Regional heterogeneity in immunoreactive macrophages/microglia in the rat pineal gland

Ya Fen Jiang-Shieh, Ching Hsiang Wu, Min Lin Chang, Jeng Yung Shieh, Chen Yuan Wen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Using specific macrophage antibodies (OX-42, OX-6, ED-1 and ED-2), this study examined the distribution of macrophages/microglia in the pineal gland of adult rats. Except for ED-2, all antibodies labeled distinct subpopulations of macrophages/microglia in the gland; ED-2 labeling was hardly detectable. The quantitative study showed that the pineal macrophages/microglia (PMM) expressing complement type 3 receptors (OX-42) were more numerous than those expressing the major histocompatibility complex class II antigen (OX-6) or unknown cytoplasmic/lysosomal antigens (ED-1). The PMM were ubiquitous, especially the OX-42 labeled cells which were distributed from the dorsal to the ventral aspect of the gland. The macrophages/microglia labeled with OX-6 or ED-1 were localized mainly in the intermediate portion of the pineal gland. Immunolabeled cells were sparsely distributed in the distal portion of the pineal gland. A notable feature was that the OX-6 labeled macrophages/microglia showed a proximal-distal gradient in cell density. Another interesting feature was the occurrence of prominent cell aggregations around the larger blood vessels. These cells were mostly round and exhibited different immunoreactivity. Confocal microscopic study with triple immunolabeling further revealed that individual PMM cell possessed two or more different antigens (ED-1+/OX-6+, OX-42+/OX-6+ or OX-42+/ED-1+). Remarkably, a large population co-expressed ED-1+/OX-6+/OX-42+. The present results show that the expression of immunoreactive molecules in PMM varies in topographical distribution of the cells. It is suggested that this may be linked to their immunoregulatory functions in the gland.

Original languageEnglish
Pages (from-to)45-53
Number of pages9
JournalJournal of Pineal Research
Volume35
Issue number1
DOIs
Publication statusPublished - Aug 2003
Externally publishedYes

Fingerprint

Pineal Gland
Microglia
Macrophages
Macrophage-1 Antigen
Antigens
Cell Aggregation
Antibodies
Histocompatibility Antigens Class II
Major Histocompatibility Complex
Blood Vessels
Cell Count

Keywords

  • Heterogeneity
  • Immunomolecules
  • Macrophage
  • Microglia
  • Pineal gland
  • Rat

ASJC Scopus subject areas

  • Endocrinology

Cite this

Regional heterogeneity in immunoreactive macrophages/microglia in the rat pineal gland. / Jiang-Shieh, Ya Fen; Wu, Ching Hsiang; Chang, Min Lin; Shieh, Jeng Yung; Wen, Chen Yuan.

In: Journal of Pineal Research, Vol. 35, No. 1, 08.2003, p. 45-53.

Research output: Contribution to journalArticle

Jiang-Shieh, Ya Fen ; Wu, Ching Hsiang ; Chang, Min Lin ; Shieh, Jeng Yung ; Wen, Chen Yuan. / Regional heterogeneity in immunoreactive macrophages/microglia in the rat pineal gland. In: Journal of Pineal Research. 2003 ; Vol. 35, No. 1. pp. 45-53.
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