Reconstruction of human α thalassemia-2 genotypes in monkey cells

Wei Shau Hu, Che Kun James Shen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The human adult a globin genes, α2 and αl, are contained within two tandemly arranged duplication units. Each unit spans 4 kb of DNA, and contains three homology blocks (X, Y, Z) separated by non-homologous sequences. Segmental DNA recombination processes between the two units have resulted in high frequencies of two types of deletions in certain human populations, each deletion removing one a globin gene from chromosome 16 (α-thalassemia 2). In order to study the molecular mechanisms of α-thalassemia 2, and of homologous DNA recombination in general in mammalian cells, we have reconstructed these two α-thalassemia 2 genotypes in monkey cells. The two duplication units have been cloned in an SV40 origin-containing vector, and transfected into COS 7 cells. Newly replicated plasmid DNA was isolated and analyzed by Southern blot hybridization. Homologous DNA recombination occurs with high frequencies (10-20% per kb of homology), and this generates both types of α-thalassemia 2 deletions on the episomes in the monkey cells. Removal of the 5′ end of either one, or both, of the X blocks prior to DNA transfection affects the relative frequencies of the two α-thalassemia 2 genotypes in a novel way. We consider and discuss these results in terms of several alternative models. Our data suggest the existence of hot spot(s) for initiation of homologous DNA recombination, or recombination promoting element(s), in a specific region of the human adult a globin locus. A DNA sequence that defines the boundaries of the two duplication units, and has been implicated in the initiation of gene conversion of the two X blocks, is contained within this region.

Original languageEnglish
Pages (from-to)2989-3008
Number of pages20
JournalNucleic Acids Research
Volume15
Issue number7
DOIs
Publication statusPublished - Apr 10 1987
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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