Recombinant protein composed of Pseudomonas exotoxin A, outer membrane proteins I and F as vaccine against P. aeruginosa infection

T. Y. Chen, H. F. Shang, T. L. Chen, C. P. Lin, C. F. Hui, J. Hwang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have constructed a chimeric protein composed of the receptor binding and membrane translocation domains of Pseudomonas exotoxin A (PE) with the outer membrane proteins I and F, together designated as PELF. The potential of PEIF as a vaccine against Pseudomonas infection was evaluated in BALB/c mice and New Zealand white rabbits. We examined titers of anti-PE and anti- OprF antibodies, and the ability both to neutralize PE cytotoxicity and to increase opsonophagocytic uptake of Pseudomonas aeruginosa strain PAO1, serogroups 2 and 6. The results showed that PEIF can induce antibodies not only to neutralize the PE cytotoxicity but also to promote the uptake of various strains of P. aeruginosa by murine peritoneal macrophages. In a burned mouse model, PEIF afforded significant protection against infection by the homologous P. aeruginosa strain PAO1, heterologous serogroup 2, and the PE hyperproducing strain PA103. These observations thus indicate that PEIF may be used as a novel vaccine against P. aeruginosa infection.

Original languageEnglish
Pages (from-to)524-533
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume52
Issue number4
DOIs
Publication statusPublished - 1999

Fingerprint

Recombinant proteins
Vaccines
Recombinant Proteins
Membrane Proteins
Pseudomonas aeruginosa
Cytotoxicity
Proteins
Membranes
Antibodies
Pseudomonas Infections
Infection
Macrophages
Peritoneal Macrophages
Anti-Idiotypic Antibodies
OmpF protein
Pseudomonas aeruginosa toxA protein
Rabbits

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Recombinant protein composed of Pseudomonas exotoxin A, outer membrane proteins I and F as vaccine against P. aeruginosa infection. / Chen, T. Y.; Shang, H. F.; Chen, T. L.; Lin, C. P.; Hui, C. F.; Hwang, J.

In: Applied Microbiology and Biotechnology, Vol. 52, No. 4, 1999, p. 524-533.

Research output: Contribution to journalArticle

Chen, T. Y. ; Shang, H. F. ; Chen, T. L. ; Lin, C. P. ; Hui, C. F. ; Hwang, J. / Recombinant protein composed of Pseudomonas exotoxin A, outer membrane proteins I and F as vaccine against P. aeruginosa infection. In: Applied Microbiology and Biotechnology. 1999 ; Vol. 52, No. 4. pp. 524-533.
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