Reduction of Early Growth Response-1 Gene Expression in Osteoblasts by Hydrogen Peroxide

Chia Hua Yang, Ying Li, Wei Jen Chang, Duen Jeng Wang, Sheng Yang Lee, Yoshimitsu Abiko

Research output: Contribution to journalArticle

Abstract

Bone formation steadily declines with age resulting in a loss of bone mass. Reactive oxygen species (ROS) are thought to be major contributors to the aging process. The zinc finger transcription factor, Early Growth Response-1 (Egr-1) is a potential regulator as a transcription factor of many target genes, and plays a role in cell growth, development, and differentiation. However, the effect of ageing on Egr-1 gene expression has not yet been ascertained. To identify which genes have an altered transcription level associated with bone loss by ageing, the pre-osteoblastic cell MC3T3-E1 was treated with H 2O2, and gene expression profiles analyzed with gene chip technology using the Affymetrix GeneChip analysis system (Mouse; 34,000 genes). The expression of many genes in MC3T3-E1 was altered significantly with Egr-1 being decreased by H2O2. The reduction of Egr-1 mRNA levels was successfully confirmed by reversed transcription polymerase chain reaction (RT-PCR) and real-time PCR. Since it has been reported that Egr-1 plays an important role as a transcription factor for growth factor genes which promote cell proliferation and differentiation of preosteoblastic cells, the reduction of Egr-1 gene expression by H2O2 may be involved in the decline of bone formation in the ageing process.

Original languageEnglish
Pages (from-to)31-34
Number of pages4
JournalJournal of Hard Tissue Biology
Volume17
Issue number1
DOIs
Publication statusPublished - 2008

Fingerprint

Osteoblasts
Gene expression
Hydrogen peroxide
Hydrogen Peroxide
Genes
Gene Expression
Transcription factors
Bone
Growth
Aging of materials
Osteogenesis
Transcription
Early Growth Response Protein 1
Transcription Factors
Bone and Bones
Zinc Fingers
Oligonucleotide Array Sequence Analysis
Growth and Development
Transcriptome
Polymerase chain reaction

Keywords

  • Ageing
  • Early growth response-1
  • Osteoblasts

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

Reduction of Early Growth Response-1 Gene Expression in Osteoblasts by Hydrogen Peroxide. / Yang, Chia Hua; Li, Ying; Chang, Wei Jen; Wang, Duen Jeng; Lee, Sheng Yang; Abiko, Yoshimitsu.

In: Journal of Hard Tissue Biology, Vol. 17, No. 1, 2008, p. 31-34.

Research output: Contribution to journalArticle

Yang, Chia Hua ; Li, Ying ; Chang, Wei Jen ; Wang, Duen Jeng ; Lee, Sheng Yang ; Abiko, Yoshimitsu. / Reduction of Early Growth Response-1 Gene Expression in Osteoblasts by Hydrogen Peroxide. In: Journal of Hard Tissue Biology. 2008 ; Vol. 17, No. 1. pp. 31-34.
@article{9cf359e8bf1a44439c043c3b6eba51d8,
title = "Reduction of Early Growth Response-1 Gene Expression in Osteoblasts by Hydrogen Peroxide",
abstract = "Bone formation steadily declines with age resulting in a loss of bone mass. Reactive oxygen species (ROS) are thought to be major contributors to the aging process. The zinc finger transcription factor, Early Growth Response-1 (Egr-1) is a potential regulator as a transcription factor of many target genes, and plays a role in cell growth, development, and differentiation. However, the effect of ageing on Egr-1 gene expression has not yet been ascertained. To identify which genes have an altered transcription level associated with bone loss by ageing, the pre-osteoblastic cell MC3T3-E1 was treated with H 2O2, and gene expression profiles analyzed with gene chip technology using the Affymetrix GeneChip analysis system (Mouse; 34,000 genes). The expression of many genes in MC3T3-E1 was altered significantly with Egr-1 being decreased by H2O2. The reduction of Egr-1 mRNA levels was successfully confirmed by reversed transcription polymerase chain reaction (RT-PCR) and real-time PCR. Since it has been reported that Egr-1 plays an important role as a transcription factor for growth factor genes which promote cell proliferation and differentiation of preosteoblastic cells, the reduction of Egr-1 gene expression by H2O2 may be involved in the decline of bone formation in the ageing process.",
keywords = "Ageing, Early growth response-1, Osteoblasts",
author = "Yang, {Chia Hua} and Ying Li and Chang, {Wei Jen} and Wang, {Duen Jeng} and Lee, {Sheng Yang} and Yoshimitsu Abiko",
year = "2008",
doi = "10.2485/jhtb.17.31",
language = "English",
volume = "17",
pages = "31--34",
journal = "Journal of Hard Tissue Biology",
issn = "1341-7649",
publisher = "Society of Hard Tissue Regenerative Biology",
number = "1",

}

TY - JOUR

T1 - Reduction of Early Growth Response-1 Gene Expression in Osteoblasts by Hydrogen Peroxide

AU - Yang, Chia Hua

AU - Li, Ying

AU - Chang, Wei Jen

AU - Wang, Duen Jeng

AU - Lee, Sheng Yang

AU - Abiko, Yoshimitsu

PY - 2008

Y1 - 2008

N2 - Bone formation steadily declines with age resulting in a loss of bone mass. Reactive oxygen species (ROS) are thought to be major contributors to the aging process. The zinc finger transcription factor, Early Growth Response-1 (Egr-1) is a potential regulator as a transcription factor of many target genes, and plays a role in cell growth, development, and differentiation. However, the effect of ageing on Egr-1 gene expression has not yet been ascertained. To identify which genes have an altered transcription level associated with bone loss by ageing, the pre-osteoblastic cell MC3T3-E1 was treated with H 2O2, and gene expression profiles analyzed with gene chip technology using the Affymetrix GeneChip analysis system (Mouse; 34,000 genes). The expression of many genes in MC3T3-E1 was altered significantly with Egr-1 being decreased by H2O2. The reduction of Egr-1 mRNA levels was successfully confirmed by reversed transcription polymerase chain reaction (RT-PCR) and real-time PCR. Since it has been reported that Egr-1 plays an important role as a transcription factor for growth factor genes which promote cell proliferation and differentiation of preosteoblastic cells, the reduction of Egr-1 gene expression by H2O2 may be involved in the decline of bone formation in the ageing process.

AB - Bone formation steadily declines with age resulting in a loss of bone mass. Reactive oxygen species (ROS) are thought to be major contributors to the aging process. The zinc finger transcription factor, Early Growth Response-1 (Egr-1) is a potential regulator as a transcription factor of many target genes, and plays a role in cell growth, development, and differentiation. However, the effect of ageing on Egr-1 gene expression has not yet been ascertained. To identify which genes have an altered transcription level associated with bone loss by ageing, the pre-osteoblastic cell MC3T3-E1 was treated with H 2O2, and gene expression profiles analyzed with gene chip technology using the Affymetrix GeneChip analysis system (Mouse; 34,000 genes). The expression of many genes in MC3T3-E1 was altered significantly with Egr-1 being decreased by H2O2. The reduction of Egr-1 mRNA levels was successfully confirmed by reversed transcription polymerase chain reaction (RT-PCR) and real-time PCR. Since it has been reported that Egr-1 plays an important role as a transcription factor for growth factor genes which promote cell proliferation and differentiation of preosteoblastic cells, the reduction of Egr-1 gene expression by H2O2 may be involved in the decline of bone formation in the ageing process.

KW - Ageing

KW - Early growth response-1

KW - Osteoblasts

UR - http://www.scopus.com/inward/record.url?scp=70349897361&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349897361&partnerID=8YFLogxK

U2 - 10.2485/jhtb.17.31

DO - 10.2485/jhtb.17.31

M3 - Article

VL - 17

SP - 31

EP - 34

JO - Journal of Hard Tissue Biology

JF - Journal of Hard Tissue Biology

SN - 1341-7649

IS - 1

ER -