Recognition of the three deduced probable HLA haplotypes that areassociated with HLA-C*16: 04:01 (A*33:03-B*44:02-C*16:04:01-DRB1*11:04:01 and A*24-B*44:02-C*16:04:01-DRB1*11:04) and HLA-B*15:109 (A*11-B*15:109-DRB1*04) in Taiwanese unrelated hematopoietic stem cell donors

Kuo Liang Yang, Reuy Ho Kao, Chin Lon Lin, Py Yu Lin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: HLA-C*16:04:01 and HLA-B*15:109 are two uncommon alleles at the HLA-C locus and HLA-B locus, respectively. The objective of this study is to report the deduced probable human leukocyte antigen (HLA) haplotypes associated with HLA-C*16:04:01 and HLA-B*15:109 among Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and methods: A sequence-based typing method was employed to confirm the two low incidence alleles observed. Polymerase chain reaction was performed to amplify exons 2 and 3 of the HLA-A, and HLA-B loci with group-specific primer sets. Amplicons were sequenced using a BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions according to the manufacturer's protocols. Results: The DNA sequence of C*16:04:01 is identical to that of C*16:01:01 in exons 2 and 3, except for two nucleotide substitutions at residues 538 (C->T) and 539 (A->G), which results in a single amino acid replacement at position 156 (glutamine->tryptophan). We deduced two probable HLA haplotypes that are found in association with C*16:04:01 as A*33:03-B*44:02-C*16:04:01-DRB1*11:04 and A*24-B*44:02-C*16:04:01-DRB1*11:04. The DNA sequence of B*15:109 is identical to B*15:27:01 in exons 2 and 3 except for one nucleotide substitution at residue 200 (C->T), which results in a single amino acid replacement at position 43 (proline->leucine). A probable HLA haplotype associated with B*15:109 was deduced to be A*11-B*15:109-DRB1*04. Conclusion: Information on the deduced HLA haplotypes that are found in association with the rare C*16:04:01 and B*15:109 alleles that we report here is useful for reference purposes at HLA testing laboratories and will help stem cell transplantation donor search coordinators when they are determining the likelihood of finding a compatible donor for patients bearing these two uncommon HLA alleles from unrelated bone marrow donor registries.

Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalTzu Chi Medical Journal
Volume26
Issue number3
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Keywords

  • Haplotypes
  • Hematopoietic stem cell
  • HLA
  • Sequence-based typing
  • Transplantation

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Recognition of the three deduced probable HLA haplotypes that areassociated with HLA-C*16: 04:01 (A*33:03-B*44:02-C*16:04:01-DRB1*11:04:01 and A*24-B*44:02-C*16:04:01-DRB1*11:04) and HLA-B*15:109 (A*11-B*15:109-DRB1*04) in Taiwanese unrelated hematopoietic stem cell donors'. Together they form a unique fingerprint.

  • Cite this