Reduced number and impaired function of circulating endothelial progenitor cells in patients with abdominal aortic aneurysm

Shih Hsien Sung, Tao Cheng Wu, Jia Shiong Chen, Yung Hsiang Chen, Po Hsun Huang, Shing Jong Lin, Chun Che Shih, Jaw Wen Chen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Aim: Circulating endothelial progenitor cells (EPCs) are associated with coronary artery disease (CAD) and predict its outcome. Although the pathophysiology of abdominal aortic aneurysm (AAA) is different, it shares some risk factors with CAD. Therefore, the correlation between EPCs and AAA was investigated. Methods and results: Seventy-eight subjects (age 77.2±7.8 years) with suspected AAA were prospectively enrolled. Cut-off values (men, 3.5-5.5 cm; women, 3-5 cm) were used to define normal aorta, small AAA, and large AAA on thoraco-abdominal computer tomography. Endothelial function was measured by flow-mediated vasodilation (FMD). Flow cytometry and colony-forming units (CFUs) were used to evaluate circulating EPC numbers. Circulating EPCs were defined as mononuclear cells with low CD45 staining and double-positive staining for KDR, CD34, or CD133. Late out-growth EPCs were cultured from six patients with large AAAs and six age- and sex-matched controls to evaluate proliferation, adhesion, migration, tube formation, and senescence. FMD was significantly lower with large (5.26%±3.11%) and small AAAs (6.31%±3.66%) than in controls (8.88%±4.83%, P=0.008). Both CFUs (normal 38.39±12.99, small AAA 21.22±7.14, large AAA 6.98±1.97; P=0.026) and circulating EPCs (CD34+/KDR+ and CD133+/KDR+) were significantly fewer in AAA patients than in controls. On multivariate analysis, CFUs and circulating EPCs (CD34+/KDR+) were independently, inversely correlated to AAA diameter. Proliferation, adhesion, migration, tube formation, and senescence of late EPCs were significantly impaired in AAA patients. Conclusion: The number and function of EPCs were impaired in AAA patients, suggesting their potential role in AAA.

Original languageEnglish
Pages (from-to)1070-1077
Number of pages8
JournalInternational Journal of Cardiology
Volume168
Issue number2
DOIs
Publication statusPublished - Sep 30 2013
Externally publishedYes

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Abdominal Aortic Aneurysm
Stem Cells
Vasodilation
Endothelial Progenitor Cells
Coronary Artery Disease
Staining and Labeling
Aorta
Flow Cytometry
Multivariate Analysis
Cell Count
Tomography

Keywords

  • Abdominal aortic aneurysm
  • Endothelial function
  • Endothelial progenitor cells

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Reduced number and impaired function of circulating endothelial progenitor cells in patients with abdominal aortic aneurysm. / Sung, Shih Hsien; Wu, Tao Cheng; Chen, Jia Shiong; Chen, Yung Hsiang; Huang, Po Hsun; Lin, Shing Jong; Shih, Chun Che; Chen, Jaw Wen.

In: International Journal of Cardiology, Vol. 168, No. 2, 30.09.2013, p. 1070-1077.

Research output: Contribution to journalArticle

Sung, Shih Hsien ; Wu, Tao Cheng ; Chen, Jia Shiong ; Chen, Yung Hsiang ; Huang, Po Hsun ; Lin, Shing Jong ; Shih, Chun Che ; Chen, Jaw Wen. / Reduced number and impaired function of circulating endothelial progenitor cells in patients with abdominal aortic aneurysm. In: International Journal of Cardiology. 2013 ; Vol. 168, No. 2. pp. 1070-1077.
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abstract = "Aim: Circulating endothelial progenitor cells (EPCs) are associated with coronary artery disease (CAD) and predict its outcome. Although the pathophysiology of abdominal aortic aneurysm (AAA) is different, it shares some risk factors with CAD. Therefore, the correlation between EPCs and AAA was investigated. Methods and results: Seventy-eight subjects (age 77.2±7.8 years) with suspected AAA were prospectively enrolled. Cut-off values (men, 3.5-5.5 cm; women, 3-5 cm) were used to define normal aorta, small AAA, and large AAA on thoraco-abdominal computer tomography. Endothelial function was measured by flow-mediated vasodilation (FMD). Flow cytometry and colony-forming units (CFUs) were used to evaluate circulating EPC numbers. Circulating EPCs were defined as mononuclear cells with low CD45 staining and double-positive staining for KDR, CD34, or CD133. Late out-growth EPCs were cultured from six patients with large AAAs and six age- and sex-matched controls to evaluate proliferation, adhesion, migration, tube formation, and senescence. FMD was significantly lower with large (5.26{\%}±3.11{\%}) and small AAAs (6.31{\%}±3.66{\%}) than in controls (8.88{\%}±4.83{\%}, P=0.008). Both CFUs (normal 38.39±12.99, small AAA 21.22±7.14, large AAA 6.98±1.97; P=0.026) and circulating EPCs (CD34+/KDR+ and CD133+/KDR+) were significantly fewer in AAA patients than in controls. On multivariate analysis, CFUs and circulating EPCs (CD34+/KDR+) were independently, inversely correlated to AAA diameter. Proliferation, adhesion, migration, tube formation, and senescence of late EPCs were significantly impaired in AAA patients. Conclusion: The number and function of EPCs were impaired in AAA patients, suggesting their potential role in AAA.",
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AU - Sung, Shih Hsien

AU - Wu, Tao Cheng

AU - Chen, Jia Shiong

AU - Chen, Yung Hsiang

AU - Huang, Po Hsun

AU - Lin, Shing Jong

AU - Shih, Chun Che

AU - Chen, Jaw Wen

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N2 - Aim: Circulating endothelial progenitor cells (EPCs) are associated with coronary artery disease (CAD) and predict its outcome. Although the pathophysiology of abdominal aortic aneurysm (AAA) is different, it shares some risk factors with CAD. Therefore, the correlation between EPCs and AAA was investigated. Methods and results: Seventy-eight subjects (age 77.2±7.8 years) with suspected AAA were prospectively enrolled. Cut-off values (men, 3.5-5.5 cm; women, 3-5 cm) were used to define normal aorta, small AAA, and large AAA on thoraco-abdominal computer tomography. Endothelial function was measured by flow-mediated vasodilation (FMD). Flow cytometry and colony-forming units (CFUs) were used to evaluate circulating EPC numbers. Circulating EPCs were defined as mononuclear cells with low CD45 staining and double-positive staining for KDR, CD34, or CD133. Late out-growth EPCs were cultured from six patients with large AAAs and six age- and sex-matched controls to evaluate proliferation, adhesion, migration, tube formation, and senescence. FMD was significantly lower with large (5.26%±3.11%) and small AAAs (6.31%±3.66%) than in controls (8.88%±4.83%, P=0.008). Both CFUs (normal 38.39±12.99, small AAA 21.22±7.14, large AAA 6.98±1.97; P=0.026) and circulating EPCs (CD34+/KDR+ and CD133+/KDR+) were significantly fewer in AAA patients than in controls. On multivariate analysis, CFUs and circulating EPCs (CD34+/KDR+) were independently, inversely correlated to AAA diameter. Proliferation, adhesion, migration, tube formation, and senescence of late EPCs were significantly impaired in AAA patients. Conclusion: The number and function of EPCs were impaired in AAA patients, suggesting their potential role in AAA.

AB - Aim: Circulating endothelial progenitor cells (EPCs) are associated with coronary artery disease (CAD) and predict its outcome. Although the pathophysiology of abdominal aortic aneurysm (AAA) is different, it shares some risk factors with CAD. Therefore, the correlation between EPCs and AAA was investigated. Methods and results: Seventy-eight subjects (age 77.2±7.8 years) with suspected AAA were prospectively enrolled. Cut-off values (men, 3.5-5.5 cm; women, 3-5 cm) were used to define normal aorta, small AAA, and large AAA on thoraco-abdominal computer tomography. Endothelial function was measured by flow-mediated vasodilation (FMD). Flow cytometry and colony-forming units (CFUs) were used to evaluate circulating EPC numbers. Circulating EPCs were defined as mononuclear cells with low CD45 staining and double-positive staining for KDR, CD34, or CD133. Late out-growth EPCs were cultured from six patients with large AAAs and six age- and sex-matched controls to evaluate proliferation, adhesion, migration, tube formation, and senescence. FMD was significantly lower with large (5.26%±3.11%) and small AAAs (6.31%±3.66%) than in controls (8.88%±4.83%, P=0.008). Both CFUs (normal 38.39±12.99, small AAA 21.22±7.14, large AAA 6.98±1.97; P=0.026) and circulating EPCs (CD34+/KDR+ and CD133+/KDR+) were significantly fewer in AAA patients than in controls. On multivariate analysis, CFUs and circulating EPCs (CD34+/KDR+) were independently, inversely correlated to AAA diameter. Proliferation, adhesion, migration, tube formation, and senescence of late EPCs were significantly impaired in AAA patients. Conclusion: The number and function of EPCs were impaired in AAA patients, suggesting their potential role in AAA.

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