Reduced expression of circadian clock genes in male alcoholic patients

Ming Chyi Huang, Chia Wei Ho, Chun Hsin Chen, Shing Cheng Liu, Chiao Chicy Chen, Sy Jye Leu

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background: There are clear interactions between chronic alcohol consumption and circadian rhythmicity that is regulated by several circadian clock genes. The altered expressions of these genes have been mainly described in animals. The mammalian master clock in the suprachiasmatic nuclei orchestrates the biological rhythms in peripheral tissues. As peripheral blood mononuclear cells (PBMCs) are the most accessible tissue clinically, we assessed the mRNA levels of these genes in patients with alcohol dependence (AD) undergoing alcohol-withdrawal (AW) treatment.Methods: Twenty-two male patients fulfilled the DSM-IV diagnostic criteria of AD, and 12 comparison healthy control subjects were recruited. The patients with AD were further divided by the presence of delirium tremens (DTs), the most severe form of AW syndrome, into DT group and non-DT group. All the participants received blood withdrawal at 9 am, while the patients with AD had blood collection twice: on the next morning of admission (baseline) and on the seventh day. PBMCs were isolated from whole blood, and the mRNA expression profiles of hClock1, hBmal1, hPer1, hPer2, hCry1, and hCry2 were determined by quantitative real-time PCR.Results: The baseline mRNA levels of the target circadian clock genes were markedly lower in patients with AD than in control subjects. After 1 week of alcohol detoxification, there were very limited restorations of discrete circadian gene expressions. DT group did not differ in the expression patterns of circadian clock genes from non-DT group.Conclusions: This is the first study demonstrating the overall lowering of circadian clock genes among patients with AD. The expression pattern is comparable between patients with and without DTs. Although preliminary with data at only one single time point, the observation of strikingly reduced mRNA levels supports the association between circadian clock gene dysregulation and chronic alcohol intake.

Original languageEnglish
Pages (from-to)1899-1904
Number of pages6
JournalAlcoholism: Clinical and Experimental Research
Volume34
Issue number11
DOIs
Publication statusPublished - Nov 2010

Fingerprint

Circadian Clocks
Alcoholics
Clocks
Alcohol Withdrawal Delirium
Alcoholism
Genes
Alcohols
Blood
Messenger RNA
Periodicity
Blood Cells
Gene Expression
Suprachiasmatic Nucleus
Diagnostic and Statistical Manual of Mental Disorders
Alcohol Drinking
Tissue
Real-Time Polymerase Chain Reaction
Healthy Volunteers
Detoxification
Observation

Keywords

  • Alcohol dependence
  • Alcohol withdrawal
  • Circadian clock gene
  • Circadian rhythm

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Psychiatry and Mental health
  • Toxicology

Cite this

Reduced expression of circadian clock genes in male alcoholic patients. / Huang, Ming Chyi; Ho, Chia Wei; Chen, Chun Hsin; Liu, Shing Cheng; Chen, Chiao Chicy; Leu, Sy Jye.

In: Alcoholism: Clinical and Experimental Research, Vol. 34, No. 11, 11.2010, p. 1899-1904.

Research output: Contribution to journalArticle

Huang, Ming Chyi ; Ho, Chia Wei ; Chen, Chun Hsin ; Liu, Shing Cheng ; Chen, Chiao Chicy ; Leu, Sy Jye. / Reduced expression of circadian clock genes in male alcoholic patients. In: Alcoholism: Clinical and Experimental Research. 2010 ; Vol. 34, No. 11. pp. 1899-1904.
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