Reduced XPC messenger RNA level may predict a poor outcome of patients with nonsmall cell lung cancer

Yi Hui Wu, Ya Wen Cheng, Jinghua Chang Tsai, Tzu Chin Wu, Chih Yi Chen, Huei Lee

Research output: Contribution to journalArticle

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Abstract

BACKGROUND. Homologous deletion of the xeroderma pigmentosum complementary group C (XPCF) repair gene frequently causes lung adenocarcinoma in mice, suggesting that an XPC defect may play a critical role in lung tumorigenesis. The current study attempted to determine whether reduced XPC mRNA levels predict the clinical outcome of lung cancer patients. METHODS. XPC, p27kip (cdk inhibitory protein), and S-phase kinase-associated protein (skp2) levels were evaluated by Western blot analysis in a series of lung cancer cell lines with different invasive abilities. Migration and invasive abilities were measured using a modified Boyden chamber without and with Matrigel, respectively. To test whether XPC affects cell invasive ability, XPC gene protein expression was reduced in low invasive cells by RNA interference (RNAi) and assayed with Boyden chamber. XPC mRNA levels in 126 nonsmall cell lung cancers (NSCLCs) were examined by real-time-reverse-transcriptase polymerase chain reaction (RT-PCR). The prognostic value of XPC mRNA expression was statistically analyzed by the Kaplan-Meier method and Cox proportional hazards regression. RESULTS. The expression of XPC was reduced with increasing invasive potential in CLl-series lung cancer cell lines. When the XPC level was reduced by RNAi, cell migration and invasiveness increased markedly; the increased invasiveness may be caused by decreased expression of p27kip and increased expression of skp2 and E2F transcription factor 1. To determine whether reduced XPC expression was correlated with tumor aggressiveness and poor patient survival, XPC mRNA levels were evaluated by real-time RT-PCR. Kaplan-Meier analysis demonstrated that the median survival of patients with lower XPC mRNA levels was shorter compared with patients with higher XPC mRNA levels (P = .0440). Cox regression analysis further indicated that XPC mRNA level may act as an independent prognostic factor for NSCLC patients (P = .014). CONCLUSIONS. The results of the current study suggest that reduced XPC mRNA level may constitute an independent prognostic factor for NSCLC patients.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalCancer
Volume110
Issue number1
DOIs
Publication statusPublished - Jul 1 2007
Externally publishedYes

Fingerprint

Non-Small Cell Lung Carcinoma
Messenger RNA
Lung Neoplasms
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
S-Phase Kinase-Associated Proteins
E2F1 Transcription Factor
Cell Line
Xeroderma Pigmentosum
Survival
Cyclin-Dependent Kinases
Protein S
Kaplan-Meier Estimate
Cell Movement
Real-Time Polymerase Chain Reaction
Carcinogenesis
Western Blotting
Regression Analysis
Gene Expression
Lung

Keywords

  • Non-small cell lung cancer (NSCLC)
  • Prognosis
  • Real-time-reverse- transcriptase polymerase chain reaction (RT-PCR)
  • Xeroderma pigmentosum complementary group C (XPC) mRNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wu, Y. H., Cheng, Y. W., Tsai, J. C., Wu, T. C., Chen, C. Y., & Lee, H. (2007). Reduced XPC messenger RNA level may predict a poor outcome of patients with nonsmall cell lung cancer. Cancer, 110(1), 215-223. https://doi.org/10.1002/cncr.22743

Reduced XPC messenger RNA level may predict a poor outcome of patients with nonsmall cell lung cancer. / Wu, Yi Hui; Cheng, Ya Wen; Tsai, Jinghua Chang; Wu, Tzu Chin; Chen, Chih Yi; Lee, Huei.

In: Cancer, Vol. 110, No. 1, 01.07.2007, p. 215-223.

Research output: Contribution to journalArticle

Wu, YH, Cheng, YW, Tsai, JC, Wu, TC, Chen, CY & Lee, H 2007, 'Reduced XPC messenger RNA level may predict a poor outcome of patients with nonsmall cell lung cancer', Cancer, vol. 110, no. 1, pp. 215-223. https://doi.org/10.1002/cncr.22743
Wu, Yi Hui ; Cheng, Ya Wen ; Tsai, Jinghua Chang ; Wu, Tzu Chin ; Chen, Chih Yi ; Lee, Huei. / Reduced XPC messenger RNA level may predict a poor outcome of patients with nonsmall cell lung cancer. In: Cancer. 2007 ; Vol. 110, No. 1. pp. 215-223.
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abstract = "BACKGROUND. Homologous deletion of the xeroderma pigmentosum complementary group C (XPCF) repair gene frequently causes lung adenocarcinoma in mice, suggesting that an XPC defect may play a critical role in lung tumorigenesis. The current study attempted to determine whether reduced XPC mRNA levels predict the clinical outcome of lung cancer patients. METHODS. XPC, p27kip (cdk inhibitory protein), and S-phase kinase-associated protein (skp2) levels were evaluated by Western blot analysis in a series of lung cancer cell lines with different invasive abilities. Migration and invasive abilities were measured using a modified Boyden chamber without and with Matrigel, respectively. To test whether XPC affects cell invasive ability, XPC gene protein expression was reduced in low invasive cells by RNA interference (RNAi) and assayed with Boyden chamber. XPC mRNA levels in 126 nonsmall cell lung cancers (NSCLCs) were examined by real-time-reverse-transcriptase polymerase chain reaction (RT-PCR). The prognostic value of XPC mRNA expression was statistically analyzed by the Kaplan-Meier method and Cox proportional hazards regression. RESULTS. The expression of XPC was reduced with increasing invasive potential in CLl-series lung cancer cell lines. When the XPC level was reduced by RNAi, cell migration and invasiveness increased markedly; the increased invasiveness may be caused by decreased expression of p27kip and increased expression of skp2 and E2F transcription factor 1. To determine whether reduced XPC expression was correlated with tumor aggressiveness and poor patient survival, XPC mRNA levels were evaluated by real-time RT-PCR. Kaplan-Meier analysis demonstrated that the median survival of patients with lower XPC mRNA levels was shorter compared with patients with higher XPC mRNA levels (P = .0440). Cox regression analysis further indicated that XPC mRNA level may act as an independent prognostic factor for NSCLC patients (P = .014). CONCLUSIONS. The results of the current study suggest that reduced XPC mRNA level may constitute an independent prognostic factor for NSCLC patients.",
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N2 - BACKGROUND. Homologous deletion of the xeroderma pigmentosum complementary group C (XPCF) repair gene frequently causes lung adenocarcinoma in mice, suggesting that an XPC defect may play a critical role in lung tumorigenesis. The current study attempted to determine whether reduced XPC mRNA levels predict the clinical outcome of lung cancer patients. METHODS. XPC, p27kip (cdk inhibitory protein), and S-phase kinase-associated protein (skp2) levels were evaluated by Western blot analysis in a series of lung cancer cell lines with different invasive abilities. Migration and invasive abilities were measured using a modified Boyden chamber without and with Matrigel, respectively. To test whether XPC affects cell invasive ability, XPC gene protein expression was reduced in low invasive cells by RNA interference (RNAi) and assayed with Boyden chamber. XPC mRNA levels in 126 nonsmall cell lung cancers (NSCLCs) were examined by real-time-reverse-transcriptase polymerase chain reaction (RT-PCR). The prognostic value of XPC mRNA expression was statistically analyzed by the Kaplan-Meier method and Cox proportional hazards regression. RESULTS. The expression of XPC was reduced with increasing invasive potential in CLl-series lung cancer cell lines. When the XPC level was reduced by RNAi, cell migration and invasiveness increased markedly; the increased invasiveness may be caused by decreased expression of p27kip and increased expression of skp2 and E2F transcription factor 1. To determine whether reduced XPC expression was correlated with tumor aggressiveness and poor patient survival, XPC mRNA levels were evaluated by real-time RT-PCR. Kaplan-Meier analysis demonstrated that the median survival of patients with lower XPC mRNA levels was shorter compared with patients with higher XPC mRNA levels (P = .0440). Cox regression analysis further indicated that XPC mRNA level may act as an independent prognostic factor for NSCLC patients (P = .014). CONCLUSIONS. The results of the current study suggest that reduced XPC mRNA level may constitute an independent prognostic factor for NSCLC patients.

AB - BACKGROUND. Homologous deletion of the xeroderma pigmentosum complementary group C (XPCF) repair gene frequently causes lung adenocarcinoma in mice, suggesting that an XPC defect may play a critical role in lung tumorigenesis. The current study attempted to determine whether reduced XPC mRNA levels predict the clinical outcome of lung cancer patients. METHODS. XPC, p27kip (cdk inhibitory protein), and S-phase kinase-associated protein (skp2) levels were evaluated by Western blot analysis in a series of lung cancer cell lines with different invasive abilities. Migration and invasive abilities were measured using a modified Boyden chamber without and with Matrigel, respectively. To test whether XPC affects cell invasive ability, XPC gene protein expression was reduced in low invasive cells by RNA interference (RNAi) and assayed with Boyden chamber. XPC mRNA levels in 126 nonsmall cell lung cancers (NSCLCs) were examined by real-time-reverse-transcriptase polymerase chain reaction (RT-PCR). The prognostic value of XPC mRNA expression was statistically analyzed by the Kaplan-Meier method and Cox proportional hazards regression. RESULTS. The expression of XPC was reduced with increasing invasive potential in CLl-series lung cancer cell lines. When the XPC level was reduced by RNAi, cell migration and invasiveness increased markedly; the increased invasiveness may be caused by decreased expression of p27kip and increased expression of skp2 and E2F transcription factor 1. To determine whether reduced XPC expression was correlated with tumor aggressiveness and poor patient survival, XPC mRNA levels were evaluated by real-time RT-PCR. Kaplan-Meier analysis demonstrated that the median survival of patients with lower XPC mRNA levels was shorter compared with patients with higher XPC mRNA levels (P = .0440). Cox regression analysis further indicated that XPC mRNA level may act as an independent prognostic factor for NSCLC patients (P = .014). CONCLUSIONS. The results of the current study suggest that reduced XPC mRNA level may constitute an independent prognostic factor for NSCLC patients.

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