Reduction of Matrix Metalloproteinase-3 Expression in Rheumatoid Arthritis Rat Joints by Light-emitting Diode

Shu-Guei Wang, Noboru Kuboyama, Sheng-Yang Lee, Yoshimitsu Abiko

Research output: Contribution to journalArticle

Abstract

Rheumatoid arthritis (RA) is a systemic autoimmune disease that involves inflammation and joint pain and causes bone resorption. Irradiation with light-emitting diodes (LEDs) is being evaluated for the treatment of RA, but the underlying molecular mechanisms are unclear. To determine whether anti-inflammatory effects are provided, LED irradiation was provided for rats with collagen-induced RA (CIA), and total RNA was isolated from the synovial tissues of CIA rat joints. Gene expression profiles were then monitored by DNA microarray. LED irradiation decreased the swelling in CIA rat knee joints. DNA microarray analysis demonstrated that expression of many genes was altered in RA, including increased MMP3 mRNA levels, while MMP3 mRNA levels were reduced by LED irradiation. These reductions in MMP3 mRNA levels by LED irradiation were confirmed by RT-PCR and real-time PCR. Immunohistochemical analysis demonstrated strong staining for MMP3 in the CIA group, and LED irradiation significantly reduced this staining. Clinical features of reduced swelling in CIA knee joints suggest that LED irradiation might be useful as RA therapy. Since MMP3 has been identified as an important factor in the pathogenesis of RA, reducing MMP3 expression by LED irradiation might offer an important mechanism for reducing inflammation in RA.
Original languageEnglish
Pages (from-to)22-29
Number of pages8
JournalInternational Journal of Oral-Medical Sciences
Volume11
Issue number1
Publication statusPublished - 2012

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Matrix Metalloproteinase 3
Rheumatoid Arthritis
Joints
Light
Collagen
Knee Joint
Oligonucleotide Array Sequence Analysis
Messenger RNA
Staining and Labeling
Inflammation
Experimental Arthritis
Arthralgia
Microarray Analysis
Bone Resorption
Transcriptome
Autoimmune Diseases
Real-Time Polymerase Chain Reaction
Anti-Inflammatory Agents
RNA
Gene Expression

Keywords

  • rat joint; MMP3
  • light-emitting diode (LED)
  • rheumatoid arthritis

Cite this

Reduction of Matrix Metalloproteinase-3 Expression in Rheumatoid Arthritis Rat Joints by Light-emitting Diode. / Wang, Shu-Guei; Kuboyama, Noboru; Lee, Sheng-Yang; Abiko, Yoshimitsu.

In: International Journal of Oral-Medical Sciences, Vol. 11, No. 1, 2012, p. 22-29.

Research output: Contribution to journalArticle

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N2 - Rheumatoid arthritis (RA) is a systemic autoimmune disease that involves inflammation and joint pain and causes bone resorption. Irradiation with light-emitting diodes (LEDs) is being evaluated for the treatment of RA, but the underlying molecular mechanisms are unclear. To determine whether anti-inflammatory effects are provided, LED irradiation was provided for rats with collagen-induced RA (CIA), and total RNA was isolated from the synovial tissues of CIA rat joints. Gene expression profiles were then monitored by DNA microarray. LED irradiation decreased the swelling in CIA rat knee joints. DNA microarray analysis demonstrated that expression of many genes was altered in RA, including increased MMP3 mRNA levels, while MMP3 mRNA levels were reduced by LED irradiation. These reductions in MMP3 mRNA levels by LED irradiation were confirmed by RT-PCR and real-time PCR. Immunohistochemical analysis demonstrated strong staining for MMP3 in the CIA group, and LED irradiation significantly reduced this staining. Clinical features of reduced swelling in CIA knee joints suggest that LED irradiation might be useful as RA therapy. Since MMP3 has been identified as an important factor in the pathogenesis of RA, reducing MMP3 expression by LED irradiation might offer an important mechanism for reducing inflammation in RA.

AB - Rheumatoid arthritis (RA) is a systemic autoimmune disease that involves inflammation and joint pain and causes bone resorption. Irradiation with light-emitting diodes (LEDs) is being evaluated for the treatment of RA, but the underlying molecular mechanisms are unclear. To determine whether anti-inflammatory effects are provided, LED irradiation was provided for rats with collagen-induced RA (CIA), and total RNA was isolated from the synovial tissues of CIA rat joints. Gene expression profiles were then monitored by DNA microarray. LED irradiation decreased the swelling in CIA rat knee joints. DNA microarray analysis demonstrated that expression of many genes was altered in RA, including increased MMP3 mRNA levels, while MMP3 mRNA levels were reduced by LED irradiation. These reductions in MMP3 mRNA levels by LED irradiation were confirmed by RT-PCR and real-time PCR. Immunohistochemical analysis demonstrated strong staining for MMP3 in the CIA group, and LED irradiation significantly reduced this staining. Clinical features of reduced swelling in CIA knee joints suggest that LED irradiation might be useful as RA therapy. Since MMP3 has been identified as an important factor in the pathogenesis of RA, reducing MMP3 expression by LED irradiation might offer an important mechanism for reducing inflammation in RA.

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