Abstract

Teeth are the hardest tissue in the body. The growth of teeth is closely regulated by circadian rhythmicity. Considering that sleep deprivation (SD) is a severe condition that disrupts normal circadian rhythmicity, this study was conducted to determine whether calcium expression (the major element participating in teeth constitution), and dental mass would be significantly impaired following SD. Adolescent rats subjected to 3 weeks of SD were processed for energy dispersive spectrum (EDS), time-of-flight secondary ion mass spectrometry (TOF-SIMS), and micro-computed tomography (micro-CT) analyses. The EDS and TOF-SIMS results indicated that high calcium intensity was detected in both the upper and lower incisors of untreated rats. Micro-CT analysis corresponded closely with spectral data in which an enhanced dental mass was calculated in intact animals. However, following SD, both calcium expression and the dental mass were remarkably decreased to nearly half those of the untreated values. Because SD plays a detrimental role in impairing dental structure, establishing satisfactory sleep behavior would therefore serve as a crucial strategy for preventing or improving prevalent dental dysfunctions.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalApplied Surface Science
Volume345
DOIs
Publication statusPublished - 2015

Fingerprint

Secondary ion mass spectrometry
Tomography
Rats
Calcium
Sleep
Animals
Tissue

Keywords

  • Calcium
  • Dental structure
  • Micro-CT
  • Sleep deprivation
  • Spectrometric analysis
  • TOF-SIMS

ASJC Scopus subject areas

  • Surfaces, Coatings and Films

Cite this

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title = "Reduced dental calcium expression and dental mass in chronic sleep deprived rats: Combined EDS, TOF-SIMS, and micro-CT analysis",
abstract = "Teeth are the hardest tissue in the body. The growth of teeth is closely regulated by circadian rhythmicity. Considering that sleep deprivation (SD) is a severe condition that disrupts normal circadian rhythmicity, this study was conducted to determine whether calcium expression (the major element participating in teeth constitution), and dental mass would be significantly impaired following SD. Adolescent rats subjected to 3 weeks of SD were processed for energy dispersive spectrum (EDS), time-of-flight secondary ion mass spectrometry (TOF-SIMS), and micro-computed tomography (micro-CT) analyses. The EDS and TOF-SIMS results indicated that high calcium intensity was detected in both the upper and lower incisors of untreated rats. Micro-CT analysis corresponded closely with spectral data in which an enhanced dental mass was calculated in intact animals. However, following SD, both calcium expression and the dental mass were remarkably decreased to nearly half those of the untreated values. Because SD plays a detrimental role in impairing dental structure, establishing satisfactory sleep behavior would therefore serve as a crucial strategy for preventing or improving prevalent dental dysfunctions.",
keywords = "Calcium, Dental structure, Micro-CT, Sleep deprivation, Spectrometric analysis, TOF-SIMS",
author = "Kuo, {Yi Jie} and Huang, {Yung Kai} and Chou, {Hsiu Chu} and Pai, {Man Hui} and Lee, {Ai Wei} and Mai, {Fu Der} and Chang, {Hung Ming}",
year = "2015",
doi = "10.1016/j.apsusc.2015.03.132",
language = "English",
volume = "345",
pages = "141--144",
journal = "Applied Surface Science",
issn = "0169-4332",
publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Reduced dental calcium expression and dental mass in chronic sleep deprived rats

T2 - Combined EDS, TOF-SIMS, and micro-CT analysis

AU - Kuo, Yi Jie

AU - Huang, Yung Kai

AU - Chou, Hsiu Chu

AU - Pai, Man Hui

AU - Lee, Ai Wei

AU - Mai, Fu Der

AU - Chang, Hung Ming

PY - 2015

Y1 - 2015

N2 - Teeth are the hardest tissue in the body. The growth of teeth is closely regulated by circadian rhythmicity. Considering that sleep deprivation (SD) is a severe condition that disrupts normal circadian rhythmicity, this study was conducted to determine whether calcium expression (the major element participating in teeth constitution), and dental mass would be significantly impaired following SD. Adolescent rats subjected to 3 weeks of SD were processed for energy dispersive spectrum (EDS), time-of-flight secondary ion mass spectrometry (TOF-SIMS), and micro-computed tomography (micro-CT) analyses. The EDS and TOF-SIMS results indicated that high calcium intensity was detected in both the upper and lower incisors of untreated rats. Micro-CT analysis corresponded closely with spectral data in which an enhanced dental mass was calculated in intact animals. However, following SD, both calcium expression and the dental mass were remarkably decreased to nearly half those of the untreated values. Because SD plays a detrimental role in impairing dental structure, establishing satisfactory sleep behavior would therefore serve as a crucial strategy for preventing or improving prevalent dental dysfunctions.

AB - Teeth are the hardest tissue in the body. The growth of teeth is closely regulated by circadian rhythmicity. Considering that sleep deprivation (SD) is a severe condition that disrupts normal circadian rhythmicity, this study was conducted to determine whether calcium expression (the major element participating in teeth constitution), and dental mass would be significantly impaired following SD. Adolescent rats subjected to 3 weeks of SD were processed for energy dispersive spectrum (EDS), time-of-flight secondary ion mass spectrometry (TOF-SIMS), and micro-computed tomography (micro-CT) analyses. The EDS and TOF-SIMS results indicated that high calcium intensity was detected in both the upper and lower incisors of untreated rats. Micro-CT analysis corresponded closely with spectral data in which an enhanced dental mass was calculated in intact animals. However, following SD, both calcium expression and the dental mass were remarkably decreased to nearly half those of the untreated values. Because SD plays a detrimental role in impairing dental structure, establishing satisfactory sleep behavior would therefore serve as a crucial strategy for preventing or improving prevalent dental dysfunctions.

KW - Calcium

KW - Dental structure

KW - Micro-CT

KW - Sleep deprivation

KW - Spectrometric analysis

KW - TOF-SIMS

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