Recurrent BRAF Gene Fusions in a Subset of Pediatric Spindle Cell Sarcomas: Expanding the Genetic Spectrum of Tumors with Overlapping Features with Infantile Fibrosarcoma

Yu-Chien Kao, Christopher D.M. Fletcher, Rita Alaggio, Leonard Wexler, Lei Zhang, Yun Shao Sung, Dicle Orhan, Wei Chin Chang, David Swanson, Brendan C. Dickson, Cristina R. Antonescu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Infantile fibrosarcomas (IFS) represent a distinct group of soft tissue tumors occurring in patients under 2 years of age and most commonly involving the extremities. Most IFS show recurrent ETV6-NTRK3 gene fusions, sensitivity to chemotherapy, and an overall favorable clinical outcome. However, outside these well-defined pathologic features, no studies have investigated IFS lacking ETV6-NTRK3 fusions, or tumors with the morphology resembling IFS in older children. This study was triggered by the identification of a novel SEPT7-BRAF fusion in an unclassified retroperitoneal spindle cell sarcoma in a 16-year-old female by targeted RNA sequencing. Fluorescence in situ hybridization screening of 9 additional tumors with similar phenotype and lacking ETV6-NTRK3 identified 4 additional cases with BRAF gene rearrangements in the pelvic cavity (n=2), paraspinal region (n=1), and thigh (n=1) of young children (0 to 3 y old). Histologically, 4 cases including the index case shared a fascicular growth of packed monomorphic spindle cells, with uniform nuclei and fine chromatin, and a dilated branching vasculature; while the remaining case was composed of compact cellular sheets of short spindle to ovoid cells. In addition, a minor small blue round cell component was present in 1 case. Mitotic activity ranged from 1 to 9/10 high power fields. Immunohistochemical stains were nonspecific, with only focal smooth muscle actin staining demonstrated in 3 cases tested. Of the remaining 5 BRAF negative cases, further RNA sequencing identified 1 case with EML4-NTRK3 in an 1-year-old boy with a foot IFS, and a second case with TPM3-NTRK1 fusion in a 7-week-old infant with a retroperitoneal lesion. Our findings of recurrent BRAF gene rearrangements in tumors showing morphologic overlap with IFS expand the genetic spectrum of fusion-positive spindle cell sarcomas, to include unusual presentations, such as older children and adolescents and predilection for axial location, thereby opening new opportunities for kinase-targeted therapeutic intervention.

Original languageEnglish
Pages (from-to)28-38
Number of pages11
JournalAmerican Journal of Surgical Pathology
Volume42
Issue number1
DOIs
Publication statusPublished - Jan 1 2018
Externally publishedYes

Fingerprint

Fibrosarcoma
Gene Fusion
Sarcoma
Pediatrics
RNA Sequence Analysis
Neoplasms
Gene Rearrangement
Cellular Structures
Thigh
Fluorescence In Situ Hybridization
Chromatin
Smooth Muscle
Actins
Foot
Coloring Agents
Phosphotransferases
Extremities
Staining and Labeling
Phenotype
Drug Therapy

Keywords

  • BRAF
  • fibrosarcoma
  • fusions
  • infantile fibrosarcoma
  • NTRK1
  • NTRK3

ASJC Scopus subject areas

  • Anatomy
  • Surgery
  • Pathology and Forensic Medicine

Cite this

Recurrent BRAF Gene Fusions in a Subset of Pediatric Spindle Cell Sarcomas : Expanding the Genetic Spectrum of Tumors with Overlapping Features with Infantile Fibrosarcoma. / Kao, Yu-Chien; Fletcher, Christopher D.M.; Alaggio, Rita; Wexler, Leonard; Zhang, Lei; Sung, Yun Shao; Orhan, Dicle; Chang, Wei Chin; Swanson, David; Dickson, Brendan C.; Antonescu, Cristina R.

In: American Journal of Surgical Pathology, Vol. 42, No. 1, 01.01.2018, p. 28-38.

Research output: Contribution to journalArticle

Kao, Yu-Chien ; Fletcher, Christopher D.M. ; Alaggio, Rita ; Wexler, Leonard ; Zhang, Lei ; Sung, Yun Shao ; Orhan, Dicle ; Chang, Wei Chin ; Swanson, David ; Dickson, Brendan C. ; Antonescu, Cristina R. / Recurrent BRAF Gene Fusions in a Subset of Pediatric Spindle Cell Sarcomas : Expanding the Genetic Spectrum of Tumors with Overlapping Features with Infantile Fibrosarcoma. In: American Journal of Surgical Pathology. 2018 ; Vol. 42, No. 1. pp. 28-38.
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abstract = "Infantile fibrosarcomas (IFS) represent a distinct group of soft tissue tumors occurring in patients under 2 years of age and most commonly involving the extremities. Most IFS show recurrent ETV6-NTRK3 gene fusions, sensitivity to chemotherapy, and an overall favorable clinical outcome. However, outside these well-defined pathologic features, no studies have investigated IFS lacking ETV6-NTRK3 fusions, or tumors with the morphology resembling IFS in older children. This study was triggered by the identification of a novel SEPT7-BRAF fusion in an unclassified retroperitoneal spindle cell sarcoma in a 16-year-old female by targeted RNA sequencing. Fluorescence in situ hybridization screening of 9 additional tumors with similar phenotype and lacking ETV6-NTRK3 identified 4 additional cases with BRAF gene rearrangements in the pelvic cavity (n=2), paraspinal region (n=1), and thigh (n=1) of young children (0 to 3 y old). Histologically, 4 cases including the index case shared a fascicular growth of packed monomorphic spindle cells, with uniform nuclei and fine chromatin, and a dilated branching vasculature; while the remaining case was composed of compact cellular sheets of short spindle to ovoid cells. In addition, a minor small blue round cell component was present in 1 case. Mitotic activity ranged from 1 to 9/10 high power fields. Immunohistochemical stains were nonspecific, with only focal smooth muscle actin staining demonstrated in 3 cases tested. Of the remaining 5 BRAF negative cases, further RNA sequencing identified 1 case with EML4-NTRK3 in an 1-year-old boy with a foot IFS, and a second case with TPM3-NTRK1 fusion in a 7-week-old infant with a retroperitoneal lesion. Our findings of recurrent BRAF gene rearrangements in tumors showing morphologic overlap with IFS expand the genetic spectrum of fusion-positive spindle cell sarcomas, to include unusual presentations, such as older children and adolescents and predilection for axial location, thereby opening new opportunities for kinase-targeted therapeutic intervention.",
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