Redox property of ribonucleotide reductase small subunit M2 and p53R2

Xiyong Liu, Lijun Xue, Yun Yen

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

Human ribonucleotide reductase (RR) small subunits, M2 and P53R2, play key roles in forming RR holoenzyme and supplying nucleotide precursors for DNA replication and repair. Currently, we are studying the redox property, structure, and function of hRRM2 and p53R2. In the cell-free system, p53R2 did not oxidize a reactive oxygen species (ROS) indicator Carboxy-H 2DCFDA, but hRRM2 did. Further studies demonstrated that purified recombinant p53R2 protein has the catalase activity to scavenge H 2O2. Over-expression of p53R2 reduced intracellular ROS and protected the mitochondrial membrane potential against oxidative stress, whereas over-expression of hRRM2 did not result in the collapse of mitochondrial membrane potential. Our findings suggest that p53R2 may play a key role in defending oxidative stress by scavenging ROS, and this antioxidant property is also important for its enzymatic activity.

Original languageEnglish
Title of host publicationAdvanced Protocols in Oxidative Stress I
EditorsDonald Armstrong, Donald Armstrong, Donald Armstrong
Pages195-206
Number of pages12
DOIs
Publication statusPublished - Dec 1 2008
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume477
ISSN (Print)1064-3745

Fingerprint

Oxidation-Reduction
Reactive Oxygen Species
Mitochondrial Membrane Potential
Oxidative Stress
Ribonucleotide Reductases
Holoenzymes
Cell-Free System
DNA Replication
Recombinant Proteins
DNA Repair
Catalase
Nucleotides
Antioxidants
ribonucleotide reductase M2

Keywords

  • Antioxidant
  • p53R2
  • Ribonucleotide reductase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Liu, X., Xue, L., & Yen, Y. (2008). Redox property of ribonucleotide reductase small subunit M2 and p53R2. In D. Armstrong, D. Armstrong, & D. Armstrong (Eds.), Advanced Protocols in Oxidative Stress I (pp. 195-206). (Methods in Molecular Biology; Vol. 477). https://doi.org/10.1007/978-1-60327-517-0_15

Redox property of ribonucleotide reductase small subunit M2 and p53R2. / Liu, Xiyong; Xue, Lijun; Yen, Yun.

Advanced Protocols in Oxidative Stress I. ed. / Donald Armstrong; Donald Armstrong; Donald Armstrong. 2008. p. 195-206 (Methods in Molecular Biology; Vol. 477).

Research output: Chapter in Book/Report/Conference proceedingChapter

Liu, X, Xue, L & Yen, Y 2008, Redox property of ribonucleotide reductase small subunit M2 and p53R2. in D Armstrong, D Armstrong & D Armstrong (eds), Advanced Protocols in Oxidative Stress I. Methods in Molecular Biology, vol. 477, pp. 195-206. https://doi.org/10.1007/978-1-60327-517-0_15
Liu X, Xue L, Yen Y. Redox property of ribonucleotide reductase small subunit M2 and p53R2. In Armstrong D, Armstrong D, Armstrong D, editors, Advanced Protocols in Oxidative Stress I. 2008. p. 195-206. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60327-517-0_15
Liu, Xiyong ; Xue, Lijun ; Yen, Yun. / Redox property of ribonucleotide reductase small subunit M2 and p53R2. Advanced Protocols in Oxidative Stress I. editor / Donald Armstrong ; Donald Armstrong ; Donald Armstrong. 2008. pp. 195-206 (Methods in Molecular Biology).
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