Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium

Ke Zen, Celia X.J. Chen, Yi Tien Chen, Rosemarie Wilton, Yuan Liu

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

Receptor for advanced glycation endproducts (RAGE) is an Ig superfamily cell surface receptor that interacts with a diverse array of ligands associated with inflammatory responses. In this study, we provide evidence demonstrating that RAGE is involved in inflammatory responses in the intestines. We showed that RAGE is expressed in intestinal epithelial cells, primarily concentrated at the lateral membranes close to the apical cell junction complexes. Although RAGE expression was low in epithelium under normal conditions, this protein was up-regulated after treatment with the inflammatory cytokines IFN-γ and/or TNF-α. RAGE expression was also elevated in colon tissue samples from patients with inflammatory bowel diseases. Using in vitro transmigration assays, we found that RAGE mediates neutrophil (polymorphonuclear leukocytes (PMN)) adhesion to, and subsequent migration across, intestinal epithelial monolayers. This activity appears to be mediated by the binding of RAGE to the PMN-specific β2 integrin CD11b/CD18. Thus, these results provide a novel mechanism for the regulation of PMN transepithelial migration and may suggest a new therapeutic target for intestinal inflammation.

Original languageEnglish
Pages (from-to)2483-2490
Number of pages8
JournalJournal of Immunology
Volume178
Issue number4
DOIs
Publication statusPublished - Feb 15 2007
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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