih Hung Chiang, Wei Shu Wang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The aim of the present study was to verify the effects of regorafenib on apoptosis and metastatic potential in TSGH 8301 human bladder carcinoma cells in vitro. Cells were treated with different concentration of regorafenib for different periods of time. Effects of regorafenib on cell viability, apoptosis pathways, metastatic potential, and expression of metastatic and anti-apoptotic proteins were evaluated with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay, flow cytometry, cell migration and invasion assay, and western blotting. We found regorafenib significantly reduced cell viability, cell migration and invasion, and expression of metastatic and anti-apoptotic proteins. In addition, regorafenib significantly induced accumulation of sub-G1 phase cells, loss of mitochondrial membrane potential, and expression of active caspase-3 and caspase-8. These results show that regorafenib not only induces apoptosis, but also inhibits metastatic potential in bladder cancer TSGH 8301 cells in vitro.

Original languageEnglish
Pages (from-to)4919-4926
Number of pages8
JournalAnticancer Research
Volume37
Issue number9
DOIs
Publication statusPublished - Sep 1 2017

Keywords

  • Apoptosis
  • Human bladder carcinoma
  • Metastatic potential
  • Regorafenib

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Hsu, F. T., Sun, C. C., Wu, C. H., Lee, Y. J., Chiang, C. H., & Wang, W. S. (2017). Regorafenib induces apoptosis and inhibits metastatic potential of human bladder carcinoma cells. Anticancer Research, 37(9), 4919-4926. https://doi.org/10.21873/anticanres.11901