Recognition of the deduced probable HLA haplotypes associated with HLA low incidence alleles B*13: 50 (A*11:02-B*13:50-DRB1*07:01) and B*51:39 (A*02-B*51:39-DRB1*15; and A*11-B*51:39-DRB1*15) in Taiwanese unrelated hematopoietic stem cell donors

Kuo Liang Yang, Reuy Ho Kao, Chin Lon Lin, Py Yu Lin

Research output: Contribution to journalArticle

Abstract

Objectives: HLA-B*13:50 and -B*51:39 are two low incidence alleles in the HLA-B locus. The objective of this study is to report the deduced probable human leukocyte antigen (HLA) haplotypes in association with HLA-B*13:50 and -B*51:39 in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and Methods: A sequence-based typing method was used to confirm the two low incidence alleles observed. Polymerase chain reaction was performed to amplify exons 2 and 3 in the HLA-A and HLA-B loci and exon 2 in the HLA-DRB1 locus with group-specific primer sets. Amplicons were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions according to the manufacturer's protocols. Results: The DNA sequence of B*13:50 is identical to B*13:01:01 in exons 2 and 3, except for a one nucleotide substitution at residue 482 (A→T), which results in a one amino acid replacement at position 137 (aspartic acid→valine). We deduced the probable HLA haplotype in association with B*13:50 in Taiwanese as A*11:02-B*13:50-DRB1*07:01. The DNA sequence of B*51:39 is identical to B*51:01:03 in exons 2 and 3 except for two nucleotide exchanges at residue 226 (A→G) and residue 228 (A→G), which result in a one amino acid substitution at position 52 (isoleucine→valine).The probable HLA haplotypes associated with B*51:39 in Taiwanese may be deduced as A*02-B*51:39-DRB1*15 and A*11-B*51:39-DRB1*15. Conclusion: Information on the deduced HLA haplotypes in association with the low incidence B*13:50 and B*51:39 alleles that we report here is valuable for HLA testing laboratories for reference purposes and for stem cell transplantation donor search coordinators, to determine the likelihood of finding compatible donors in unrelated bone marrow donor registries for patients carrying these two uncommon HLA alleles.

Original languageEnglish
Pages (from-to)68-72
Number of pages5
JournalTzu Chi Medical Journal
Volume26
Issue number2
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Fingerprint

HLA Antigens
Hematopoietic Stem Cells
Haplotypes
Alleles
Incidence
Exons
Nucleotides
Bone Marrow
Tissue Donors
Unrelated Donors
Stem Cell Transplantation
Amino Acid Substitution
Registries
Amino Acids
Polymerase Chain Reaction

Keywords

  • Haplotypes
  • Hematopoietic stem cell
  • HLA
  • Sequence-based typing
  • Transplantation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{b21043cba06a4d02aee16594a0389aa0,
title = "Recognition of the deduced probable HLA haplotypes associated with HLA low incidence alleles B*13: 50 (A*11:02-B*13:50-DRB1*07:01) and B*51:39 (A*02-B*51:39-DRB1*15; and A*11-B*51:39-DRB1*15) in Taiwanese unrelated hematopoietic stem cell donors",
abstract = "Objectives: HLA-B*13:50 and -B*51:39 are two low incidence alleles in the HLA-B locus. The objective of this study is to report the deduced probable human leukocyte antigen (HLA) haplotypes in association with HLA-B*13:50 and -B*51:39 in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and Methods: A sequence-based typing method was used to confirm the two low incidence alleles observed. Polymerase chain reaction was performed to amplify exons 2 and 3 in the HLA-A and HLA-B loci and exon 2 in the HLA-DRB1 locus with group-specific primer sets. Amplicons were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions according to the manufacturer's protocols. Results: The DNA sequence of B*13:50 is identical to B*13:01:01 in exons 2 and 3, except for a one nucleotide substitution at residue 482 (A→T), which results in a one amino acid replacement at position 137 (aspartic acid→valine). We deduced the probable HLA haplotype in association with B*13:50 in Taiwanese as A*11:02-B*13:50-DRB1*07:01. The DNA sequence of B*51:39 is identical to B*51:01:03 in exons 2 and 3 except for two nucleotide exchanges at residue 226 (A→G) and residue 228 (A→G), which result in a one amino acid substitution at position 52 (isoleucine→valine).The probable HLA haplotypes associated with B*51:39 in Taiwanese may be deduced as A*02-B*51:39-DRB1*15 and A*11-B*51:39-DRB1*15. Conclusion: Information on the deduced HLA haplotypes in association with the low incidence B*13:50 and B*51:39 alleles that we report here is valuable for HLA testing laboratories for reference purposes and for stem cell transplantation donor search coordinators, to determine the likelihood of finding compatible donors in unrelated bone marrow donor registries for patients carrying these two uncommon HLA alleles.",
keywords = "Haplotypes, Hematopoietic stem cell, HLA, Sequence-based typing, Transplantation",
author = "Yang, {Kuo Liang} and Kao, {Reuy Ho} and Lin, {Chin Lon} and Lin, {Py Yu}",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/j.tcmj.2014.04.002",
language = "English",
volume = "26",
pages = "68--72",
journal = "Tzu Chi Medical Journal",
issn = "1016-3190",
publisher = "財團法人中華民國佛教慈濟慈善事業基金會",
number = "2",

}

TY - JOUR

T1 - Recognition of the deduced probable HLA haplotypes associated with HLA low incidence alleles B*13

T2 - 50 (A*11:02-B*13:50-DRB1*07:01) and B*51:39 (A*02-B*51:39-DRB1*15; and A*11-B*51:39-DRB1*15) in Taiwanese unrelated hematopoietic stem cell donors

AU - Yang, Kuo Liang

AU - Kao, Reuy Ho

AU - Lin, Chin Lon

AU - Lin, Py Yu

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Objectives: HLA-B*13:50 and -B*51:39 are two low incidence alleles in the HLA-B locus. The objective of this study is to report the deduced probable human leukocyte antigen (HLA) haplotypes in association with HLA-B*13:50 and -B*51:39 in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and Methods: A sequence-based typing method was used to confirm the two low incidence alleles observed. Polymerase chain reaction was performed to amplify exons 2 and 3 in the HLA-A and HLA-B loci and exon 2 in the HLA-DRB1 locus with group-specific primer sets. Amplicons were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions according to the manufacturer's protocols. Results: The DNA sequence of B*13:50 is identical to B*13:01:01 in exons 2 and 3, except for a one nucleotide substitution at residue 482 (A→T), which results in a one amino acid replacement at position 137 (aspartic acid→valine). We deduced the probable HLA haplotype in association with B*13:50 in Taiwanese as A*11:02-B*13:50-DRB1*07:01. The DNA sequence of B*51:39 is identical to B*51:01:03 in exons 2 and 3 except for two nucleotide exchanges at residue 226 (A→G) and residue 228 (A→G), which result in a one amino acid substitution at position 52 (isoleucine→valine).The probable HLA haplotypes associated with B*51:39 in Taiwanese may be deduced as A*02-B*51:39-DRB1*15 and A*11-B*51:39-DRB1*15. Conclusion: Information on the deduced HLA haplotypes in association with the low incidence B*13:50 and B*51:39 alleles that we report here is valuable for HLA testing laboratories for reference purposes and for stem cell transplantation donor search coordinators, to determine the likelihood of finding compatible donors in unrelated bone marrow donor registries for patients carrying these two uncommon HLA alleles.

AB - Objectives: HLA-B*13:50 and -B*51:39 are two low incidence alleles in the HLA-B locus. The objective of this study is to report the deduced probable human leukocyte antigen (HLA) haplotypes in association with HLA-B*13:50 and -B*51:39 in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and Methods: A sequence-based typing method was used to confirm the two low incidence alleles observed. Polymerase chain reaction was performed to amplify exons 2 and 3 in the HLA-A and HLA-B loci and exon 2 in the HLA-DRB1 locus with group-specific primer sets. Amplicons were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions according to the manufacturer's protocols. Results: The DNA sequence of B*13:50 is identical to B*13:01:01 in exons 2 and 3, except for a one nucleotide substitution at residue 482 (A→T), which results in a one amino acid replacement at position 137 (aspartic acid→valine). We deduced the probable HLA haplotype in association with B*13:50 in Taiwanese as A*11:02-B*13:50-DRB1*07:01. The DNA sequence of B*51:39 is identical to B*51:01:03 in exons 2 and 3 except for two nucleotide exchanges at residue 226 (A→G) and residue 228 (A→G), which result in a one amino acid substitution at position 52 (isoleucine→valine).The probable HLA haplotypes associated with B*51:39 in Taiwanese may be deduced as A*02-B*51:39-DRB1*15 and A*11-B*51:39-DRB1*15. Conclusion: Information on the deduced HLA haplotypes in association with the low incidence B*13:50 and B*51:39 alleles that we report here is valuable for HLA testing laboratories for reference purposes and for stem cell transplantation donor search coordinators, to determine the likelihood of finding compatible donors in unrelated bone marrow donor registries for patients carrying these two uncommon HLA alleles.

KW - Haplotypes

KW - Hematopoietic stem cell

KW - HLA

KW - Sequence-based typing

KW - Transplantation

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U2 - 10.1016/j.tcmj.2014.04.002

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